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‘Sub-imbibed’ storage is not an option for extending longevity of recalcitrant seeds of the tropical species, Trichilia dregeana Sond.

  • Penelope J. Drew (a1), N.W. Pammenter (a1) and Patricia Berjak (a1)

Abstract

Recalcitrant seeds of Trichilia dregeana were stored at 16 or 25°C, either at the water content at which they were shed or partially dried. Although having been exposed to a short period (approx. 6 h) at temperatures up to 30°C prior to storage, seeds at the original water content maintained viability for several weeks at 16°C. However, storage of undried seeds at 25°C was deleterious within 8 d, indicating a chemical basis for degeneration of hydrated recalcitrant seeds. Seeds that had been mildly dehydrated to the relatively high axis level of 1.68 g H2O g—1 dry mass, while maintaining full germinability immediately after drying, exhibited only 4% viability after 8 d in storage at 16°C and had completely lost viability after the same storage period at 25°C. Ultrastructural features characterizing hydrated seeds included indications of enhanced activity associated with initial exposure to the elevated temperature as well as some signs of stress. However, over an effective 15 d storage period at 16°C, ultrastructural features showed the cells to have retained little damage and to have been in an enhanced state of activity commensurate with ongoing development towards germination. After a longer storage period, however, signs of damage, including indirect evidence for disarray of the cytoskeleton in some axis cells, became apparent in line with the declining seed viability. Immediately following dehydration from an average axis water content of 1.97 to 1.68 H2O g g—1(the sub-imbibed condition), some ultrastructural abnormalities were apparent, but the seeds remained 100% germinable. However, within the 8 d storage period in this sub-imbibed condition, a spectrum of severe ultrastructural degeneration, including indirect evidence of the collapse of the nucleoskeleton and extensive cell lysis, accompanied viability decline of the seeds to 4%.

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*Corrrespondence Fax: +27-31-260-1195 Email: berjak@biology.und.ac.za

