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Changes in the sensitivity of parasitic weed seeds to germination stimulants

  • Radoslava Matusova (a1) (a2), Tom van Mourik (a3) and Harro J. Bouwmeester (a1)

Abstract

The effects of preconditioning temperature and preconditioning period on the sensitivity of parasitic weed seeds to the synthetic germination stimulant GR24 were studied under laboratory and field conditions. The temperature during preconditioning of Orobanche cumana and Striga hermonthica seeds strongly affected the responsiveness of the seeds to the applied germination stimulant. Preconditioning at an optimal temperature (21°C for O. cumana and 30°C for S. hermonthica) rapidly released dormancy and increased the sensitivity to GR24 by several orders of magnitude. After reaching maximum sensitivity, prolonged preconditioning rapidly induced secondary dormancy, i.e. decreased sensitivity of O. cumana and S. hermonthica to GR24. The rapid change in sensitivity of preconditioned seeds to germination stimulants during prolonged preconditioning was particularly visible at low concentrations of GR24. GR24 at higher concentrations (0.1 and 1 mg l1) usually induced high germination of both species, regardless of the preconditioning period. The striking similarities between the response of parasitic weed seeds to GR24, described here, and results in the literature on non-parasitic wild plant seeds are discussed. Our results show that parasitic weed seeds are highly sensitive to the germination stimulant for a short period of time only, and then enter into secondary dormancy relatively quickly. The similar germination pattern of S. hermonthica seeds preconditioned for prolonged periods of time under laboratory and field conditions suggests that the mechanism observed is of ecological significance.

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Corresponding author

*Correspondence Fax: +31 317 418094, Email:, harro.bouwmeester@wur.nl

References

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Akhtouch, B., Munoz-Ruz, J., Melero-Vara, J., Fernandez-Martinez, J. and Dominguez, J. (2002) Inheritance of resistance to race F of broomrape in sunflower lines of different origins. Plant Breeding 121, 266268.
Babiker, A.G.T., Ma, Y.Q., Sugimoto, Y. and Inanaga, S. (2000) Conditioning period, CO 2 and GR24 influence ethylene biosynthesis and germination of Striga hermonthica. Physiologia Plantarum 109, 7580.
Baskin, C.C. and Baskin, J.M. (1998) Seeds. Ecology, biogeography, and evolution of dormancy and germination. San Diego, Academic Press.
Bouwmeester, H.J., Matusova, R., Sun, Z.K. and Beale, M.H. (2003) Secondary metabolite signalling in host–parasitic plant interactions. Current Opinion in Plant Biology 6, 358364.
Brady, S.M. and McCourt, P. (2003) Hormone cross-talk in seed dormancy. Journal of Plant Growth Regulation 22, 2531.
Chandler, P.M. and Robertson, M. (1999) Gibberellin dose–response curves and the characterization of dwarf mutants of barley. Plant Physiology 120, 623632.
Derkx, M.P.M. and Karssen, C.M. (1993a) Changing sensitivity to light and nitrate but not to gibberellins regulates seasonal dormancy patterns in Sisymbrium officinale seeds. Plant, Cell and Environment 16, 469479.
Derkx, M.P.M. and Karssen, C.M. (1993b) Effects of light and temperature on seed dormancy and gibberellin-stimulated germination in Arabidopsis thaliana – studies with gibberellin-deficient and gibberellin-insensitive mutants. Physiologia Plantarum 89, 360368.
Derkx, M.P.M. and Karssen, C.M. (1994) Are seasonal dormancy patterns in Arabidopsis thaliana r egulated by changes in seed sensitivity to light, nitrate and gibberellin?. Annals of Botany 73, 129136.
Eizenberg, H., Plakhine, D., Hershenhorn, J., Kleifeld, Y. and Rubin, B. (2003) Resistance to broomrape ( Orobanche spp.) in sunflower ( Helianthus annuus L.) is temperature dependent. Journal of Experimental Botany 54, 13051311.
Firn, R.D. (1986) Growth substance sensitivity: The need for clearer ideas, precise terms and purposeful experiments. Physiologia Plantarum 67, 267272.
Hauck, C., Muller, S. and Schildknecht, H. (1992) A germination stimulant for parasitic flowering plants from Sorghum bicolor, a genuine host plant. Journal of Plant Physiology 139, 474478.
Hilhorst, H.W.M. (1993) New aspects of seed dormancy. 571579. in Come, D.;, Corbineau, F. (Eds) Proceedings of the fourth international workshop on seeds: Basic and applied aspects of seed biology, Vol. 2, Paris ASFIS.
Hilhorst, H.W.M. and Karssen, C.M. (1988) Dual effect of light on the gibberellin- and nitrate-stimulated seed germination of Sisymbrium officinale and Arabidopsis thaliana. Plant Physiology 86, 591597.
Hilhorst, H.W.M., Smitt, A.I. and Karssen, C.M. (1986) Gibberellin-biosynthesis and -sensitivity mediated stimulation of seed germination of Sisymbrium officinale by red light and nitrate. Physiologia Plantarum 67, 285290.
Hilhorst, H.W.M., Derkx, M.P.M. and Karssen, C.M. (1996) An integrating model for seed dormancy cycling: characterization of reversible sensitivity. pp. 341360. in Lang, G.A. (Ed.) Plant dormancy: Physiology, biochemistry and molecular biology. Wallingford, CAB International.
Hsiao, A.I., Worsham, A.D. and Moreland, D.E. (1988) Effects of temperature and dl-strigol on seed conditioning and germination of witchweed ( Striga asiatica ). Annals of Botany 61, 6572.
Ish-Shalom-Gordon, N., Jacobsohn, R. and Cohen, Y. (1994) Seasonal fluctuation in sunflower's resistance to Orobanche cumana. 351355. in Pieterse, A.H.;, Verkleij, J.A.C.;, ter Borg, S.J.Biology and management of Orobanche. Amsterdam, Royal Tropical Institute.
Joel, D.M. (2000) The long-term approach to parasitic weeds control: manipulation of specific developmental mechanisms of the parasite. Crop Protection 19, 753758.
Joel, D.M., Steffens, J.C. and Matthews, D.M. (1995) Germination of weedy root parasites. 567597. in Kigel, J.;, Galili, G. (Eds.) Seed development and germination. New York, Marcel Dekker.
Kebreab, E. and Murdoch, A.J. (1999) A quantitative model for loss of primary dormancy and induction of secondary dormancy in imbibed seeds of Orobanche spp. Journal of Experimental Botany 50, 211219.
Logan, C.D. and Stewart, G.R. (1992) Germination of the seeds of parasitic angiosperms. Seed Science Research 2, 179190.
López-Granados, F. and Garcia-Torres, L. (1999) Longevity of crenate broomrape ( Orobanche crenata ) seed under soil and laboratory conditions. Weed Science 47, 161166.
Lovegrove, A., Barratt, D.H.P., Beale, M.H. and Hooley, R. (1998) Gibberellin-photoaffinity labelling of two polypeptides in plant plasma membranes. Plant Journal 15, 311320.
Mayer, A.M. Bar and Nun, N. (1997) Germination of Orobanche seeds: Some aspects of metabolism during preconditioning. pp. 633639. in Ellis, R.H.;, Black, M.;, Murdoch, A.J.;, Hong, T.D. (Eds) Basic and applied aspects of seed biology. Dordrecht, Kluwer Academic Publishers.
Musselman, L.J. (1994) Taxonomy and spread of Orobanche. pp. 2735. in Pieterse, A.H.;, Verkleij, J.A.C.;, ter Borg, S.J. (Eds) Biology and management of Orobanche. Amsterdam Royal Tropical Institute
Parker, C. and Riches, C.R. (1993) Parasitic weeds of the world: Biology and control. Wallingford, CAB International.
Press, M.C., Scholes, J.D. and Riches, C.R. (2001) Current status and future prospects for management of parasitic weeds ( Striga and Orobanche ). pp.7188. in Riches, C.R. (Ed.) The world's worst weeds. Farnham, UK, British Crop Protection Council.
Reid, D.C. and Parker, C. (1979) Germination requirements of Striga species. 202210. in Musselman, L.J.;, Worsham, A.D.;, Eplee, R.E. (Eds) Proceedings, second international symposium on parasitic weeds. Raleigh, North Carolina State University.
Reizelman Lucassen, A. (2003) Synthesis and function of germination stimulants for seeds of the parasitic weeds Striga and Orobanche spp. PhD thesis, Katholieke Universiteit Nijmegen, Nijmegen.
Siame, B.A., Weerasuriya, Y., Wood, K., Ejeta, G. and Butler, L.G. (1993) Isolation of strigol, a germination stimulant for Striga asiatica, from host plants. Journal of Agricultural and Food Chemistry 41, 14861491.
Steadman, K.J. (2004) Dormancy release during hydrated storage in Lolium rigidum seeds is dependent on temperature, light quality, and hydration status. Journal of Experimental Botany 55, 929937.
Sugimoto, Y., Ali, A.M., Yabuta, S., Kinoshita, H., Inanaga, S. and Itai, A. (2003) Germination strategy of Striga hermonthica involves regulation of ethylene biosynthesis. Physiologia Plantarum 119, 137145.
Sukno, S., Fernandez-Martinez, J.M. and Melero-Vara, J.M. (2001) Temperature effect on the disease reactions of sunflower to infection by Orobanche cumana. Plant Disease 85, 553556.
Takeuchi, Y., Omigawa, Y., Ogasawara, M., Yoneyama, K., Konnai, M. and Worsham, A.D. (1995) Effects of brassinosteroids on conditioning and germination of clover broomrape ( Orobanche minor ) seeds. Plant Growth Regulation 16, 153160.
Totterdell, S. and Roberts, E.H. (1979) Effects of low temperatures on the loss of innate dormancy and the development of induced dormancy in seeds of Rumex obtusifolius L. and Rumex crispus L. Plant, Cell and Environment 2, 131137.
Vallance, K.B. (1950) Studies on the germination of the seeds of Striga hermonthica 1: The influence of moisture treatment, stimulant-dilution and after-ripening on germination. Annals of Botany 14, 347363.
Van Hezewijk, M.J., Van Beem, A.P., Verkleij, J.A.C. and Pieterse, A.H. (1993) Germination of Orobanche crenata seeds, as influenced by conditioning temperature and period. Canadian Journal of Botany 71, 786792.
Van Hezewijk, M.J., Linke, K.H., López-Granados, F., Al-Menoufi, O.A., Garcia-Torres, L., Saxena, M.C., Verkleij, J.A.C. and Pieterse, A.H. (1994) Seasonal changes in germination response of buried seeds of Orobanche crenata Forsk. Weed Research 34, 369376.
Vleeshouwers, L.M., Bouwmeester, H.J. and Karssen, C.M. (1995) Redefining seed dormancy: an attempt to integrate physiology and ecology. Journal of Ecology 83, 10311037.
Weyers, J.D.B., Paterson, N.W. and A' Brook, R. (1987) Towards a quantitative definition of plant hormone sensitivity. Plant, Cell and Environment 10, 110.
Wigchert, S.C.M. and Zwanenburg, B. (1999) A critical account on the inception of Striga seed germination. Journal of Agricultural and Food Chemistry 47, 13201325.
Yokota, T., Sakai, H., Okuno, K., Yoneyama, K. and Takeuchi, Y. (1998) Alectrol and orobanchol, germination stimulants for Orobanche minor, from its host red clover. Phytochemistry 49, 19671973.

Keywords

Changes in the sensitivity of parasitic weed seeds to germination stimulants

  • Radoslava Matusova (a1) (a2), Tom van Mourik (a3) and Harro J. Bouwmeester (a1)

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