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Desiccation sensitivity and cell cycle aspects in seeds of Inga vera subsp. affinis

  • José Marcio Rocha Faria (a1) (a2), André A.M. van Lammeren (a3) and Henk W.M. Hilhorst (a1)

Abstract

The desiccation sensitivity of seeds of Inga vera Willd. subsp. affinis, a recalcitrant-seeded tree from Brazil, was analysed, focusing on water relations and cell-cycle aspects, including DNA content and the microtubular cytoskeleton. Seeds were collected at four developmental stages, dried to different moisture contents (MCs), assessed regarding water activity and set to germinate. Samples of fresh (non-dried) developing and mature seeds were used for assessment of DNA content by flow cytometry. Immunohistochemical detection of microtubules (MTs) was done in mature seeds at different MCs. Slight desiccation of immature seeds increased germination, but further drying resulted in a quick decline of germinability. During seed development the desiccation sensitivity decreased slightly, but DNA content of the embryonic axis cells remained constant, suggesting no relation between those two parameters. Embryonic axis cells of mature seeds showed abundant cortical microtubule arrays, which were not affected by mild desiccation, but broken down by further drying. It appeared that, upon rehydration, damaged cells were not able to reconstitute the microtubular cytoskeleton. The failure of germination of Inga vera seeds after drying could not be attributed to cellular damage to DNA synthesis and mitosis, since the radicle protruded by means of cell elongation, without a need for cell division. However, the breakdown of MTs during desiccation, and their subsequent inability to reassemble upon rehydration, may be related to the decreased germination, since MTs are required for cell elongation.

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*Correspondence Fax: +31 317 484740, Email: henk.hilhorst@wur.nl

References

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Alberts, B., Bray, D., Johnson, A., Lewis, J., Raff, M., Roberts, K. and Walter, P. (1998) Essential cell biology: An introduction to the molecular biology of the cell. New York, Garland Publishing.
Amaral, da and Silva, E.A. (2002) Coffee seed (Coffea arabica cv. Rubi) germination: mechanism and regulation. PhD Thesis, Wageningen University, The Netherlands.
Arumuganathan, K. and Earle, E.D. (1991) Estimation of nuclear DNA content of plants by flow cytometry. Plant Molecular Biology Reporter 9, 229241.
Baskin, T.I., Busby, C.H., Fowke, L.C., Sammut, M. and Gubler, F. (1992) Improvements in immunostaining samples embedded in methacrylate: localization of microtubules and other antigens throughout developing organs in plants of diverse taxa. Planta 187, 405413.
Berjak, P. and Pammenter, N.W. (1994) Recalcitrance is not an all-or-nothing situation. Seed Science Research 4, 263264.
Berjak, P. and Pammenter, N.W. (2000) What ultrastructure has told us about recalcitrant seeds. Revista Brasileira de Fisiologia Vegetal 12, 2255.
Berjak, P., Pammenter, N.W. and Vertucci, C. (1992) Homoiohydrous (recalcitrant) seeds: developmental status, desiccation sensitivity and the state of water in axes of Landolphia kirkii Dyer. Planta 186, 249261.
Berjak, P., Vertucci, C.W. and Pammenter, N.W. (1993) Effects of developmental status and dehydration rate on characteristics of water and desiccation-sensitivity in recalcitrant seeds of Camellia sinensis. Seed Science Research 3, 155166.
Berjak, P., Kioko, J.I., Walker, M., Mycock, D.J., Wesley-Smith, J., Watt, P. and Pammenter, N.W. (1999) Cryopreservation: an elusive goal. pp. 96109in Marzalina, M., Khoo, K.C., Jayanthi, N., Tsan, F.Y. and Krishnapillay, B. (Eds) IUFRO seed symposium 1998. Recalcitrant seeds. Proceedings of the conference. Kuala Lumpur, Forest Research Institute Malaysia.
Bewley, J.D. and Black, M. (1994) Seeds. Physiology of development and germination (2nd edition). New York, Plenum Press.
Bilia, D.A.C., Marcos, Filho, J. and Novembre, A.D.C.L. (1999) Desiccation tolerance and seed storability of Inga uruguensis (Hook. et Arn.). Seed Science and Technology 27, 7789.
Bino, R.J., Lanteri, S., Verhoeven, H.A. and Kraak, H.L. (1993) Flow cytometric determination of nuclear replication stages in seed tissues. Annals of Botany 72, 181187.
Boubriak, I., Dini, M., Berjak, P. and Osborne, D.J. (2000) Desiccation and survival in the recalcitrant seeds of Avicennia marina: DNA replication, DNA repair and protein synthesis. Seed Science Research 10, 307315.
Bouvier-Durand, M., Real, M., Côme, D. (1989) Changes in nuclear activity upon secondary dormancy induction by abscisic acid in apple embryos. Plant Physiology and Biochemistry 27, 511518.
Chin, H.F. (1989) Recalcitrant seeds. Extension Bulletin No. 288. Taipei City, Taiwan ASPAC: Food and Fertilizer Technology Center.
Chin, H.F., Krishnapillay, B. and Stanwood, P.C. (1989) Seed moisture: recalcitrant vs. orthodox seeds. pp. 1522in Stanwood, P.C. and McDonald, M.B. (Eds) Seed moisture. CSSA Special Publication No. 14. Madison, Wisconsin Crop Science Society of America.
Corbineau, F. and Côme, D. (1988) Storage of recalcitrant seeds of four tropical species. Seed Science and Technology 16, 97103.
Davide, A.C., Faria, J.M.R. and Botelho, S.A. (1995) Propagação de espécies florestais. Technical Bulletin. Belo Horizonte, Brazil, CEMIG/UFLA/FAEPE.
de Castro, R.D., Bino, R.J., Jing, H.C., Kieft, H. and Hilhorst, H.W.M. (2001) Depth of dormancy in tomato (Lycopersicon esculentum Mill.) seeds is related to the progression of the cell cycle prior to the induction of dormancy. Seed Science Research 11, 4554.
Deltour, R. (1985) Nuclear activation during early germination of the higher plant embryo. Journal of Cell Science 75, 4383.
Dussert, S., Chabrillange, N., Engelmann, F. and Hamon, S. (1999) Quantitative estimation of seed desiccation sensitivity using a quantal response model: application to nine species of the genus Coffea L. Seed Science Research 9, 135144.
Dustin, P. (1978) Microtubules. Berlin, Springer-Verlag.
Ellis, R.H., Hong, T.D., Roberts., E.H. (1990) An intermediate category of seed storage behaviour? I. Coffee. Journal of Experimental Botany 41, 11671174.
Erdey, D.P., Pammenter, N.W., Finch-Savage, W.E. and Berjak, P. (2003) Physiological assessments of vigour enhancement by mild dehydration stress in recalcitrant seeds: effect of seed maturity. p. 16in Proceedings of the 4th international workshop on desiccation tolerance and sensitivity of seeds and vegetative plant tissues, August, Blouwaterbaai, South Africa.
