Hostname: page-component-7dc689bd49-kfntg Total loading time: 0 Render date: 2023-03-20T14:07:26.128Z Has data issue: true Feature Flags: { "useRatesEcommerce": false } hasContentIssue true
  • English

An engineered class I transfer RNA with a class II tertiary fold

Published online by Cambridge University Press:  01 March 1999

T. AMAR NISSAN ,
BRYANT OLIPHANT  and
JOHN J. PERONA
T. AMAR NISSAN
Affiliation:
Department of Chemistry, and Interdepartmental Program in Biochemistry and Molecular Biology, University of California at Santa Barbara, Santa Barbara, California 93106-9510, USA
BRYANT OLIPHANT
Affiliation:
Department of Chemistry, and Interdepartmental Program in Biochemistry and Molecular Biology, University of California at Santa Barbara, Santa Barbara, California 93106-9510, USA
JOHN J. PERONA
Affiliation:
Department of Chemistry, and Interdepartmental Program in Biochemistry and Molecular Biology, University of California at Santa Barbara, Santa Barbara, California 93106-9510, USA
Get access

Abstract

Structure-based engineering of the tertiary fold of Escherichia coli tRNA2Gln has enabled conversion of this transfer RNA to a class II structure while retaining recognition properties of a class I glutamine tRNA. The new tRNA possesses the 20-nt variable stem-loop of Thermus thermophilus tRNASer. Enlargement of the D-loop appears essential to maintaining a stable tertiary structure in this species, while rearrangement of a base triple in the augmented D-stem is critical for efficient glutaminylation. These data provide new insight into structural determinants distinguishing the class I and class II tRNA folds, and demonstrate a marked sensitivity of glutaminyl-tRNA synthetase to alteration of tRNA tertiary structure.

Keywords

aminoacyl-tRNA synthetasesextra armRNA engineeringRNA structuretRNA identity
Type
Research Article
Information
RNA , Volume 5 , Issue 3 , March 1999 , pp. 434 - 445
Copyright
1999 RNA Society

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)