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Caffeine content variation in single green Arabica coffee seeds

Published online by Cambridge University Press:  02 June 2010

Paulo Mazzafera
Affiliation:
Department of Plant Biology, Institute of Biology, State University of Campinas, CP 6109, 13083-970Campinas, SP, Brazil
Maria Bernadete Silvarolla
Affiliation:
Coffee Research Center Alcides Carvalho, Agronomic Institute of Campinas, CP 28, 13012-970Campinas, SP, Brazil
Corresponding
E-mail address:

Abstract

Coffea arabica L. is highly homozygotic and caffeine content in most cultivars is reported to be similar (approximately 1%). The caffeine content was analysed using individual seeds of cultivars Catuaí Vermelho, Mundo Novo and Bourbon Vermelho, and varieties Mokka and Laurina of C. arabica. Seeds were cut transversely in half, a thin slice from each was extracted in a mixture of chloroform and methanol, and caffeine was measured by high performance liquid chromatography. The extraction procedure was validated by comparison with two conventional methods less suited for analysing large numbers of seeds. The results revealed an unexpectedly large variation [Catuaí Vermelho: 3.72–25.9 mg g− 1 (seeds); Mundo Novo: 5.06–18.59 mg g− 1; Bourbon Vermelho: 6.76–16.59 mg g− 1] even in types naturally low in caffeine such as Laurina (1.17–9.97 mg g− 1) and Mokka (2.01–9.64 mg g− 1). The mean values for all cultivars were always in the range of the caffeine content found in the literature. Several studies showed that C. arabica has approximately 10% cross-pollination, but the variation observed in caffeine content in individual seeds was too large to be explained by cross-pollination.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2010

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References

Ashihara, H. and Suzuki, T. (2004) Distribution and biosynthesis of caffeine in plants. Frontiers in Bioscience 9, 18641876.CrossRefGoogle ScholarPubMed
Ashihara, H., Sano, H. and Crozier, A. (2008) Caffeine and related purine alkaloids: biosynthesis, catabolism, function and genetic engineering. Phytochemistry 69, 8331076.CrossRefGoogle ScholarPubMed
Barre, P., Akaffou, S., Louarn, J., Charrier, A., Hamon, S. and Noirot, M. (1998) Inheritance of caffeine and heteroside contents in an interspecific cross between a cultivated coffee species Coffea liberica var dewevrei and a wild species caffeine-free C. pseudozanguebariae. Theoretical and Applied Genetics 96, 306311.CrossRefGoogle Scholar
Baumann, T.W., Sondahl, M.R., Waldahauser, S.S.M. and Kretschmar, J.A. (1998) Non-destructive analysis of natural variability in bean caffeine content of Laurina coffee. Phytochemistry 49, 15691573.CrossRefGoogle ScholarPubMed
Carvalho, A. (1988) Principles and practices of coffee plant breeding for productivity and quality factors: Coffea arabica. pp. 129165in Clarke, R.J.; Macrae, R. (Eds) Coffee: Agronomy. London, Elsevier Applied Science.Google Scholar
Carvalho, A., Medina Filho, H.P., Fazuoli, L.C., Guerreiro Filho, O. and Lima, M.M.A (1991) Aspectos genéticos do cafeeiro. Revista Brasileira de Genética 14, 135183.Google Scholar
Clifford, M.N. (1985) Chemical and physical aspects of green coffee and coffee products. pp. 305374in Clifford, M.N.; Wilson, K.C. (Eds) Coffee: Botany, biochemistry and production of beans and beverage. Westport, Connecticut, USA, AVI Publishing.CrossRefGoogle Scholar
Coffee Board of India (2004) Coffee and health. Bangalore, Ministry of Commerce and Industry, Government of India.Google Scholar
Katz, S.N. (1985) Decaffeination of coffee. pp. 5971in Clarke, R.J.; Macrae, R. (Eds) Coffee: Vol. 2, Technology. London, Elsevier Applied Science.Google Scholar
Mazzafera, P. (2004) Catabolism of caffeine in plants and microorganisms. Frontiers in Bioscience 9, 13481359.CrossRefGoogle ScholarPubMed
Mazzafera, P. and Carvalho, A. (1992) Breeding for low seed caffeine content of coffee (Coffea L.) by interspecific hybridization. Euphytica 59, 5560.Google Scholar
Mazzafera, P., Carvalho, A., Fazuoli, L.C. and Medina Filho, H.P. (1992) Variabilidade do teor de cafeína em sementes de café. Turrialba 42, 231237.Google Scholar
Mazzafera, P., Silvarolla, M.B., Lima, M.M.A. and Medina Filho, H.P. (1997) Caffeine content of diploid coffee species. Ciência e Cultura 49, 216218.Google Scholar
Monaco, L.C., Melo, M. and Carvalho, A. (1975) Controle genético da síntese da cafeína. Ciência e Cultura 27, 255.Google Scholar
Nawrot, P., Jordan, S., Eastwood, J., Rotstein, J., Hugenholtz, A. and Feeley, M. (2003) Effects of caffeine on human health. Food Additives Contaminants 20, 130.CrossRefGoogle ScholarPubMed
Ogita, S., Uefuji, H., Yamaguchi, Y., Koizumi, N. and Sano, H. (2003) Producing decaffeinated coffee plants. Nature 423, 823.CrossRefGoogle ScholarPubMed
Ogita, S., Uefuji, H., Morimoto, M. and Sano, H. (2004) Application of RNAi to confirm theobromine as the major intermediate for caffeine biosynthesis in coffee plants with potential for construction of decaffeinated varieties. Plant Molecular Biology 54, 931941.CrossRefGoogle ScholarPubMed
Priolli, R.H.G., Mazzafera, P., Siqueira, W.J., Möller, M., Zucchi, M.I., Ramos, L.C.S. and Colombo, C.A. (2008) Caffeine inheritance in interspecific hybrids of Coffea arabica × Coffea canephora (Gentianales, Rubiaceae). Genetics and Molecular Biology 31, 498504.CrossRefGoogle Scholar
Ramalakshmi, K.andRaghavan, B. (1999) Caffeine in coffee: its removal – why and how? Critical Reviews in Food Science and Nutrition 39, 441456.CrossRefGoogle Scholar
Silvarolla, M.B., Mazzafera, P. and Lima, M.M.A. (2000) Caffeine content of Ethiopian Coffea arabica beans. Genetics and Molecular Biology 23, 213215.CrossRefGoogle Scholar
Silvarolla, M.B., Mazzafera, P. and Fazuoli, L.C. (2004) A naturally decaffeinated arabica coffee. Nature 429, 826.CrossRefGoogle ScholarPubMed
Vincent, J.C. (1985) Green coffee processing. pp. 133in Clarke, R.J.; Macrae, R. (Eds) Coffee: Vol. 2, Technology. London, Elsevier Applied Science.Google Scholar

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