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Reduced embryo sensitivity to abscisic acid in a sprouting-susceptible sorghum (Sorghum bicolor) variety is associated with altered ABA signalling

  • Nicolás Gualano (a1), Fernando Carrari (a2), María Verónica Rodríguez (a1), Laura Pérez-Flores (a3), Rodolfo Sánchez (a4), Norberto Iusem (a5) and Roberto Benech-Arnold (a1)...


In the work reported in this paper, we attempted to elucidate the nature of the different abscisic acid (ABA) sensitivities presented by developing embryos from sorghum [Sorghum bicolor (L.) Moench] lines with contrasting pre-harvest sprouting (PHS) behaviour (Redland B2, susceptible; IS 9530, resistant). We explored two different hypotheses for a possible mechanism: (1) a different functionality of the ABA signalling pathway, and (2) a different rate of ABA degradation/conjugation in the apoplast of embryos from these genotypes. To assess the first possibility, we used an ABA-responsive gene (Rab17) as a reporter of changes in endogenous ABA content, which were artificially induced in embryos from both genotypes by means of fluridone application immediately after anthesis, to reduce ABA content, and embryo incubation in the presence of ABA. A defect in ABA signalling should be seen as a level of Rab17 expression that is independent of endogenous ABA content. For testing the second possibility at two stages of development, embryos from both lines were isolated and incubated in water for different periods. ABA concentrations in embryos and the incubation media were quantified through radioimmunoassay. In contrast to our findings for the resistant IS 9530 line, Rab17 expression did not respond to changes in ABA levels in sensitive Redland B2 embryos. The ABA degradation/conjugation rates in embryos and incubation media did not show clear differences between sorghum lines for any of the developmental stages analysed. These results suggest that a disruption in the ABA signal transduction pathway in Redland B2 underlies the low ABA sensitivity shown by embryos from this line.


