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Desiccation sensitivity and cell cycle aspects in seeds of Inga vera subsp. affinis

Published online by Cambridge University Press:  22 February 2007

José Marcio Rocha Faria ,
André A.M. van Lammeren  and
Henk W.M. Hilhorst
José Marcio Rocha Faria
Affiliation:
Laboratory of Plant Physiology, Wageningen University, Arboretumlaan 4, Wageningen, 6703 BD, The Netherlands Departamento de Ciências Florestais, Universidade Federal de Lavras, CP 37, Lavras, MG, CEP 37200-000, Brazil
André A.M. van Lammeren
Affiliation:
Laboratory of Plant Cell Biology, Wageningen University, Arboretumlaan 4, Wageningen, 6703 BD, The Netherlands
Henk W.M. Hilhorst*
Affiliation:
Laboratory of Plant Physiology, Wageningen University, Arboretumlaan 4, Wageningen, 6703 BD, The Netherlands
*
*Correspondence Fax: +31 317 484740, Email: henk.hilhorst@wur.nl
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Abstract

The desiccation sensitivity of seeds of Inga vera Willd. subsp. affinis, a recalcitrant-seeded tree from Brazil, was analysed, focusing on water relations and cell-cycle aspects, including DNA content and the microtubular cytoskeleton. Seeds were collected at four developmental stages, dried to different moisture contents (MCs), assessed regarding water activity and set to germinate. Samples of fresh (non-dried) developing and mature seeds were used for assessment of DNA content by flow cytometry. Immunohistochemical detection of microtubules (MTs) was done in mature seeds at different MCs. Slight desiccation of immature seeds increased germination, but further drying resulted in a quick decline of germinability. During seed development the desiccation sensitivity decreased slightly, but DNA content of the embryonic axis cells remained constant, suggesting no relation between those two parameters. Embryonic axis cells of mature seeds showed abundant cortical microtubule arrays, which were not affected by mild desiccation, but broken down by further drying. It appeared that, upon rehydration, damaged cells were not able to reconstitute the microtubular cytoskeleton. The failure of germination of Inga vera seeds after drying could not be attributed to cellular damage to DNA synthesis and mitosis, since the radicle protruded by means of cell elongation, without a need for cell division. However, the breakdown of MTs during desiccation, and their subsequent inability to reassemble upon rehydration, may be related to the decreased germination, since MTs are required for cell elongation.

Keywords

cell cycleDNA contentInga veramicrotubulesrecalcitrant seedseed desiccation
Type
Research Article
Information
Seed Science Research , Volume 14 , Issue 2 , May 2004 , pp. 165 - 178
Copyright
Copyright © Cambridge University Press 2004

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