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Characterization and diversity of seed endophytic bacteria of the endemic holoparasitic plant Cistanche armena (Orobanchaceae) from a semi-desert area in Armenia

Published online by Cambridge University Press:  18 October 2022

Kristine Petrosyan Open the ORCID record for Kristine Petrosyan [Opens in a new window] ,
Sofie Thijs Open the ORCID record for Sofie Thijs [Opens in a new window] ,
Renata Piwowarczyk Open the ORCID record for Renata Piwowarczyk [Opens in a new window] ,
Karolina Ruraż Open the ORCID record for Karolina Ruraż [Opens in a new window] ,
Jaco Vangronsveld Open the ORCID record for Jaco Vangronsveld [Opens in a new window]  and
Wiesław Kaca Open the ORCID record for Wiesław Kaca [Opens in a new window]
Kristine Petrosyan*
Affiliation:
Department of Microbiology, Institute of Biology, Jan Kochanowski University, Uniwersytecka 7, Kielce 25-406, Poland Centre for Environmental Sciences, Environmental Biology Research Group, Hasselt University, Agoralaan Building D, Diepenbeek 3590, Belgium
Sofie Thijs
Affiliation:
Centre for Environmental Sciences, Environmental Biology Research Group, Hasselt University, Agoralaan Building D, Diepenbeek 3590, Belgium
Renata Piwowarczyk
Affiliation:
Center for Research and Conservation of Biodiversity, Department of Environmental Biology, Institute of Biology, Jan Kochanowski University, Uniwersytecka 7, Kielce 25-406, Poland
Karolina Ruraż
Affiliation:
Center for Research and Conservation of Biodiversity, Department of Environmental Biology, Institute of Biology, Jan Kochanowski University, Uniwersytecka 7, Kielce 25-406, Poland
Jaco Vangronsveld
Affiliation:
Centre for Environmental Sciences, Environmental Biology Research Group, Hasselt University, Agoralaan Building D, Diepenbeek 3590, Belgium Institute of Biological Sciences, Department of Plant Physiology and Biophysics, Faculty of Biology and Biotechnology, Maria Curie-Skłodowska University, 19 Akademicka, Lublin 20-033, Poland
Wiesław Kaca
Affiliation:
Department of Microbiology, Institute of Biology, Jan Kochanowski University, Uniwersytecka 7, Kielce 25-406, Poland
*
*Author for Correspondence: Kristine Petrosyan, E-mail: kristine.petrosyan@phd.ujk.edu.pl
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Abstract

We explored the seed-associated bacterial endophytic microbiome in seeds of the endemic holoparasitic species Cistanche armena from a saline and arid habitat in Armenia. A combination of culture-dependent and molecular techniques was employed for identifying the seed endomicrobiome (culturable and unculturable). From surface-sterilized seeds, 10 phyla, comprising 256 endophytic bacterial genera, were identified. Of the culturable strains, we also investigated the plant growth-promoting (PGP) traits. Most of the isolates were spore forming, halotolerant and alkaliphile Bacillus spp., indicating that the endophytic bacteria of C. armena seeds own traits related to the natural habitat of their host plant. Our results confirm that Bacillus species are common and dominated endophytes from plants growing on saline and arid soils. Pantoea spp. and Stenotrophomonas spp. are more favourable PGP endophytes in seeds of C. armena. The PGP traits of these bacteria, such as production of indole, a precursor of auxin, ACC-deaminase and organic acids have the potential to improve the tolerance of their host plants against the abiotic stresses present in their natural habitat. To the best of our knowledge, this is the first report concerning bacterial seed endophytes of the C. armena.

Keywords

abiotic stressBacillusdust seedsPantoeaparasitic plantsPGP traitsseed microbiome
Type
Research Paper
Information
Seed Science Research , Volume 32 , Issue 4 , December 2022 , pp. 264 - 273
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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