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Is oxidative stress involved in the loss of neem (Azadirachta indica) seed viability?

  • Moctar Sacandé (a1) (a2) (a3), Folkert A. Hoekstra (a2), Adriaan C. van Aelst (a4) and C.H. Ric De Vos (a3)

Abstract

Neem (Azadirachta indica) is a valuable multipurpose tree of tropical arid and semi-arid regions. The use of its seeds is hindered by their short storage longevity. The possible causes of rapid loss of viability were investigated on different seed lots during exposure to 32% and 75% RH at 20°C. Within 6 months the seeds almost lost germinability at 75% RH, whereas at 32% RH viability decreased only slightly. On rehydration, the axis cells from nongerminable seeds had lost turgor, whereas those from viable seeds were turgescent as visualized by low temperature scanning electron microscopy images of fractured axes. Glutathione oxidation status was used to estimate oxidative stress during storage. Oxidative stress was much higher at 75% RH storage than at 32% RH, mainly caused by the rapid loss of reduced glutathione at 75% RH. Oligosaccharides and phospholipids decreased, and free fatty acids increased during storage at the high RH but remained at a constant level at the low RH. However, the degree of fatty acid unsaturation between viable and nonviable seed lots was similar. During the (slow) dehydration of fresh seeds, total glutathione, oligosaccharides and phospholipids accumulated, particularly in the initially more hydrated seeds. We interpret this accumulation as a post-maturation process associated with acquisition of the capability for long-term survival in the dry state. The mass ratio of oligosaccharides to sucrose was 0.19 on average in dehydrated neem seeds. The data suggest that the storage behaviour of neem seeds has features that characterize it as orthodox.

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Corresponding author

*Correspondence: Laboratory of Plant Physiology, Wageningen University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands Fax: +31-317-484740 Email: moctar.sacande@algem.pf.wau.nl