Farnsworth, E. (2000) The ecology and physiology of viviparous and recalcitrant seeds. Annual Review of Ecology and Systematics 31, 107138.
Farrant, J.M., Berjak, P. and Pammenter, N.W. (1985) The effect of drying rate on viability retention of recalcitrant propagules of Avicennia marina. South African Journal of Botany 51, 432438.
Farrant, J.M., Pammenter, N.W. and Berjak, P. (1993) Seed development in relation to desiccation tolerance: a comparison between desiccation-sensitive (recalcitrant) seeds of Avicennia marina and desiccation-tolerant types. Seed Science Research 3, 113.
Finch-Savage, W.E. (1992) Embryo water status and survival in the recalcitrant species Quercus robur L.: evidence for a critical moisture content. Journal of Experimental Botany 43, 663669.
Finch-Savage, W.E., Bergervoet, J.H.W., Bino, R.J., Clay, H.A. and Groot, S.P.C. (1998) Nuclear replication activity during seed development, dormancy breakage and germination in three tree species: Norway maple (Acer platanoides L.), sycamore (Acer pseudoplatanus L.) and cherry (Prunus avium L.). Annals of Botany 81, 519526.
Goddard, R.H., Wick, S.M., Silflow, C.D. and Snustad, D.P. (1994) Microtubule components of the plant cell cytoskeleton. Plant Physiology 104, 16.
Grabe, D.F. (1989) Measurement of seed moisture. pp. 6992in Stanwood, P.C. and McDonald, M.B. (Eds) Seed moisture. CSSA Special Publication No. 14. Madison, USA Crop Science Society of America.
Gumede, Z., Merhar, V. and Berjak, P. (2003) Effect of desiccation on the microfilament component of the cytoskeleton in zygotic embryonic axes of Trichilia dregeanai Sond. p. 22in Proceedings of the 4th international workshop on desiccation tolerance and sensitivity of seeds and vegetative plant tissues, August, Blouwaterbaai, South Africa.
Hong, T.D. and Ellis, R.H. (1990) A comparision of maturation drying, germination, and desiccation tolerance between developing seeds of Acer pseudoplatanus L. and Acer platanoides L. New Phytologist 116, 589596.
Hong, T.D. and Ellis, R.H. (1992) Optimum air-dry storage environments for arabica coffee. Seed Science and Technology 20, 547560.
Hong, T.D. and Ellis, R.H. (1996) A protocol to determine seed storage behaviour. IPGRI Technical Bulletin No. 1. Rome, International Plant Genetic Resources Institute.
ISTA (International Seed Testing Association). (1996) International rules for seed testing. Seed Science and Technology 24. (suppl.).
King, M.W. and Roberts, E.H. (1979) The storage of recalcitrant seeds: achievements and possible approaches. Rome, International Board for Plant Genetic Resources.
Köppen, W. (1936) Das geographische system der klimate. pp. 144in Köppen, W. and Geiger, R. (Eds) Handbuch der Klimatologie Vol. 1, part C. Berlin, Gebrüder Borntraeger.
Kumagai, F. and Hasezawa, S. (2001) Dynamic organization of microtubules and microfilaments during cell cycle progression in higher plant cells. Plant Biology 3, 416.
Leopold, A.C. and Vertucci, C.W. (1986) Physical attributes of desiccated seeds. pp. 2234in Leopold, A.C. (Eds) Membranes, metabolism and dry organisms. Ithaca, Comstock Publishing.
Liang, Y. and Sun, W.Q. (2000) Desiccation tolerance of recalcitrant Theobroma cacao embryonic axes: the optimal drying rate and its physiological basis. Journal of Experimental Botany 51, 19111919.
Lloyd, C. (1994) Why should stationary plant cells have such dynamic microtubules? Molecular Biology of the Cell 5, 12771280.
Mycock, D.J., Berjak, P., Finch-Savage, W.E. (2000) Effects of desiccation on the subcellular matrix of the embryonic axes of Quercus robur. pp. 197203in Black, M., Bradford, K.J. and Vazquez-Ramos, J. (Eds) Seed biology: Advances and applications. Wallingford, CABI Publishing.
Oliver, M.J. (1996) Desiccation tolerance in vegetative plant cells. Physiologia Plantarum 97, 779787.
Pammenter, N.W. and Berjak, P. (1999) A review of recalcitrant seed physiology in relation to desiccation-tolerance mechanisms. Seed Science Research 9, 1337.
Pammenter, N.W. and Berjak, P. (2000) Aspects of recalcitrant seed physiology. Revista Brasileira de Fisiologia Vegetal 12, 5669.
Pammenter, N.W., Vertucci, C.W. and Berjak, P. (1991) Homeohydrous (recalcitrant) seeds: dehydration, the state of water and viability characteristics in Landolphia kirkii. Plant Physiology 96, 10931098.
