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Radiocarbon Dating of Individual Amino Acids from Archaeological Bone Collagen

Published online by Cambridge University Press:  18 July 2016

James S O McCullagh ,
Anat Marom  and
Robert E M Hedges
James S O McCullagh*
Affiliation:
Chemistry Research Laboratory, Mansfield Road, Oxford OX13TA, United Kingdom
Anat Marom
Affiliation:
Research Laboratory for Archaeology and the History of Art (RLAHA), Dyson Perrins Building, South Parks Road, Oxford OX1 3QY, United Kingdom
Robert E M Hedges
Affiliation:
Research Laboratory for Archaeology and the History of Art (RLAHA), Dyson Perrins Building, South Parks Road, Oxford OX1 3QY, United Kingdom
*
Corresponding author. Email: james.mccullagh@chem.ox.ac.uk
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Abstract

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Since the development of accelerator mass spectrometry (AMS) for radiocarbon dating in the late 1970s, its ability to date small samples of bone has been of huge importance in archaeology and Quaternary paleoecology. The conventional approach to sample preparation has been to extract and gelatinize protein, which is then combusted and graphitized for analysis. However, this “bulk protein” can contain a heterogeneous mixture of non-collagenous molecules, including humic acids and other soil components that may be of a different age than the bone and therefore affect the accuracy of its 14C date. Sample pretreatment methods have been an important area of development in recent years but still show inadequacies for the dating of severely contaminated bone. The idea of isolating and dating individual compounds such as single amino acids, to improve dating accuracy, has been discussed in the literature since the 1960s. Hydroxyproline, for example, makes up over 10% of bone collagen but is extremely rare in most other animal proteins, increasing the chances of its presence being endogenous to the individual being dated. Its successful isolation has therefore been considered a potential “gold standard” for dating archaeological bone; however, extracting and suitably purifying single amino acids from bone has proved a challenging task.

Type
Bone Dating and Paleodiet Studies
Information
Radiocarbon , Volume 52 , Issue 2: 20th Int. Radiocarbon Conference Proceedings , 2010 , pp. 620 - 634
Copyright
Copyright © 2010 by the Arizona Board of Regents on behalf of the University of Arizona 

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