References

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Anderson, M.E. (1985) Tissue glutathione. pp. 317323 in Greenwald, R.A. (Ed.) Handbook of methods for oxygen free radical research. Boca Raton, CRC Press.
Benson, E.E. (1990) Free radical damage in stored plant germplasm. pp. 3768. Rome, International Board of Plant Genetic Resources (IBPGR).
Bowler, C., Van Montagu, M. and Inze, D. (1992) Superoxide dismutase and stress tolerance. Annual Review of Plant Physiology and Plant Molecular Biology 43, 83116.
Buitink, J., Walters, C., Hoekstra, F.A. and Crane, J. (1998) Storage behaviour of Typha latifolia pollen at low water contents: Interpretation on the basis of water activity and glass concepts. Physiologia Plantarum 103, 145153.
Carpenter, J.F. and Crowe, J.H. (1989) An infrared spectroscopic study of the interactions of carbohydrates with dried proteins. Biochemistry 28, 39163922.
Crowe, J.H., Hoekstra, F.A. and Crowe, L.M. (1992) Anhydrobiosis. Annual Review of Physiology 54, 579599.
De Vos, C.H.R., Vonk, M.J., Vooijs, R. and Schat, H. (1992) Glutathione depletion due to copper-induced phytochelatin synthesis causes oxidative stress in Silene cucubalus. Plant Physiology 98, 853858.
De Vos, C.H.R., Kraak, H.L. and Bino, R.J. (1994) Ageing of tomato seeds involves glutathione oxidation. Physiologia Plantarum 92, 131139.
Ellis, R.H., Hong, T.D. and Roberts, E.H. (1990) An intermediate category of seed storage behaviour? I. Coffee. Journal of Experimental Botany 41, 11671174.
Ellis, R.H., Hong, T.D. and Roberts, E.H. (1991) Effect of storage temperature and moisture on the germination of papaya seeds. Seed Science Research 1, 6972.
Ellman, G.L. (1959) Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics 82, 7077.
Finch-Savage, W.E., Hendry, G.A.F. and Atherton, N.M. (1994) Free radical activity and loss of viability during drying of desiccation-sensitive tree seeds. Proceedings of the Royal Society of Edinburgh Section B 102, 257260.
Gaméné, C.S., Kraak, H.L., Van Pijlen, J.G. and De Vos, C.H.R. (1996) Storage behaviour of neem (Azadirachta indica) seeds from Burkina Faso. Seed Science and Technology 24, 441448.
Hendry, G.A.F., Finch-Savage, W.E., Thorpe, P.C., Atherton, N.M., Buckland, S.M., Nilsson, K.A. and Seel, W.E. (1992) Free radical processes and loss of seed viability during desiccation in the recalcitrant species Quercus robur L. New Phytologist 122, 273279.
Hoekstra, F.A., Crowe, L.M. and Crowe, J.H. (1989) Differential desiccation sensitivity of corn and Pennisetum pollen linked to their sucrose contents. Plant, Cell and Environment 12, 8391.
Hong, T.D. and Ellis, R.H. (1998) Contrasting seed storage behaviour among different species of Meliaceae. Seed Science and Technology 26, 7795.
Hong, T.D., Linington, S. and Ellis, R.H. (1996) Seeds storage behaviour: a compendium. Handbooks for genebanks No 4. Rome, Italy, International Plant Genetic Resources Institute (IPGRI).
Horbowicz, M. and Obendorf, R.L. (1994) Seed desiccation tolerance and storability: dependence on flatulence-producing oligosaccharides and cyclitols-review and survey. Seed Science Research 4, 385405.
ISTA (International Seed Testing Association) (1993) International rules for seed testing 1993. Seed Science and Technology 21, 2530, 37–41.
Kranner, I. and Grill, D. (1996) Significance of thiol-disulfide exchange in resting stages of plant development. Botanica Acta 109, 814.
Leprince, O., Atherton, N.M., Deltour, R. and Hendry, G.A.F. (1994) The involvement of respiration in free radical processes during loss of desiccation tolerance in germinating Zea mays L. – an electron paramagnetic study. Plant Physiology 104, 13331339.
Leprince, O., Deltour, R., Thorpe, P.C., Atherton, N.M. and Hendry, G.A.F. (1990) The role of free radicals and radical processing systems in loss of desiccation tolerance in germinating maize (Zea mays L.). New Phytologist 116, 573580.
Magill, W., Deighton, N., Pritchard, H.W., Benson, E.E. and Goodman, B.A. (1993) Physiological and biochemical studies of seed storage parameters in Carica papaya. Proceedings of the Royal Society of Edinburgh, Section B 102, 439442.
Maithani, G.P., Bahuguna, V.K., Rawat, M.M.S. and Sood, O.P. (1989) Fruit maturity and interrelated effects of temperature and container on longevity of neem (Azadirachta indica) seeds. Indian Forester 115, 8997.
Navari-Izzo, F., Meneguzzo, S., Loggini, B., Vazzana, C. and Sgherri, C.L.M. (1997) The role of the glutathione system during dehydration of Boea hygroscopica. Physiologia Plantarum 99, 2330.
