Skip to main content Accessibility help
×
Home
Hostname: page-component-8bbf57454-5qtdt Total loading time: 0.291 Render date: 2022-01-21T11:12:49.449Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": true, "newCiteModal": false, "newCitedByModal": true, "newEcommerce": true, "newUsageEvents": true }
RNA RNA

Article contents

Refolding of rRNA exons enhances dissociation of the Tetrahymena intron

Published online by Cambridge University Press:  01 September 2000

YONG CAO
Affiliation:
Molecular and Cell Biology Program, University of Maryland, College Park, Maryland 20742-2021, USA Present address: Department of Microbiology and Immunology, Virginia Commonwealth University Medical College, P.O. Box 980678, Richmond, Virginia 23298-0678, USA.
SARAH A. WOODSON
Affiliation:
Molecular and Cell Biology Program, University of Maryland, College Park, Maryland 20742-2021, USA Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742-2021, USA Present address: T.C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, Maryland 21218, USA.
Get access

Abstract

Self-splicing of the Tetrahymena pre-rRNA is inhibited by a conserved rRNA hairpin P(−1) upstream of the 5′ splice site. P(−1) inhibits self-splicing by competing with formation of the P1 splice site helix. Here we show that the P(−1) hairpin also enhances dissociation of the spliced products, which was monitored by native gel electrophoresis. Mutations that stabilize the rRNA hairpin increase the rate of dissociation approximately 10-fold, from 0.5 min−1 for the wild-type RNA to ∼4 min−1 at 30 °C. Conversely, mutations or oligonucleotides that inhibit refolding of the exons and that stabilize the P1 helix decrease the rate of product release. The results suggest that refolding of products can be used to stimulate the turnover of ribozyme-catalyzed reactions. In the pre-rRNA, this conformational change helps shift the equilibrium of self-splicing toward the mature rRNA.

Type
Research Article
Information
RNA , Volume 6 , Issue 9 , September 2000 , pp. 1248 - 1256
Copyright
2000 RNA Society

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Refolding of rRNA exons enhances dissociation of the Tetrahymena intron
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Refolding of rRNA exons enhances dissociation of the Tetrahymena intron
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Refolding of rRNA exons enhances dissociation of the Tetrahymena intron
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *