Skip to main content Accessibility help

Realizing the potential of electron cryo-microscopy

  • Richard Henderson (a1)


1. Introduction 3

2. Background 5

3. 2D crystals 7

4. 1D crystals (helical arrays) 8

5. Icosahedral single particles 8

6. Single particles with lower symmetry 9

7. Cellular and subcellular electron tomography 10

8. Conclusion and future prospects 10

9. References 11

Structural analysis by electron microscopy of biological macromolecules or macromolecular assemblies embedded in rapidly frozen, vitreous ice has made great advances during the last few years. Electron cryo-microscopy, or cryo-EM, can now be used to analyse the structures of molecules arranged in the form of two-dimensional crystals, helical arrays or as single particles with or without symmetry. Although it has been possible, using crystalline or helical specimens, to reach a resolution adequate to build atomic models (4 Å), there is every hope this will soon also be possible with single particles. Small and large single particles present different obstacles to progress.


Corresponding author

Tel.: +44-1223-402215; Fax: +44-1223-249565; E-mail:

Related content

Powered by UNSILO

Realizing the potential of electron cryo-microscopy

  • Richard Henderson (a1)


Altmetric attention score

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed.