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Identification and punctate nuclear localization of a novel noncoding RNA, Ks-1, from the honeybee brain

Published online by Cambridge University Press:  20 August 2002

MIYUKI SAWATA ,
DAISUKE YOSHINO ,
HIDEAKI TAKEUCHI ,
AZUSA KAMIKOUCHI ,
KAZUAKI OHASHI  and
TAKEO KUBO
MIYUKI SAWATA
Affiliation:
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
DAISUKE YOSHINO
Affiliation:
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
HIDEAKI TAKEUCHI
Affiliation:
Bio-oriented Technology Research Advancement Institution (BRAIN), 3-18-19, Toranomon, Minato-ku, Tokyo 105-0001, Japan
AZUSA KAMIKOUCHI
Affiliation:
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
KAZUAKI OHASHI
Affiliation:
Graduate School of Pharmaceutical Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan
TAKEO KUBO
Affiliation:
Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan Bio-oriented Technology Research Advancement Institution (BRAIN), 3-18-19, Toranomon, Minato-ku, Tokyo 105-0001, Japan
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Abstract

We identified a novel gene, Ks-1, which is expressed preferentially in the small-type Kenyon cells of the honeybee brain. This gene is also expressed in some of the large soma neurons in the brain and in the suboesophageal ganglion. Reverse transcription–polymerase chain reaction experiments indicated that Ks-1 transcripts are enriched in the honeybee brain. cDNA cloning revealed that the consensus Ks-1 cDNA is over 17 kbp and contains no significant open reading frames. Furthermore, fluorescent in situ hybridization revealed that Ks-1 transcripts are located in the nuclei of the neural cells, accumulating in some scattered spots. These findings demonstrate that Ks-1 encodes a novel class of noncoding nuclear RNA and is possibly involved in the regulation of neural functions.

Keywords

Kenyon cellsmushroom bodiesneuronnuclear RNA
Type
Research Article
Information
RNA , Volume 8 , Issue 6 , June 2002 , pp. 772 - 785
Copyright
© 2002 RNA Society

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