References

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Berjak, P. (1996) The role of micro-organisms in deterioration during storage of recalcitrant and intermediate seeds. pp. 121126in Ouédraogo, A.-S.; Poulsen, K.; Stubsgaard, I. (Eds) Intermediate/recalcitrant tropical forest seeds. Rome, IPGRI.
Berjak, P., Farrant, J.M. and Pammenter, N.W. (1989) The basis of recalcitrant seed behaviour. pp. 89108in Taylorson, R.B. (Ed.) Recent advances in the development and germination of seeds. New York, Plenum Press.
Berjak, P., Pammenter, N.W. and Vertucci, C. (1992) Homoiohydrous (recalcitrant) seeds: Developmental status, desiccation sensitivity and the state of water in axes of Landolphia kirkii Dyer. Planta 186, 249261.
Berjak, P., Campbell, G.K., Farrant, J.M., Omondi-Oloo, W. and Pammenter, N.W. (1995) Responses of seeds of Azadirachta indica (neem) to short-term storage under ambient or chilled conditions. Seed Science and Technology 23, 779792.
Berjak, P., Mycock, D.J., Wesley-Smith, J., Dumet, D. and Watt, M.P. (1996) Strategies for in vitro conservation of hydrated germplasm. pp. 1952in Normah, M.N.; Narimah, M.K.; Clyde, M.M. (Eds) In vitro conservation of plant genetic resources. Kuala Lumpur, Malaysia, Percetakan Watan SDN.BHD.
Chin, H.F. and Roberts, E.H. (1980) Recalcitrant crop seeds. Kuala Lumpur, Malaysia, Tropical Press SDN.BDH.
Choinski, J.S. Jr. (1990) Aspects of viability and postgerminative growth in seeds of the tropical tree, Trichilia dregeana Sonder. Annals of Botany 66, 437442.
Côme, D. and Corbineau, F. (1996) Metabolic damage related to desiccation sensitivity. pp. 107120in Ouédraogo, A.-S.; Poulsen, K.; Stubsgaard, I. (Eds) Intermediate/recalcitrant tropical forest seeds. Rome, IPGRI.
Corbineau, F. and Côme, D. (1986a) Experiments on the storage of seeds and seedlings of Symphonia globulifera L.f. (Guttiferae). Seed Science and Technology 14, 585591.
Corbineau, F. and Côme, D. (1986b) Experiments on germination and storage of the seeds of two dipterocarp: Shorea roxburghii and Hopea odorata. The Malaysian Forester 49, 371381.
Corbineau, F. and Côme, D. (1988) Storage of recalcitrant seeds of four tropical species. Seed Science and Technology 16, 97103.
Engelmann, F. (1997) In vitro conservation methods. pp. 119161 in Callow, J.A.; Ford-Lloyd, B.V.; Newbury, H.J. (Eds) Biotechnology and plant genetic resources. New York, CAB International.
Farrant, J.M., Pammenter, N.W. and Berjak, P. (1986) The increasing desiccation sensitivity of recalcitrant Avicennia marina seeds with storage time. Physiologia Plantarum 67, 291298.
Farrant, J.M., Pammenter, N.W. and Berjak, P. (1989) Germination-associated events and the desiccation sensitivity of recalcitrant seeds–a study on three unrelated species. Planta 178, 189198.
Finch-Savage, W.E. and Clay, H.A. (1994) Water relations of germination in the recalcitrant seeds of Quercus robur L. Seed Science Research 4, 315322.
Hong, T.D. and Ellis, R.H. (1996) A protocol to determine seed storage behaviour. Rome, IPGRI.
King, M.W. and Roberts, E.H. (1980) Maintenance of recalcitrant seeds in storage. pp. 5389in Chin, H.F.; Roberts, E.H. (Eds) Recalcitrant crop seeds. Kuala Lumpur, Malaysia, Tropical Press SDN.BHD.
Kioko, J., Berjak, P., Pammenter, N.W., Watt, M.P. and Wesley-Smith, J. (1998) Desiccation and cryopreservation of embryonic axes of Trichilia dregeana Sond. CryoLetters 19, 514.
Moreno Díaz de la Espina, S. (1995) Nuclear matrix isolated from plant cells. International Review of Cytology 162B, 75139.
Pammenter, N.W. and Berjak, P. (1999) A review of recalcitrant seed physiology in relation to desiccationtolerance mechanisms. Seed Science Research 9, 1337.
Pammenter, N.W., Berjak, P., Farrant, J.M., Smith, M.T. and Ross, G. (1994) Why do stored, hydrated recalcitrant seeds die? Seed Science Research 4, 187191.
Pammenter, N.W., Greggains, V., Kioko, J.I., Wesley-Smith, J., Berjak, P. and Finch-Savage, W.E. (1998) Effects of differential drying rates on viability retention of recalcitrant seeds of Ekebergia capensis. Seed Science Research 8, 463471.
Pooley, E. (1993) The complete field guide to trees of Natal, Zululand and Transkei. Durban, South Africa, Natal Flora Publications Trust.
Staehelin, L.A. (1997) The plant ER: a dynamic organelle composed of a large number of discrete functional domains. The Plant Journal 11, 11511165.
Staiger, C.J., Gibbon, B.C., Kovar, D.R. and Zonia, L.E. (1997) Profilin and actin-polymerizing factor: modulators of actin organization in plants. Trends in Plant Science 2, 275281.
Tompsett, P.B. and Pritchard, H.W. (1998) The effect of chilling and moisture status on the germination, desiccation and longevity of Aesculus hippocastanum L. seed. Annals of Botany 82, 249261.

Keywords

‘Sub-imbibed’ storage is not an option for extending longevity of recalcitrant seeds of the tropical species, Trichilia dregeana Sond.

  • Penelope J. Drew (a1), N.W. Pammenter (a1) and Patricia Berjak (a1)

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