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Monoclonal antibodies in clinical chemistry

  • W. M. Hunter (a1)

Synopsis

Immunoassays have markedly enlarged the armamentarium of the clinical chemist during the last twenty years. Radioimmunoassays and their variants, in particular, now provide evidence which is central to diagnosis and patient management in endocrinology and certain other disorders. Currently used methods employ labelled antigens in the radioimmunoassay (RIA) method first described for insulin by Yalow and Berson. The advent of monoclonal antibodies seems to remove the one major barrier to the use of methods based upon labelled antibodies–the immunoradiometric (IRMA) method as proposed by Miles and Hales. These procedures offer advantages of speed, sensitivity, working range, specificity and robustness which strongly suggest that they will replace RIA for the microdetermination of specific proteins. Nevertheless, their specificity is based upon the recognition of a single epitope on the antigen and thorough testing on varied patient material will be required before they can safely be adopted for routine use.

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van Heyningen, V., Barron, L., Brock, D., Lawrie, S. and Crichton, D. 1982. Monoclonal antibodies to human alpha-foetoprotein: I. Behaviour in radioimmunoassay. J. Immun. Metli. (in press).
Hunter, W. M. 1976. Growth hormone. In Hormone Assays and their Clinical Application, 4th edn, ed. Loraine, J. A. and Bell, E. Trevor, pp. 221284. Edinburgh: Churchill Livingstone.
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Hunter, W. M., Bennie, J. G., Brock, D. J. H. and van Heyningen, V. 1982. Monoclonal antibodies for use in an immunoradiometric assay for x-fetoprotein. J. Immun. Meth. (in press).
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Yalow, R S. and Berson, S. A. 1960. Immunoassay of endogenous plasma insulin in man. J. Clin. Invest. 39, 11571175.
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Monoclonal antibodies in clinical chemistry

  • W. M. Hunter (a1)

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