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Carrari, F., Pérez-Flores, L., Lijavetzky, D., Enciso, S., Sánchez, R., Benech-Arnold, R. and Iusem, N. (2001) Cloning and expression of a sorghum gene with homology to maize vp1. Its potential involvement in pre-harvest sprouting resistance. Plant Molecular Biology 45, 631640.
Carrari, F., Benech-Arnold, R., Osuna-Fernández, R., Hopp, E., Sánchez, R., Iusem, N. and Lijavetzky, D. (2003) Genetic mapping of the Sorghum bicolor vp1 gene and its relationship with preharvest sprouting resistance. Genome 46, 253258.
Finkelstein, R.R., Gampala, S.S.L. and Rock, C.D. (2002) Abscisic acid signaling in seeds and seedlings. Plant Cell 14, S15S45.
Fong, F., Smith, J.D. and Koehler, D.E. (1983) Early events in maize seed development. 1-methyl-3-phenyl-5-(3-[trifluoromethyl]phenyl)-4-(1h)-pyridinone induction of vivipary. Plant Physiology 73, 899901.
Giraudat, J., Hauge, B.M., Valon, C., Smalle, J., Parcy, F. and Goodman, H.M. (1992) Isolation of the Arabidopsis ABI3 gene by positional cloning. Plant Cell 4, 12511261.
Gubler, F., Millar, A.A. and Jacobsen, J.V. (2005) Dormancy release, ABA and pre-harvest sprouting. Current Opinion in Plant Biology 8, 183187.
Karssen, C.M., Brinkhorst-Van der Swan, D.L.C., Breekland, A.E. and Koornneef, M. (1983) Induction of dormancy during seed development by endogenous abscisic acid: studies on abscisic acid deficient genotypes of Arabidopsis thaliana (L.) Heynh. Planta 157, 158165.
Krochko, J.E., Abrams, G.D., Loewen, M.K., Abrams, S.R. and Cutler, A.J. (1998) (+)-Abscisic acid 8′-hydroxylase is a cytochrome P450 monooxygenase. Plant Physiology 118, 849860.
Kushiro, T., Okamoto, M., Nakabayashi, K., Yamagishi, K., Kitamura, S., Asami, T., Hirai, N., Koshiba, T., Kamiya, Y. and Nambara, E. (2004) The Arabidopsis cytochrome P450 CYP707A encodes ABA 8′-hydroxylases: key enzymes in ABA catabolism. EMBO Journal 23, 16471656.
McKibbin, R.S., Wilkinson, M.D., Bailey, P.C., Flintham, J.E., Andrew, L.M., Lazzeri, P.A., Gale, M.D., Lenton, J.R. and Holdsworth, M.J. (2002) Transcripts of Vp-1 homeologues are misspliced in modern wheat and ancestral species. Proceedings of the National Academy of Sciences, USA 15, 1020310208.
Quarrie, S.A., Whitford, P.N., Appleford, N.E.J., Wang, T.L., Cook, S.K., Henson, I.E. and Loveys, B.R. (1988) A monoclonal antibody to (S)-abscisic acid: its characterization and use in a radioimmunoassay for measuring abscisic acid in crude extracts of cereal and lupine leaves. Planta 173, 330339.
Razem, F.A., El-Kereamy, A., Abrams, S.R. and Hill, R.D. (2006) The RNA-binding protein FCA is an abscisic acid receptor. Nature 439, 290294.
Riera, M., Figueras, M., Lopez, C., Goday, A. and Pages, M. (2004) Protein kinase CK2 modulates developmental functions of the abscisic acid responsive protein RAB17 from maize. Proceedings of the National Academy of Sciences, USA 101, 98799884.
Robichaud, C.S., Wong, J. and Sussex, I.M. (1980) Control of in vitro growth of viviparous embryo mutants of maize by abscisic acid. Developmental Genetics 1, 325330.
Sambrook, J. and Russell, D.W. (2001) Molecular cloning: A laboratory manual (3rd edition). Cold Spring Harbor, New York, Cold Spring Harbor Laboratory Press.
Shen, Y.Y., Wang, X.F., Wu, F.Q., Du, S.Y., Cao, Z., Shang, Y., Wang, X.L., Peng, C.C., Yu, X.C., Zhu, S.Y., Fan, R.C., Xu, Y.H. and Zhang, D.P. (2006) The Mg-chelatase H subunit is an abscisic acid receptor. Nature 443, 823826.
Steinbach, H.S., Benech-Arnold, R.L., Kristof, G., Sánchez, R.A. and Marcucci Poltri, S. (1995) Physiological basis of pre-harvest sprouting resistance in Sorghum bicolor (L.) Moench. ABA levels and sensitivity in developing embryos of sprouting resistant and susceptible varieties. Journal of Experimental Botany 46, 701709.
Steinbach, H.S., Benech-Arnold, R.L. and Sánchez, R.A. (1997) Hormonal regulation of dormancy in developing Sorghum seeds. Plant Physiology 113, 149154.
Trewavas, A. (2000) Signal perception and transduction. pp. 930986in Buchanan, B.B.; Gruissem, W.; Jones, R.L. (Eds) Biochemistry and molecular biology of plants. Rockville, Maryland, American Society of Plant Physiologists.
Visser, K., Vissers, A.P.A., Cagirgan, M.I., Kijne, J.W. and Wang, M. (1996) Rapid germination of a barley mutant is correlated with a rapid turnover of abscisic acid outside the embryo. Plant Physiology 111, 11271133.
Xu, N., Coulter, K.M. and Bewley, J.D. (1990) Abscisic acid and osmoticum prevent germination of developing alfalfa embryos, but only osmoticum maintains the synthesis of developmental proteins. Planta 182, 382390.


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Reduced embryo sensitivity to abscisic acid in a sprouting-susceptible sorghum (Sorghum bicolor) variety is associated with altered ABA signalling

  • Nicolás Gualano (a1), Fernando Carrari (a2), María Verónica Rodríguez (a1), Laura Pérez-Flores (a3), Rodolfo Sánchez (a4), Norberto Iusem (a5) and Roberto Benech-Arnold (a1)...


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