Skip to main content Accessibility help
×
Home

Contents:

Information:

  • Access

Actions:

      • Send article to Kindle

        To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

        Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

        Find out more about the Kindle Personal Document Service.

        MicroRNA expression in the macroscopically-normal epithelium of people at differential risk of colorectal cancer
        Available formats
        ×

        Send article to Dropbox

        To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

        MicroRNA expression in the macroscopically-normal epithelium of people at differential risk of colorectal cancer
        Available formats
        ×

        Send article to Google Drive

        To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

        MicroRNA expression in the macroscopically-normal epithelium of people at differential risk of colorectal cancer
        Available formats
        ×
Export citation

Colorectal cancer (CRC) is the third most common cancer in the UK. The majority of CRC cases are sporadic( 1 ), and the presence of adenomatous polyps or ulcerative colitis (UC) increases CRC risk. In preparation for a study of the effects of a dietary intervention, the present study investigated the expression of 5 microRNAs (miRNAs) reported to be altered in CRC, involved in the WNT pathway and/or implicated in the inflammatory process in people at higher-risk of CRC.

Colorectal mucosal biopsies were collected from normal participants (n=10), participants with quiescent UC (n=9) and participants with prior history of adenomatous polyps (n=9). RNA was extracted from formalin-fixed paraffin-embedded (FFPE) biopsies and reverse transcribed to synthesize cDNA used to quantify the expression of a panel of miRNAs: miR-101, miR-335, miR-122a, miR-135a and miR-145, in duplicate by quantitative PCR. Statistical analysis was performed using the ANOVA General Linear Model.

With relatively small participant numbers, no statistically significant differences were observed for the expression of the selected miRNAs between the 3 groups. However, miR-335 expression, frequently upregulated in CRC, appeared to be increased in polyp patients (Fig. 1A). Participants with UC, an inflammatory disease, appeared to have slightly reduced miR-335 expression, consistent with previous evidence that miR-335 is down-regulated by inflammatory cytokines( 2 ). In addition, miR-101 and miR-145, both down-regulated in CRC( 3 , 4 ), appeared to be reduced in higher-risk participants (Fig. 1B and C).

Fig. 1. Mean expression of miR-335 (A), miR-101 (B) and miR-145 (C), expressed as 2−ΔCt×1000 relative to the SNORD68 control.

This study has quantified miRNA expression in FFPE tissue biopsies of normal colorectal mucosa and found evidence of potential differences in those at higher CRC risk. To investigate underlying mechanisms, the methodology developed in this study will be used to measure miRNA expression in healthy participants supplemented with non-digestible carbohydrates, thought to be protective against CRC.

This study was funded by the BBSRC (BB/H005013/1). Ethical approval for this project, as part of a larger application, was granted on 10th December 2009 (REC No. 09/H0907/77).

1. Fearnhead, NS, Wilding, JL & Bodmer, WF (2002) Br Med Bull 64, 2743.
2. Tome, M, Lopez-Romero, P, Albo, C et al. (2011) Cell Death Differ 18, 985–95.
3. Strillacci, A, Griffoni, C, Sansone, P et al. (2009) Exp Cell Res 315, 1439–47.
4. Zhang, J, Guo, H, Zhang, H et al. (2011) Cancer 117, 8695.