In the last decade, surface plasmon resonance (SPR) has become a very sensitive technique for real-time detection of chemical and biochemical targets in many application areas. Considering the important needs for analyzing biomolecular reactions through automated and miniaturized components, optical fiber sensors based on the SPR effects are presently considered as an alternative in the development of microsensors. In the present work, a microfluidic system associated with an optical fiber SPR sensor is developed and evaluated to monitor in real-time the sensitivity of optical fiber sensor to each kinetic reaction occurring at the surface. From the kinetic parameters obtained by our experimental measurements and then implemented in the numerical model, modeling allows us to demonstrate the potential of SPR optical fiber sensors for biological analysis.