Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
Home
  • Home
Hostname: page-component-559fc8cf4f-67gxp Total loading time: 0.204 Render date: 2021-03-06T09:11:53.737Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": false, "newCiteModal": false, "newCitedByModal": true }
EnglishFrançais
RNA RNA

Article contents

Features of the autonomous function of the translational enhancer domain of satellite tobacco necrosis virus

Published online by Cambridge University Press:  01 November 1998

FRANK MEULEWAETER ,
MARC VAN MONTAGU  and
MARC CORNELISSEN
FRANK MEULEWAETER
Affiliation:
Plant Genetic Systems N.V., J. Plateaustraat 22, 9000 Gent, Belgium Laboratorium voor Genetica, Universiteit Gent, K. L. Ledeganckstraat 35, 9000 Gent, Belgium
MARC VAN MONTAGU
Affiliation:
Laboratorium voor Genetica, Universiteit Gent, K. L. Ledeganckstraat 35, 9000 Gent, Belgium
MARC CORNELISSEN
Affiliation:
Plant Genetic Systems N.V., J. Plateaustraat 22, 9000 Gent, Belgium
Get access

Abstract

The RNA of satellite tobacco necrosis virus (STNV) is a monocistronic messenger that lacks both a cap and a poly(A) tail. Translation of STNV RNA in vitro is promoted by a 120-nt translational enhancer domain (TED) in the 3′-untranslated region. TED also stimulates translation of heterologous mRNAs. In this study, we show that TED stimulates translation of a cat mRNA by increasing translation efficiency to the level of capped mRNA. This stimulatory activity is not impaired by translation through TED. TED stimulates translation efficiency from different positions within the mRNA, varying from the 5′ end to 940 nt downstream of the coding region. Duplication of TED has an additive effect on translation stimulation only when located at both ends of the mRNA. On dicistronic RNAs, TED stimulates translation of both cistrons to the same extent. These data suggest that TED acts primarily by recruiting the translational machinery to the RNA.

Keywords

3′-UTR cap-independent translation plant virus wheat germ
Type
Research Article
Information
RNA , Volume 4 , Issue 11 , November 1998 , pp. 1347 - 1356
Copyright
© 1998 RNA Society

Access options

Get access to the full version of this content by using one of the access options below.
If you should have access and can't see this content please contact technical support.

Full text views

Full text views reflects PDF downloads, PDFs sent to Google Drive, Dropbox and Kindle and HTML full text views.

Total number of HTML views: 0
Total number of PDF views: 5 *
View data table for this chart

* Views captured on Cambridge Core between September 2016 - 6th March 2021. This data will be updated every 24 hours.

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Features of the autonomous function of the translational enhancer domain of satellite tobacco necrosis virus
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Features of the autonomous function of the translational enhancer domain of satellite tobacco necrosis virus
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Features of the autonomous function of the translational enhancer domain of satellite tobacco necrosis virus
Available formats
×
×

Reply to: Submit a response

Your details

Conflicting interests

Do you have any conflicting interests? *