Pammenter, N.W., Berjak, P., Farrant, J.M., Smith, M.T. and Ross, G. (1994) Why do stored hydrated recalcitrant seeds die? Seed Science Research 4, 187191.
Pammenter, N.W., Greggains, V., Kioko, J.I., Wesley-Smith, J., Berjak, P., Finch-Savage, W.E. (1998) Effects of differential drying rates on viability retention of recalcitrant seeds of Ekebergia capensis. Seed Science Research 8, 463471.
Pammenter, N.W., Berjak, P. and Walters, C. (2000) The effect of drying rate on recalcitrant seeds: ‘lethal water contents’, causes of damage, and quantification of recalcitrance. pp. 215221in Black, M., Bradford, K.J. and Vazquez-Ramos, J. (Eds) Seed biology: Advances and applications. Wallingford, CABI Publishing.
Pennington, T.D. (1997) The genus Inga: Botany. Richmond, The Royal Botanic Gardens.
Poulsen, K.M. and Eriksen, E.N. (1992) Physiological aspects of recalcitrance in embryonic axes of Quercus robur L. Seed Science Research 2, 215221.
Pritchard, H.W. (1991) Water potential and embryonic axis viability in recalcitrant seeds of Quercus rubra. Annals of Botany 67, 4349.
Pritchard, H.W., Haye, A.J., Wright, W.J. and Steadman, K.J. (1995) A comparative study of seed viability in Inga species: desiccation tolerance in relation to the physical characteristics and chemical composition of the embryo. Seed Science and Technology 23, 85100.
Probert, R.J. and Longley, P.L. (1989) Recalcitrant seed storage physiology in three aquatic grasses (Zizania palustris, Spartina anglica and Porteresia coarctata). Annals of Botany 63, 5363.
Roberts, E.H. (1973) Predicting the storage life of seeds. Seed Science and Technology 1, 499514.
Roberts, E.H. and Ellis, R.H. (1989) Water and seed survival. Annals of Botany 63, 3952.
Sacandé, M. (2000) Stress, storage and survival of neem seed. PhD Thesis, Wageningen Agricultural University, The Netherlands.
Saracco, F., Bino, R.J., Bergervoet, J.H.W. and Lanteri, S. (1995) Influence of priming-induced nuclear replication activity on storability of pepper (Capsicum annuum L.) seed. Seed Science Research 5, 2529.
Sargent, J.A., Sen Mandi, S. and Osborne, D.J. (1981) The loss of desiccation tolerance during germination: an ultrastructural and biochemical approach. Protoplasma 105, 225239.
Sun, W.Q. (2002) Methods for the study of water relations under desiccation stress. pp. 4791in Black, M. and Pritchard, H.W. (Eds) Desiccation and survival in plants: Drying without dying. Wallingford, CABI Publishing.
Sun, W.Q. and Gouk, S.S. (1999) Preferred parameters and methods for studying moisture content of recalcitrant seeds. pp. 404430in Marzalina, M., Khoo, K.C., Jayanthi, N., Tsan, F.Y. and Krishnapillay, B. (Eds) IUFRO seed symposium 1998. Recalcitrant seeds. Proceedings of the conference. Kuala Lumpur, Forest Research Institute Malaysia.
Tompsett, P.B. (1984) Desiccation studies in relation to the storage of Araucaria seed. Annals of Applied Biology 105, 581586.
Uniyal, R.C. and Nautiyal, A.R. (1996) Physiology of seed development in Aesculus indica, a recalcitrant seed. Seed Science and Technology 24, 419424.
Vantard, M., Cowling, R. and Delichère, C. (2000) Cell cycle regulation of the microtubular cytoskeleton. Plant Molecular Biology 43, 691703.
Vazquez-Ramos, J.M. and Sanchez, M.D. (2003) The cell cycle and seed germination. Seed Science Research 13, 113130.
Vertucci, C.W. and Leopold, A.C. (1987a) The relationship between water binding and desiccation tolerance in tissues. Plant Physiology 85, 232238.
Vertucci, C.W. and Leopold, A.C. (1987b) Water binding in legume seeds. Plant Physiology 85, 224231.
Walters, C., Farrant, J.M., Pammenter, N.W. and Berjak, P. (2002) Desiccation stress and damage. pp. 263291in Black, M. and Pritchard, H.W. (Eds) Desiccation and survival in plants: Drying without dying. Wallingford, CABI Publishing.
Wesley-Smith, J., Pammenter, N.W., Berjak, P. and Walters, C. (2001) The effects of two drying rates on the desiccation tolerance of embryonic axes of recalcitrant jackfruit (Artocarpus heterophyllus Lamk.) seeds. Annals of Botany 88, 653664.
Yuan, M., Shaw, P.J., Warn, R.M. and Lloyd, C.W. (1994) Dynamic reorientation of cortical microtubules, from transverse to longitudinal, in living plant cells. Proceedings of the National Academy of Sciences, USA 91, 60506053

Keywords

Desiccation sensitivity and cell cycle aspects in seeds of Inga vera subsp. affinis

  • José Marcio Rocha Faria (a1) (a2), André A.M. van Lammeren (a3) and Henk W.M. Hilhorst (a1)

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