Skip to main content Accessibility help
×
Home

Characterization of U6 snRNA–protein interactions

  • VALERIE P.I. VIDAL (a1) (a2), LOREDANA VERDONE (a1), ANDREW E. MAYES (a1) (a3) and JEAN D. BEGGS (a1)

Abstract

Through a combination of in vitro snRNP reconstitution, photocross-linking and immunoprecipitation techniques, we have investigated the interaction of proteins with the spliceosomal U6 snRNA in U6 snRNPs, U4/U6 di-snRNPs and U4/U6.U5 tri-snRNPs. Of the seven Lsm (Sm-like) proteins that associate specifically with this spliceosomal snRNA, three were shown to contact the RNA directly, and to maintain contact as the U6 RNA is incorporated into tri-snRNPs. In tri-snRNPs, the U5 snRNP protein Prp8 contacts position 54 of U6, which is in the conserved region that contributes to the formation of the catalytic core of the spliceosome. Other tri-snRNP-specific contacts were also detected, indicating the dynamic nature of protein interactions with this important snRNA. The uridine-rich extreme 3′ end of U6 RNA was shown to be essential but not sufficient for the association of the Lsm proteins. Interestingly, the Lsm proteins associate efficiently with the 3′ half of U6, which contains the 3′ stem-loop and uridine-rich 3′ end, suggesting that the Lsm and Sm proteins may recognize similar features in RNAs.

Copyright

Corresponding author

Reprint requests to: Jean D. Beggs, Institute of Cell and Molecular Biology, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, United Kingdom; e-mail: jbeggs@ed.ac.uk.

Keywords

Characterization of U6 snRNA–protein interactions

  • VALERIE P.I. VIDAL (a1) (a2), LOREDANA VERDONE (a1), ANDREW E. MAYES (a1) (a3) and JEAN D. BEGGS (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed