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Diagnostic significance of sperm-zona pellucida interaction

  • Sergio Oehninger (a1)

Extract

Spermatozoa binding to the zona pellucida is an early, critical event leading to fertilization and early pre-embryo development. Fertilization involves a complex and orderly sequence of events that is completed at syngamy, which is defined as the union of the two sets of haploid chromosomes to form a new diploid fertilized ovum (zygote). In order to be able to fertilize an oocyte, spermatozoa need to undergo a process called ‘capacitation’, which is usually defined as a series of changes that renders the sperm cells capable of undergoing the acrosome reaction. This process that naturally occurs within the female genital tract is possible under in vitro conditions. However, capacitation is not the only process spermatozoa must undergo to fertilize the oocytes successfully. To fertilize an oocyte, spermatozoa must also be at least highly motile, as well as being capable of undergoing the acrosome reaction timely, penetrating through the oocyte investments and fusing with the oocyte plasma membrane properly.

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Corresponding author

Sergio Oehninger, Assistant Professor, The Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, 601 Colley Avenue, Sixth Floor, Norfolk, Virginia 23507, USA.

References

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1 Yanagimachi, R. Mammalian fertilization. In: Knobil E, Neill J eds. New York: Raven Press, 1988: 135–85.
2 Austin, CR. Capacitation and the release of hyaluronidase from spermatozoa. J Reprod Fertil 1960; 3: 310–11.
3 Austin, CR. Capacitation of spermatozoa. Int J Fertil 1967; 12:2531.
4 Bedford, JM. Sperm capacitation and fertilization in mammals. Biol Reprod 1970; 2(suppl) 128–58.
5 Yanagimachi, R, Chang, MC. Fertilization of hamster eggs in vitro. Nature 1963; 200: 281–82.
6 Yanagimachi, R. Mechanisms of fertilization in mammals. In: Mastroianni, L, Biggers, JD eds. Fertilization and embryonic development in vitro. New York: Plenum Press, 1981: 81187.
7 Acosta, AA, Oehninger, S, Morshedi, M, Swanson, RJ, Scott, R, Irianni, F. Assisted reproduction and treatment of the male factor. Obstet Gynecol Surv 1989; 44: 118.
8 Irianni, F, Acosta, AA, Oehninger, S, Acosta, R. Therapeutic insemination – controversial treatment for infertility. Arch Androl 1990; 25: 147–67.
9 Oehninger, S, Hodgen, GD. How to evaluate human spermatozoa for assisted reproduction. In: DeCherney, A ed. Assisted reproduction review. Baltimore: Williams and Wilkins, 1991: 1527.
10 Mahadevan, MM, Trounson, AO. The influence of seminal characteristics on the success rate of human in vitro fertilization. Fertil Steril 1984; 42: 400405.
11 Rodriguez-Rigau, LJ, Ayala, G, Grovert, GM et al. Relationship between the results of sperm analysis and GIFT. J Androl 1989; 10: 139–44.
12 Oehninger, S, Acosta, AA, Veeck, L et al. Recurrent failure of IVF: role of the hemizona assay in the sequential diagnosis of specific sperm-oocyte defects. Am J Obstet Gynecol 1991; 164: 1210–15.
13 Oehninger, S, Acosta, AA, Kruger, T, Veeck, LL, Flood, J, Jones, HW Jr. Failure of fertilization in in vitro fertilization: the ‘occult’ male factor. J In Vitro Fertil Embryo Transf 1988; 5: 181–87.
14 Oehninger, S, Acosta, A, Veeck, L, Simonetti, S, Muasher, SJ. Delayed fertilization during IVF and embryo transfer cycles: analysis of causes and impact on overall results. Fertil Steril 1989; 52: 991–97.
15 Jeulin, C, Feneux, D, Serres, C et al. Sperm factors related to failure of human IVF. J Reprod Fertil 1986;70:735–44.
16 Kruger, TF, Menkveld, R, Stander, FSH et al. Sperm morphologic features as a prognostic factor i. in vitro fertilization. Fertil Steril 1986; 46: 1118–23.
17 Kruger, TF, Acosta, A, Simmons, KF, Swanson, RJ, Mata, JF, Oehninger, S. Predictive value of abnormal sperm morphology in in vitro fertilization. Fertil Steril 1988; 49: 112–17.
18 Menkveld, R, Oettle, EE, Kruger, TF, Swanson, RJ, Acosta, AA, Oehninger, S. Atlas of human sperm morphology. Baltimore: Williams and Wilkins, 1991.
19 Ron-El, R, Nachum, H, Herman, A, Golan, A, Caspi, E, Soffer, Y. Delayed fertilization and poor embryonic development associated with impaired semen quality. Fertil Steril 1991; 55: 338–44.
20 Oehninger, S, Acosta, A, Morshedi, M et al. Corrective measures and pregnancy outcome in IVF in patients with severe sperm morphology abnormalities. Fertil Steril 1988; 150: 283–87.
21 Rodriguez-Rigau, LJ, Ayala, C, Steinberger, F. Relationship between pregnancy outcome and objective semen analysis in infertile couples. J Androl 1991;(suppl):47.
22 Byrd, W, Tsu, J, Wolf, DP. Kinetics of spontaneous and induced acrosomal loss in human sperm incubated under capacitating and noncapacitating conditions. Gamete Res 1989; 22: 109–22.
23 Huszar, G, Vigue, L, Corrales, M. Sperm creatine kinase activity in fertile and infertile oligospermic men. J Androl 1990; 11: 4046.
24 Burkman, LJ. Characterization of hyperactivated motility by human spermatozoa during capacitation: comparison of fertile and oligozoospermic sperm populations. Arch Androl 1984; 13: 153–58.
25 Burkman, LJ. Evaluation and enhancement of sperm function. In: Jones, HW Jr, Jones, GS, Rosenwaks, Z, Hodgen, GD eds. In vitro fertilization, Norfolk. Baltimore: Williams and Wilkins, 1986: 206–14.
26 Burkman, LJ. Hyperactivated motility of human spermatozoa during in vitro capacitation and implications for fertility. In: Gagnon, C ed. Controls of sperm motility: biological and clinical aspects Boca Raton: CRC Press, 1990: 303–29.
27 Calvo, L, Vantman, D, Banks, SM et al. Follicular fluid induced acrosome reaction distinguishes a subgroup of men with unexplained infertility not identified by semen analysis. Fertil Steril 1989; 52: 1048–54.
28 Cross, NL, Morales, P, Overstreet, JW, Hanson, FW. Induction of the acrosome reaction by the human zona pellucida. Biol Reprod 1988; 38: 235–44.
29 Tesarik, J. Comparison of acrosome reaction inducing activities of human cumulus oophorus, follicular fluid and ionophore A23187 in human sperm populations of proven fertilizing ability in vitro. J Reprod Fertil 1985; 74: 383–88.
30 Tesarik, J, Testart, J. Human sperm-egg interactions and their disorders: implications in the management of infertility. Hum Reprod 1989; 4: 729–41.
31 Mortimer, D. Current status of egg penetration assays. In: American Society of Andrology 16th Annual Meeting, Postgraduate Course, Montreal, Canada, 04, 1991: 81.
32 Oehninger, S, Alexander, NJ. Male infertility: the focus shifts to sperm manipulation. Curr Opin Obstet Gynecol 1991; 3: 182–90.
33 Skinner, SM, Dunbar, BS. Species variation in the zona pellucida. Adv Med Biol 1986; 205: 251–68.
34 Dunbar, BS, Wolgemuth, DJ. Structure and function of the mammalian zona pellucida, a unique extracellular matrix. Mod Cell Biol 1984; 3: 77111.
35 Wassarman, PM. The zona pellucida: a coat of many colors. Biol Essays 1987; 6: 161–66.
36 Sacco, AG. Development and maturation of the zona pellucida. In: Alexander, NJ, Griffin, D, Spieler, JM, Waites, G eds. Gamete interaction: prospects for immunocontraception. New York: Wiley-Liss, 1990: 259–76.
37 O'Rand, MG, Welch, JE, Fisher, SJ. Sperm membrane and zona pellucida interactions during fertilization. In: Dhindsa, DS, Bahl, OP eds. Molecular and cellular aspects of reproduction New York: Plenum Press, 1986: 131–44.
38 O'Rand, MG. Sperm-egg recognition and barriers to interspecies fertilization. Gamete Res 1988; 19: 315–27.
39 Sacco, AG. The zona pellucida: current status as candidate antigen for contraceptive vaccine development. Am J Reprod Immunol Microbiol 1987; 15: 172–30.
40 Henderson, CJ, Hulme, MJ, Aitken, RJ. Contraceptive potential of antibodies to the zona pellucida. J Reprod Fertil 1988; 83: 325–43.
41 Hodgen, GD. Physiology of follicular maturation. In: Jones, HW Jr, Jones, GS, Hodgen, GD, Rosenwaks, Z. In vitro fertilization, Norfolk. Baltimore: Williams and Wilkins, 1986: 829.
42 Wassarman, PM, Bleil, JD. The role of zona pellucida glycoproteins as regulators of sperm-egg interactions of the mouse. In: Frazier, WA, Glaser, L, Gottlieb, CI eds. Proceedings from the UCLA Symposium of Molecular Cell Biology: Cellular recognition. New York: Alan R Liss, 1982: 845–63.
43 Chamberlin, ME, Dean, J. Genomic organization of a sex specific gene: the primary receptor of the mouse zona pellucida. Dev Biol 1989; 131: 207–14.
44 Wassarman, PM. Profile of a mammalian sperm receptor. Development 1990; 108: 117.
45 Greve, JM, Wassarman, PM. Mouse egg extracellular coat is a matrix of interconnecting filaments possessing a structural repeat. J Mol Biol 1985; 181: 253–64.
46 Kinloch, RA, Ruiz-Seiler, B, Wassarman, PD. Genomic organization and polypeptide structure of zona pellucida glycoprotein hZP3, the hamster sperm receptor. Dev Biol 1990; 142: 414–21.
47 Sacco, AG, Palm, VS. Heteroimmunization with isolated pig zona pellucidae. J Reprod Fertil 1977; 51:165–68.
48 Shabanowitz, RB, O'Rand, MG. Characterization of the human zona pellucida from fertilized and unfertilized eggs. J Reprod Fertil 1988; 82: 151–61.
49 Shabanowitz, RB. Mouse antibodies to human zona pellucida: evidence that human ZP3 is strongly immunogenic and contains two distinct isomer chains. Biol Reprod 1990; 43: 260–70.
50 Wassarman, PM. The mammalian ovum. In: Knobil, E, Neill, J eds. The physiology of reproduction. New York: Raven Press, 1988: 69102.
51 Dean, J, Chamberlin, ME, Millar, S et al. Development expression of ZP3, a mouse zona pellucida gene. In: Yoshiraga, K, Mori, T eds. Development of preimplantation, embryos and their environment. New York: Alan R Liss, 1989; 2132.
52 Macek, MB, Shur, BD. Protein-carbohydrate complementarily in mammalian gamete recognition. Gamete Res 1988; 20: 93105.
53 Huang, TT, Ohzu, E, Yanagimachi, R. Evidence suggesting that L-Fucose is part of a recognition signal for sperm zona pellucida attachment in mammals. Gamete Res 1982; 5: 355–61.
54 Ahuja, KK. Carbohydrate determinants involved in mammalian fertilization. Am J Anat 1985; 179: 207–23.
55 Bleil, JD, Wassarman, PM. Autoradiographic visualization of the mouse egg's sperm receptor bound to sperm. J Cell Biol 1986; 102: 1303–71.
56 Bleil, JD, Greve, JM, Wassarman, PD. Identification of a secondary sperm receptor in the mouse egg zona pellucida: role in maintenance of binding of acrosome-reacted sperm to eggs. Dev Biol 1988; 178: 376–85.
57 Moller, CC, Wassarman, PM. Characterization of a proteinase that cleaves zona pellucida glycoprotein ZP2 following activation of mouse eggs. Dev Biol 1989; 132: 103–12.
58 Saling, PM. How the egg regulates sperm function during gamete interaction: facts and fantasies. Biol Reprod 1991; 44: 246–51.
59 Cummins, J, Yanagimachi, R. Development of ability to penetrate the cumulus oophorus by hamster spermatozoa capacitated in vitro. Gamete Res 1986; 15:187212.
60 Hanson, FW, Overstreet, JW. Interaction of human spermatozoa with cervical mucus in vivo. Am J Obstet Gynecol 1981; 140: 173–78.
61 Katz, DF, Morales, P, Samuels, SJ, Overstreet, JW. Mechanisms of filtration of morphologically abnormal human sperm by cervical mucus. Fertil Steril 1990; 54: 513–16.
62 Krzanowska, H, Lorenc, E. Influence of egg vestments on in vitro penetration of mouse eggs by misshapen spermatozoa. J Reprod Fertil 1983; 68: 5762.
63 Kot, CM, Handel, AM. Binding of morphological abnormal sperm to mouse egg zona pellucida in vitro. Gamete Res 1987; 18: 5762.
64 Brown, CR, Jones, R. Binding of zona pellucida protein to a sperm polypeptide of Mr 53,000 and identification of zona moieties involved. Development 1987; 99: 333–39.
65 Urch, VA, Pattel, H. The interaction of boar sperm proacrosin with its natural substrate, the zona pellucida, and with sulfated polysaccharides. Development 1991; 111: 1165–72.
66 Jones, R. Interaction of zona pellucida glycoproteins sulphated carbohydrates and synthetic polymers with proacrosin, the putative egg-binding protein from mammalian spermatozoa. Development 1991; 111: 1155–63.
67 Overstreet, JW, Hembree, WC. Penetration of zona pellucida of non-living human oocytes by human spermatozoa in vitro. Fertil Steril 1976; 27: 815–31.
68 Burkman, LJ, Coddington, CC, Franken, DR, Kruger, T, Rosenwaks, Z, Hodgen, GD. The hemizona assay: development of a diagnostic test for the binding of human spermatozoa to the human zona pellucida to predict fertilization potential. Fertil Steril 1988; 49: 688–93.
69 Blazak, WF, Overstreet, JW, Katz, DF, Hanson, FW. A competitive in vitro assay of human sperm fertilizing ability utilizing contrasting fluorescent sperm markers. J Androl 1982; 3: 165–71.
70 Overstreet, JW, Yanagimachi, R, Katz, DF, Hayashi, K, Hanson, FW. Penetration of human spermatozoa into the human zona pellucida and the zona-free hamster egg: a study of fertile donors and infertile patients. Fertil Steril 1980; 33: 534–42.
71 Liu, DY, Lopata, A, Leung, A, Johnston, WIAH, Gordon Baker, HW. A human sperm-zona pellucida binding test using oocytes that failed to fertilize in vitro. Fertil Steril 1988; 50: 782–88.
72 Liu, DY, Clarke, GN, Lopata, A, Johnston, WIH, Gordon Baker, HW. A sperm-zona pellucida binding test and in vitro fertilization. Fertil Steril 1989; 52: 281–87.
73 Morales, P, Vantman, D, Madaniaja, M, Gutierrez, G. Human sperm binding and penetration to the zona pellucida of cadaveric oocytes and IVF outcome. Fifteenth Annual Meeting, American Society of Andrology, Columbia SC, 04 1990: abstract no. 80.
74 Yanagimachi, R, Yanagimachi, H, Rogers, BJ. The use of zona-free animal ova as a test system for the assessment of the fertilizing capacity of human spermatozoa. Biol Reprod 1976; 15: 471–76.
75 Barros, C, Jedlicki, A, Vigil, P. The gamete membrane fusion test to assay the fertilizing ability of human spermatozoa. Hum Reprod 1988; 3: 637–44.
76 Margalioth, EJ, Navot, D, Laufer, N, Lewin, A, Rabinowitz, R, Schenker, JG. Correlation between the zona-free hamster egg sperm penetration assay and human in vitro fertilization. Fertil Steril 1986; 45: 665–70.
77 Wolf, DP, Sokoloski, JE, Quigley, MM. Correlation of human in vitro fertilization with the hamster egg bioassay. Fertil Steril 1983; 40: 5359.
78 Ausmanas, M, Tureck, RW, Blasco, L, Kopf, GS, Ribas, J, Mastroianni, L Jr. The zona-free hamster egg penetration assay as a prognostic indicator in a human in vitro fertilization program. Fertil Steril 1985; 43: 433–37.
79 Blekien, L, Bordt, J, Freischem, CW, Hano, R, Knuth, UA, Nieschlag, E. Prognostic value of the heterologous ovum penetration test for human in vitro fertilization. Int J Androl 1985; 85: 275–84.
80 Kruger, TF, Swanson, RJ, Hamilton, M et al. Abnormal sperm morphology and other semen parameters related to the outcome of the hamster oocyte human sperm penetration assay. Int J Androl 1988;11: 107–13.
81 Lamb, DJ, Johnson, AR, Bassham, BL, Lipshultz, LI. How the sperm penetration assay diagnoses infertility. Contemp Obstet Gynecol 1989; 33: 108–12.
82 Yee, B, Cummings, LM. Modification of the sperm penetration assay using human follicular fluid to minimize false negative results. Fertil Steril 1988; 50: 123–28.
83 Aitken, RJ, Ross, A, Hargreave, T, Richardson, D, Best, F. Analysis of human sperm function following exposure to the ionophore A23187. Comparison of normospermic and oligozoospermic men. J Androl 1984; 5:321–29.
84 Mortimer, D, Curtis, EF, Dravland, JE. The use of strontium-substituted media for capacitating human spermatozoa: an improved sperm preparation method for the zona-free hamster egg penetration test. Fertil Steril 1986; 46: 97103.
85 Aitken, RJ, Wang, Y-F, Liu, J, Best, F, Richardson, DW. The influence of medium composition, osmolarity and albumin content on the acrosome reaction and fertilizing capacity of human spermatozoa: development of an improved zona-free hamster egg penetration test. Int J Androl 1983; 6: 180–83.
86 World Health Organization. WHO laboratory manual for the examination of human semen and semen-cervical mucus interaction. Second edition. Cambridge: The Press Syndicate of the University of Cambridge, 1987; 5558.
87 Tesarik, J. The potential use of human zona-free eggs prepared from oocytes that failed to fertilize in vitro. Fertil Steril 1989; 52: 821–24.
88 Burkman, LJ, Coddington, CC, Franken, D, Oehninger, S, Hodgen, GD. The hemizona assay: assessment of fertilizing potential by means of human sperm binding to human zona pellucida. In: Keel, BA, Webster, BW eds. Handbook of the laboratory diagnosis and treatment of infertility. Boca Raton: CRC Press, 1990; 213–28.
89 Franken, DR, Burkman, LJ, Coddington, CC, Oehninger, S, Hodgen, GD. Human hemizona attachment assay. In: Acosta, A, Swanson, RJ, Ackerman, SB et al. eds. Baltimore: Williams and Wilkins, 1990; 355–71.
90 Hodgen, GD, Burkman, LJ, Coddington, CC et al. The hemizona assay: finding sperm that have the ‘right stuff’. J In Vitro Fertil Embryo Transf 1988; 5: 311–13.
91 Oehninger, S, Scott, R, Muasher, S, Coddington, CC, Acosta, AA, Hodgen, GD. Hemizona assay: assessment of human spermatozoa dysfunction and its correlation with IVF results. In: Proceedings from the VI World Congress in IV F and Alternative Assisted Reproduction, Jerusalem, Israel, 04 1989. New York: Plenum Press, 1989: 515–23.
92 Oehninger, S, Franken, DR, Kruger, T, Toner, JP, Acosta, AA, Hodgen, GD. Hemizona assay: sperm defect analysis, a diagnostic method for assessment of human sperm-oocyte interactions, and predictive value for fertilization outcome. Ann NY Acad Sci 1991; 626:111–24.
93 Yanagimachi, R, Lopata, A, Odom, CB, Branson, RA, Mahi, CA, Nicolson, GL. Retention of biologic characteristics of zona pellucida in highly concentrated salt solution: the use of salt-stored eggs for assessing the fertilizing capacity of spermatozoa. Fertil Steril 1979; 31: 562–74.
94 Franken, DR, Burkman, LJ, Oehninger, SC et al. Hemizona assay using salt-stored human oocytes: evaluation of zona pellucida capacity for binding human spermatozoa. Gamete Res 1989; 22: 1526.
95 Singer, SL, Lambert, H, Overstreet, JW, Hanson, FW, Yanagimachi, R. The kinetics of human sperm binding to the human zona pellucida and zona-free hamster oocyte in vitro. Gamete Res 1985; 12: 2939.
96 Franken, DR, Oosthuizen, T, Cooper, S et al. Electron microscopic evidence on the acrosomal status of bound sperm and their penetration into human hemizonae pellucida after storage in a buffered salt solution. Andrologia 1991; 23: 205208.
97 Kruger, TF, Oehninger, S, Franken, DA, Hodgen, GD. Hemizona assay: use of fresh versus salt-stored human oocytes to evaluate sperm binding potential to the zona pellucida. J In Vitro Fertil Embryo Transf 1991; 8: 154–56.
98 Coddington, CC, Franken, DR, Burkman, LJ, Oosthuizen, WT, Kruger, TF, Hodgen, GD. Functional aspects of human sperm binding to the zona pellucida using the hemizona assay. J Androl 1991; 12: 118.
99 Franken, DR, Coddington, CC, Burkman, LJ et al. Defining the valid hemizona assay: accounting for binding variability within zonae pellucidae and within semen samples from fertile males. Fertil Steril 1991; 56: 1156–61.
100 Coddington, CC. The hemizona assay (HZA) and considerations for its use in assisted reproductive technology. Sem Reprod Endocrinol 1992 (in press).
101 Oehninger, S, Scott, RT, Coddington, CC, Franken, DR, Acosta, AA, Hodgen, GD. Validation of the hemizona assay in a monkey model: influence of oocyte maturational stages. Fertil Steril 1989; 51: 881–85.
102 Oehninger, S, Veeck, LL, Franken, D, Kruger, TF, Acosta, AA, Hodgen, GD. Human preovulatory oocytes have a higher sperm-binding ability than immature oocytes under hemizona assay conditions: evidence supporting the concept of ‘zona maturation’. Fertil Steril 1991; 55: 1165–70.
103 Oehninger, S, Morshedi, M, Ertunc, H, Bocca, S, Acosta, A, Hodgen, GD. Validation of the hemizona assay in a monkey model. II. Kinetics of binding and influence of cryopreserved-thawed spermatozoa. J In Vitro Fertil Embryo Transf 1992 (in press).
104 Lopata, A, Leung, PC. The fertilizability of human oocytes at different stages of meiotic maturation. Ann NY Acad Sci 1988; 541: 324–36.
105 Oehninger, S, Franken, DR, Menkveld, R et al. Maturation/functional ultrastructure of the human zona pellucida. ARTA 1991; 2: 253–71.
106 Zuccotti, M, Yanagimachi, R, Yanagimachi, H. The ability of hamster oolemma to fuse with spermatozoa: its acquisition during oogenesis and loss after fertilization. Development 1991; 112: 143–52.
107 Mattioli, M, Barboni, B, Bacci, ML, Seren, E. Maturation of pig oocytes: observations on membrane potential. Biol Reprod 1990; 43: 318–22.
108 Chan, PJ. Developmental potential of human oocytes according to zona pellucida thickness. J In Vitro Fertil Embryo Transf 1987; 4: 237–41.
109 Mahadevan, M, Trounson, AD, Wood, C, Leeton, JF. Effect of oocyte quality and sperm characteristics on the number of spermatozoa bound to the zona pellucida of human oocytes inseminated in vitro. J In Vitro Fertil Embryo Transf 1987; 4: 223–27.
110 Fowler, RE, Grainge, C. A histochemical study of the changes occurring in the protein-carbohydrate composition of the cumulus-oocyte complex and zona pellucida in immature mice in response to gonadotropin stimulation. Histochem J 1985; 17: 1735–49.
111 Tesarik, J, Kopecny, V. Late preovulatory synthesis of proteoglycans by the human oocyte and cumulus cells and their secretion into the oocyte-cumulus-complex extracellular matrices. Histochemistry 1986; 85: 523–28.
112 Familiari, G, Nottola, SA, Micara, G, Aragona, C, Motta, PM. Is the sperm-binding capability of the zona pellucida limited to its surface structure? A scanning electron microscopic study of human IVF. J In Vitro Fertil Embryo Transf 1988; 5: 134–43.
113 Tesarik, J, Pilka, L, Traunick, P. Zona pellucida resistance to sperm penetration before the completion of human oocyte maturation. J Reprod Fertil 1988; 83: 487–95.
114 Franken, DR, Windt, ML, Kruger, T, Oehninger, S, Hodgen, GD. Comparison of sperm binding potential of uninseminated, inseminated-unfertilized, and fertilized, non-cleaved human oocytes under hemizona assay conditions. Mol Reprod Dev 1991; 30: 5661.
115 Tesarik, J. Zona pellucida penetrability of metaphase I and II human oocytes after aging and salt treatment. Fertil Steril 1990; 54: 346–47.
116 Coddington, CC, Fulgham, DC, Alexander, NJ, Johnson, D, Herr, JC, Hodgen, GD. Sperm bound to zona pellucida in hemizona assay demonstrate acrosome reaction when stained with T-6 antibody. Fertil Steril 1990; 54: 504508.
117 Fulgham, DL, Coddington, C, Johnson, D, Herr, J, Alexander, NJ, Hodgen, GD. Human sperm acrosome reaction on zona pellucida: a time course study (submitted for publication).
118 Oehninger, S, Mahony, M, Morshedi, M, Doncel, A, Swanson, RJ, Acosta, A. Specificity of sperm/zona pellucida interactions under hemizona assay conditions: assessment of tight binding and acrosomal status in interspecies experiments. Proceedings of the 47th Annual Meeting American Fertility Society, October 1991: abstract no. 85.
119 Mahoney, M, Oehninger, S, Morshedi, M, Acosta, A, Hodgen, GD. Acrosomal status of cynomolgus monkey spermatozoa tightly bound to homologous zona pellucida in the hemizona assay. Vancouver: Society for the Study of Reproduction, 1991: abstract no. 343.
120 Robertson, L, Wolf, DP, Tash, JS. Temporal changes in motility parameters related to acrosomal status: identification and characterization of populations of hyperactivated human sperm. Biol Reprod 1988; 39: 797805.
121 David, BK, Byrne, R, Hungund, B. Studies on the mechanism of capacitation. II. Evidence for lipid transfer between plasma membrane of rat sperm and serum albumin during capacitation in vitro. Biochem Biophys Acta 1979; 327: 632–34.
122 Go, KJ, Wolf, DP. Albumin mediated changes in sperm sterol content during capacitation. Biol Reprod 1985; 32: 145–53.
123 Saling, PM. Mammalian sperm interaction with extracellular matrices of the egg. Oxford Rev Reprod Biol 1989; 11: 339–80.
124 Leyton, L, Saling, P. 95 kd sperm proteins bind ZP3 and serve as tyrosine kinase substrate in response to zona binding. Cell 1989; 57: 1123–30.
125 Leyton, L, Saling, P. Evidence that aggregation of mouse sperm receptors by ZP3 triggers the acrosome reaction. J Cell Biol 1989; 108: 2163–68.
126 Storey, BT. Sperm capacitation and the acrosome reaction. In: Proceedings of the American Society of Andrology, 16th Annual Meeting, Postgraduate course, Montreal, Canada, 04 1990; 3350.
127 Morales, P, Cross, NL, Overstreet, JW, Hanson, FW. Acrosome intact and acrosome-reacted human sperm initiate binding to the zona pellucida. Dev Biol 1989; 133: 385–92.
128 Franken, DR, Oehninger, S, Burkman, LJ et al. The hemizona assay: a predictor of human sperm fertilizing potential in IVF treatment. J In Vitro Fertil Embryo Transf 1989; 6: 4449.
129 Oehninger, S, Coddington, CC, Scott, R et al. Hemizona assay: assessment of sperm dysfunction and prediction of IVF outcome. Fertil Steril 1989; 51: 665–70.
130 van Uem, JFHM, Acosta, A, Swanson, JR et al. Male factor evaluation in in vitro fertilization: Norfolk experience. Fertil Steril 1985; 44: 375–83.
131 Veeck, LL. Oocyte assessment and biological performance. Ann NY Acad Sci 1988; 541: 259–74.
132 Yoshimatsu, N, Yanagimachi, R, Lopata, A. Zona pellucidae of salt-stored hamster and human eggs: their penetrability by homologous and heterologous spermatozoa. Gamete Res 1988; 21: 115–26.
133 Kruger, TF, Franken, DR, Swart, Y, Oehninger, S. The ability of the hemizona assay to predict sperm fertilizing potential in in vitro in different IVF/GIFT cycles. Proceedings of the 47th Annual Meeting of the American Fertility Society, Orlando, Florida, 10 1991: abstract no. 158.
134 Franken, DR, Kruger, TF, Menkveld, R, Oehninger, S, Coddington, CC, Hodgen, GD. Hemizona assay and teratozoospermia: increasing sperm insemination concentrations to enhance zona pellucida binding. Fertil Steril 1990; 54: 497503.
135 Oehninger, S, Acosta, R, Morshedi, M, Philput, C, Swanson, RJ, Acosta, AA. Relationship between morphology and motion characteristics of human spermatozoa in semen and in the swim-up sperm fractions. J Androl 1990; 11: 446–52.
136 Morales, P, Katz, DF, Overstreet, JW, Samuels, SJ, Chang, RD. The relationship between the motility and morphology of spermatozoa in human semen. J Androl 1988; 9: 241–47.
137 Liu, DY, Baker, HW. The proportion of human sperm with poor morphology but normal intact acrosomes detected with pisum sativum agglutinin correlates with fertilization in vitro. Fertil Steril 1988; 50: 283–93.
138 Courtot, AM, Escalier, D, Jouannet, P, David, G. Impaired ability of human spermatozoa to penetrate zona-free hamster oocytes: is a post-acrosomal sheath anomaly involved? Gamete Res 1987; 17: 145–50.
139 Hammit, DG, Walker, DL, Syrop, CH, Miller, TM, Bennett, MR. Treatment of severe male factor infertility with high concentrations of motile sperm by microinsemination in embryo cryopreservation straws. J In Vitro Fertil Embryo Transf 1991; 8: 101–10.
140 Alexander, NJ. Natural and induced immunological fertility. Curr Opin Immunol 1989; 1: 1125–30.
141 Speroff, L, Friedman, A, Wolf, DPL. Who benefits from intrauterine insemination with washed sperm? Contemp Obstet Gynecol 1990; 35: 6577.
142 Bronson, RA, Cooper, GW, Rosenfeld, DL. Sperm specific isoantibodies and autoantibodies inhibit the binding of human sperm to the human zona pellucida. Fertil Steril 1982; 38: 724–29.
143 Liu, DY, Clarke, GN, Gordon Baker, HW. Inhibition of human sperm-zona pellucida and sperm-oolemma binding by antisperm antibodies. Fertil Steril 1991; 55: 440–42.
144 Tsukui, S, Noda, Y, Fukuda, A, Matsumoto, H, Tatsumi, K, Mori, T. Blocking effect of sperm immobilizing antibodies on sperm penetration of human zonae pellucidae. J In Vitro Fertil Embryo Transf 1988; 5: 123–28.
145 Franken, DR, Windt, ML, Oosthuizen, T et al. Assisted reproductive technologies may obviate apparent immunologic infertility. Andrologia 1991; 23: 291–95.
146 Elder, KT, Wick, KL, Edwards, RG. Seminal plasma antisperm antibodies and IVF: the effect of semen sample collection into 50% serum. Hum Reprod 1990; 5: 179–84.
147 van der Merwe, JP, Kruger, TF, Windt, ML, Hulme, VA, Menkveld, R. Treatment of male sperm autoimmunity by using the gamete intrafallopian transfer procedure with washed spermatozoa. Fertil Steril 1990; 53: 682–87.
148 Hodgen, GD. The hemizona assay: potential utility in predicting contraceptive efficacy and reversibility. CONRAD Communique 1989; 57.
149 Oehninger, S, Alexander, NJ, Franken, DR et al. Antifertilization assays. In: Alexander, N, Griffin, D, Spieler, J, Waites, G eds. Gamete interaction: prospects for immunocontraception. New York: Wiley Liss, 1991:473–88.
150 Rogers, BJ, Wamil, B, Herbert, CM, Pavlou, SM. Use of the SPA and HZA to monitor fertilizing potential of sperm from men receiving a GnRH antagonist. Presented at the 46th Annual Meeting American Fertility Society, Washington DC, 10 15–18, 1990: Abstract no. 076.
151 Oehninger, S, Acosta, A, Hodgen, GD. Antagonistic and agonistic properties of saccharide moieties in the hemizona assay. Fertil Steril 1990; 53: 143–49.
152 Oehninger, S, Clark, GF, Acosta, A, Hodgen, GD. Nature of the inhibitory effect of complex saccharide moieties on the tight binding of human spermatozoa to the human zona pellucida. Fertil Steril 1991; 55: 165–69.
153 Mahoney, M, Oehninger, S, Clark, GF, Acosta, A, Hodgen, GD. Inhibition of zona pellucida-induction of the acrosome relation in human spermatozoa by the complex polysaccharide, fucoidin. Contraception 1991;44:657–65.
154 Cohen, J, Malter, H, Fehilly, C et al. Implantation of embryos after partial opening of oocyte zona pellucida to facilitate sperm penetration [letter]. Lancet 1988; ii: 162.
155 Laws-King, A, Trounson, A, Sathananthan, H, Kola, I. Fertilization of human oocytes by microinjection of a single spermatozoon under the zona pellucida. Fertil Steril 1987; 48: 637–42.
156 Ng, SC, Bongso, A, Ratnam, S et al. Pregnancy after transfer of sperm under zona. Lancet 1988; ii: 790.
157 Lanzendorf, SE, Maloney, MK, Veeck, LL, Slusser, J, Hodgen, GD, Rosenwaks, Z. A preclinical evaluation of pronuclear formation by microinjection of human spermatozoa into human oocytes. Fertil Steril 1988; 49: 835–42.
158 Mortimer, D. Sperm preparation techniques and iatrogenic failures of in vitro fertilization. Hum Reprod 1991; 6: 173–76.
159 Aitken, RJ, Clarkson, JS. Cellular basis of defective sperm function and its association with the genesis of reactive oxygen species by human spermatozoa. J Reprod Fertil 1987; 81: 459–69.
160 Aitken, RJ, Clarkson, JS. Significance of reactive oxygen species and antioxidants in defining the efficacy of sperm preparation techniques. J Androl 1988; 9: 367–76.
161 Aitken, RJ, Clarkson, JS, Hargreave, TB, Irvine, DS, Wu, FC. Analysis of the relationship between defective sperm function and the generation of reactive oxygen species in cases of oligozoospermia. J Androl 1989; 10: 214–20.
162 van der Ven, HH, Hoebbel, K, Al-Liasani, S, Dieddrich, K, Krebs, D. Fertilization of human oocytes in capillary tubes with very small numbers of spermatozoa. Hum Reprod 1989; 4: 7276.
163 Acosta, A, Oehninger, S, Ertunc, H, Philput, C. Possible role of pure human follicle-stimulating hormone in the treatment of severe male-factor infertility by assisted reproduction. Fertil Steril 1991; 55: 1150–56.
164 Menkveld, R, Franken, DR, Kruger, TF, Oehninger, S, Hodgen, GD. Sperm selection capacity of the human zona pellucida. Mol Reprod Dev 1991; 30: 346–52.

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Diagnostic significance of sperm-zona pellucida interaction

  • Sergio Oehninger (a1)

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