Pammenter, N.W. and Berjak, P. (1999) A review of recalcitrant seed physiology in relation to desiccation-tolerance mechanisms. Seed Science Research 9, 1337.
Ponnuswamy, A.S., Vinaya Rai, R.S., Surendran, C. and Karivaratharaju, T.V. (1990) Studies on maintaining seed longevity and the effect of fruit grades in neem (Azadirachta indica). Journal of Tropical Forest Science 3, 285290.
Poulsen, K. (1996) Case study of neem (Azadirachta indica) seeds. pp. 101104 in Ouédraogo, A.S.; Poulsen, K.; Stubsgaard, F. (Eds) Improved methods for the handling and storage of intermediate/recalcitrant tropical forest tree seeds. Rome, International Plant Genetic Resources Institute (IPGRI).
Priestley, D.A. and Leopold, A.C. (1979) Absence of lipid oxidation during accelerated aging of soybean seeds. Plant Physiology 63, 726729.
Roberts, E.H. (1973) Predicting the storage life of seeds. Seed Science and Technology 1, 499514.
Roberts, E.H. and Ellis, R.H. (1989) Water and seed survival. Annals of Botany 63, 3952.
Sanhewe, A.J. and Ellis, R.H. (1996a) Seed development and maturation in Phaseolus vulgaris. I. Ability to germinate and to tolerate desiccation. Journal of Experimental Botany 47, 949958.
Sanhewe, A.J. and Ellis, R.H. (1996b) Seed development and maturation in Phaseolus vulgaris. II. Post-harvest longevity in air-dry storage. Journal of Experimental Botany 47, 959965.
Sacandé, M., Buitink, J. and Hoekstra, F.A. (2000) A study of water relations in neem (Azadirachta indica) seed that is characterised by complex storage behaviour. Journal of Experimental Botany 51, 635643.
Sacandé, M., Hoekstra, F.A., Van Pijlen, J.G. and Groot, S.P.C. (1998) A multifactorial study on conditions influencing storage longevity of neem (Azadirachta indica) seeds. Seed Science Research 8, 473482.
Sacandé, M., Van Pijlen, J.G., De Vos, C.H.R., Hoekstra, F.A., Bino, R.J. and Groot, S.P.C. (1996) Intermediate storage behaviour of neem tree (Azadirachta indica) seeds from Burkina Faso. pp. 101104in Ouédraogo, A.S.; Poulsen, K.; Stubsgaard, F. (Eds) Improved methods for the handling and storage of intermediate/recalcitrant tropical forest tree seeds. Rome, International Plant Genetic Resources Institute (IPGRI).
Senaratna, T. and McKersie, B.D. (1986) Loss of desiccation tolerance during seed germination: a free radical mechanism of injury. pp. 85101in Leopold, A.C. (Ed.) Membranes, metabolism and dry organisms. Ithaca, NY, Cornell University Press.
Senaratna, T., Gusse, J.F. and McKersie, B.D. (1988) Age-induced changes in cellular membranes of imbibed soybean seed axes. Physiologia Plantarum 73, 8591.
Sinniah, U.R., Ellis, R.H. and John, P. (1998) Irrigation and seed quality development in rapid-cycling brassica: soluble carbohydrates and heat-stable proteins. Annals of Botany 82, 647655.
Steadman, K.J., Pritchard, H.W. and Dey, P.M. (1996) Tissue-specific soluble sugars in seeds as indicators of storage category. Annals of Botany 77, 667674.
Sun, W.Q. (1997) Glassy state and seed storage stability: The WLF kinetics of seed viability loss at TT g and the plasticization effect of water on storage stability. Annals of Botany 79, 291297.
Tompsett, P.B. and Kemp, R. (1996) Database of tropical tree seed research, with special reference to the Dipterocarpaceae, Meliaceae and Araucariaceae: database contents. pp. 1263. Wakehurst Place, Ardingly, Royal Botanic Gardens.
Van Bilsen, D.G.J.L. and Hoekstra, F.A. (1993) Decreased membrane integrity in aging Typha latifolia L. pollen: accumulation of lysolipids and free fatty acids. Plant Physiology 101, 675682.
Van Bilsen, D.G.J.L., Van Roekel, T. and Hoekstra, F.A. (1994) Declining viability and lipid degradation during pollen storage. Sexual Plant Reproduction 7, 303310.
Wilson, D.O. and McDonald, M.B. (1986) The lipid peroxidation model of seed ageing. Seed Science and Technology 14, 269300.
Wolkers, W.F., Tetteroo, F.A.A., Alberda, M. and Hoekstra, F.A. (1999) Changed properties of the cytoplasmic matrix associated with desiccation tolerance of dried carrot somatic embryos. An in situ Fourier transform infrared study. Plant Physiology 120, 153163.
Wolkers, W.F., Van Kilsdonk, M.G. and Hoekstra, F.A. (1998) Dehydration-induced conformational changes of poly-L-lysine as influenced by drying rate and carbohydrates. Biochimica et Biophysica Acta 1425, 127136.

Keywords

Is oxidative stress involved in the loss of neem (Azadirachta indica) seed viability?

  • Moctar Sacandé (a1) (a2) (a3), Folkert A. Hoekstra (a2), Adriaan C. van Aelst (a4) and C.H. Ric De Vos (a3)

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