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Primary structure of arabinoxylans of ispaghula husk and wheat bran.

Published online by Cambridge University Press:  05 March 2007

Sandra Edwards ,
Martin F. Chaplin ,
Anne D. Blackwood  and
Peter W. Dettmar
Sandra Edwards
Affiliation:
South Bank University, 103 Borough Road, London, SE1 OAA, UK
Martin F. Chaplin*
Affiliation:
South Bank University, 103 Borough Road, London, SE1 OAA, UK
Anne D. Blackwood
Affiliation:
South Bank University, 103 Borough Road, London, SE1 OAA, UK
Peter W. Dettmar
Affiliation:
ReckittBenckiser Healthcare (UK) Ltd, Dansom Lane, Kingston upon Hull, HU8 7DS, UK
*
*Corresponding author: Dr Martin Chaplin, fax +44 20 7815 7999, martin.chaplin@sbu.ac.uk
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Abstract

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he primary structures of ispaghula husk and wheat bran were investigated in order to determine how and why these fibres are among the most beneficial dietary fibres. To this end, the polysaccharide preparations have been subjected to enzymic hydrolysis and methylation analysis. The results have shown ispaghula husk and wheat bran to be very-highly-branched arabinoxylans consisting of linear Β-D-→l(4)-linked xylopyranose →Xylp) backbones to which a-L-arabinofuranose →Araf) units are attached as side residues via α-→1(3) and a-→l(2) linkages. Other substituents identified as present in wheat bran include Β-D-glucuronic acid attached via the C→O)-2 position, and arabinose oligomers, consisting of two or more arabinofuranosyl residues linked via 1–2, 1–3, and 1–4 linkages. Ispaghula-husk arabinoxylan is more complex having additional side residues which include a-D-glucuronopyranose →GalAp)-→l→2(-linked-α-L-rhamnopyranose-(1→4)-β-D-Xylp, α-D-GalA/>-→l→3(-linked-a-L-Araf-→1)4(-p-D-Xylp, and <x-L-Araf-→1→3(-linked-p-D-Xylp-→1→4(-β-D-Xylp. The beneficial effects of increased faecal bulk and water-holding capacity are undoubtedly related to the structures of the arabinoxylans, with differences in their efficacy to treat various functional bowel disorders due to their specific structural features

Keywords

Dietary fibreArabinoxylanIspaghula huskWheat bran
Type
Session: Physiological aspects of fibre
Information
Proceedings of the Nutrition Society , Volume 62 , Issue 1 , February 2003 , pp. 217 - 222
Copyright
Copyright © The Nutrition Society 2003

References

Bengtsson, S & Aman, P (1990) Isolation and chemical characterisation of water soluble arabinoxylans in rye grain. Carbohydrate Polymers 12, 267–277.CrossRefGoogle Scholar
Bergmans, MEF, Beldman, G, Gruppen, H & Voragen, AGJ (1996) Optimisation of the selective extraction of (glucurono) arabinoxylans from wheat bran: Use of barium and calcium hydroxide solution at elevated temperatures. Journal of Cereal Science 23, 233–245.CrossRefGoogle Scholar
Dubois, M, Gilles, KA, Hamilton, JK, Rebers, PA & Smith, F (1956) Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28, 350–356.CrossRefGoogle Scholar
Fincher, GB & Stone, BA (1986) Cell walls and their components in cereal grain technology. In Advances in Cereal Science and Technology. pp.207 – 295, [Pomeranz, Y, editors] St Paul, MN: American Association of Cereal Chemists Inc.Google Scholar
Gruppen, H, Kormelink, FJM & Voragen, AGJ (1993) Water un-extractable cellwall material from wheat flour. 3. A structural model for arabinoxylans. Journal of Cereal Science 18, 111–128.CrossRefGoogle Scholar
Hakomori, S-I (1964) A rapid permethylation of glycolipids and polysaccharides catalyses by methylsulfinyl carbanion in dimethyl sulfoxide. Journal of Biochemistry (Tokyo) 55, 205–208.Google Scholar
Hoffmann, RA, Kamerling, JP & Vliegenthart, JFG (1992) Structural features of a water-soluble arabinoxylan from the endosperm of wheat. Carbohydrate Research 226, 303–311.CrossRefGoogle Scholar
Izydorczyk, MS & Biliaderis, CG (1995) Cereal arabinoxylans: advances in structure and physicochemical properties. Carbohydrate Polymers 28, 33–48.CrossRefGoogle Scholar
Kennedy, JF, Sandhu, JS & Southgate, DAT (1979) Structural data for the carbohydrate of ispaghula husk ex Plantago ovata Forsk. Carbohydrate Research 75, 265–274.CrossRefGoogle Scholar
Lowry, OH, Rosebrough, NJ, Farr, AL & Randall, RJ (1951) Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–275.CrossRefGoogle ScholarPubMed
Shiiba, K, Yamada, H, Kara, H, Okada, K & Nagao, S (1993) Purification and characterisation of two arabinoxylans from wheat bran. Cereal Chemistry 70, 209–214.Google Scholar
Somogyi, MJ (1952) Determination of blood sugar. Journal of Biological Chemistry 195, 19–23.CrossRefGoogle Scholar
Stevens, BJH & Selvendran, RR (1988) Changes in composition and structure of wheat bran resulting from the action of human fecal bacteria in vitro. Carbohydrate Research 183, 311–319.CrossRefGoogle ScholarPubMed
Sweet, DP, Shapiro, RH & Albersheim, P (1975) Quantitative analysis by various GLC response factor theories for partially methylated and partially ethylated alditol acetates. Carbohydrate Research 40, 217–255.CrossRefGoogle Scholar
Taylor, RA & Conrad, HE (1972) Stoichiometric depolymerisation of polyuronides and glucosaminoglycuronans to monosaccharides following reduction of their carbodiimide activated carboxyl group. Biochemistry 11, 1383–1388.CrossRefGoogle Scholar