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Free radical processes induced by desiccation in germinating maize. The relationship with respiration and loss of desiccation tolerance

  • O. Leprince (a1), G. A. F. Hendry (a2) and N. M. Atherton (a3)

Synopsis

Using germination of maize as a model, desiccation-induced free radical processes were studied with the object of understanding desiccation tolerance. Several significant elements of damage were observed in desiccated material associated with development of desiccation intolerance: increased lipid peroxidation, phospholipid de-esterification, build-up of a stable free radical, supression or repression of respiratory enzymes from complex I, II and IV. An EPR (electron paramagnetic resonance) response was also detected in isolated mitochondria following in vitro desiccation. The loss of desiccation tolerance appeared to be dependent on oxygen concentration. Two highly significant correlations were independently found between respiration rates and production of a stable free radical detected by EPR. These data suggest that respiration is an important factor in the loss of desiccation tolerance. We present a model suggesting that activated oxygen formation during desiccation originates in the disruption of the mitochondrial electron transport chain with increasing leakage to oxygen so generating irreversible and lethal peroxidative damage, leading to the development of desiccation intolerance.

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Blackman, S. A., Obendorf, R. L. & Leopold, A. C. 1992. Maturation proteins and sugars in desiccation tolerance of developing soybean seeds. Plant Physiology 100, 225–30.
Cadenas, E. 1989. Biochemistry of oxygen toxicity. Annual Review of Biochemistry 58, 79110.
Halliwell, B. 1987. Oxidative damage, lipid peroxidation and antioxidant protection in chloroplasts. Chemistry and Physics of Lipids 44, 327–40.
Hendry, G. A. F., Finch-Savage, W. E., Thorpe, P. C., Atherton, N. M., Buckland, S. H., Nilsson, K. A. & Seel, W. E. 1992. Free radical processes and loss of viability during desiccation in the recalcitrant species Quercus robur L. New Phytologist 122, 273–99.
Hendry, G. A. F. & Grime, J. P. 1993. Methods in comparative plant ecology. London: Chapman and Hall.
Leprince, O., Deltour, R., Thorpe, P. C., Atherton, N. M. & Hendry, G. A. F. 1990. The role of free radicals and radical processing systems in loss of desiccation tolerance in germinating maize (Zea mays L.) New Phytologist 116, 573–80.
Leprince, O., Van der Werf, A., Deltour, R. & Lambers, H. 1992. Respiratory pathways in germinating maize radicles correlated with desiccation tolerance and soluble sugars. Physiologia Plantarum 85, 581–8.
Leprince, O., Hendry, G. A. F. & McKersie, B. D. 1993. Mechanisms of desiccation tolerance in developing seeds. Seed Science Research 3, 231–46.
Leprince, O., Atherton, N. M., Deltour, R. & Hendry, G. A. F. 1994. The involvement of respiration in free radical processes during loss of desiccation tolerance in germinating Zea mays L. An electron paramagnetic resonance study. Plant Physiology 104, 1333–9.
McKersie, B. D. 1991. The role of oxygen free radicals in mediating freezing and desiccation stress in plants. In Pell, E. J. & Steffen, K. (Eds) Active oxygen/oxidative stress and plant metabolism, pp. 107–18. Rockville, MD: American Society of Plant Physiology.
McKersie, B. D., Hoekstra, F. A. & Krieg, L. C. 1990. Differences in the susceptibility of plant membrane lipids to peroxidation. Biochimica Biophysica Ada 1030, 119–26.
Puntarulo, S., Galleano, M., Sanchez, R. A. & Boveris, A. 1991. Superoxide anion and hydrogen peroxide metabolism in soybean embryonic axes during germination. Biochimica Biophysica Acta 1074, 277–83.
Seel, W. E., Hendry, G. A. F., Atherton, N. M. & Lee, J. 1991. Radical formation and accumulation in vivo, in desiccation tolerant and intolerant mosses. Free Radical Research Communications 15, 133–41.
Senaratna, T. & McKersie, B. D. 1986. Loss of desiccation during seed germination. A free radical mechanism of injury. In Leopold, A. C. (Ed.) Membranes metabolism and dry organisms. Ithaca, NY: Cornell University Press.
Senaratna, T., McKersie, B. D. & Borochov, A. 1987. Desiccation and free radical mediated changes in plant membranes. Journal of Experimental Botany 38, 2005–14.
Turrens, J. F., Beconi, M., Barilla, J., Chavez, U. B. & McCord, J. M. 1991. Mitochondrial generation of oxygen radicals during reoxygenation of ischemic tissues. Free Radical Research Communications 12/13, 681–9.

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Free radical processes induced by desiccation in germinating maize. The relationship with respiration and loss of desiccation tolerance

  • O. Leprince (a1), G. A. F. Hendry (a2) and N. M. Atherton (a3)

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