Skip to main content Accessibility help
×
Home
Hostname: page-component-99c86f546-z5d2w Total loading time: 0.943 Render date: 2021-12-09T10:56:45.162Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": true, "newCiteModal": false, "newCitedByModal": true, "newEcommerce": true, "newUsageEvents": true }

Influence of vitamin B12 status and different dietary levels of folic acid on several immune parameters in aged rats

Published online by Cambridge University Press:  04 June 2010

T. Partearroyo
Affiliation:
Department of Nutrition, Food Sciences and Food Technology, Faculty of Pharmacy, San Pablo CEU University, 28668 Madrid, Spain
N. Úbeda
Affiliation:
Department of Nutrition, Food Sciences and Food Technology, Faculty of Pharmacy, San Pablo CEU University, 28668 Madrid, Spain
M. Achón
Affiliation:
Department of Nutrition, Food Sciences and Food Technology, Faculty of Pharmacy, San Pablo CEU University, 28668 Madrid, Spain
A. Montero
Affiliation:
Department of Nutrition, Food Sciences and Food Technology, Faculty of Pharmacy, San Pablo CEU University, 28668 Madrid, Spain
M. De La Fuente
Affiliation:
Department of Physiology, Faculty of Biology, Complutense University of Madrid, 28040 Madrid, Spain
N. M. De Castro
Affiliation:
Department of Physiology, Faculty of Biology, Complutense University of Madrid, 28040 Madrid, Spain
E. Alonso-Aperte
Affiliation:
Department of Nutrition, Food Sciences and Food Technology, Faculty of Pharmacy, San Pablo CEU University, 28668 Madrid, Spain
G. Varela-Moreiras
Affiliation:
Department of Nutrition, Food Sciences and Food Technology, Faculty of Pharmacy, San Pablo CEU University, 28668 Madrid, Spain
Rights & Permissions[Opens in a new window]

Abstract

Type
Abstract
Copyright
Copyright © The Authors 2009

Intake of folic acid (FA) in very high amounts leads to conditions similar to those of metabolic deficiency in folate(Reference Troen1). In a similar way, vitamin B12 status influences the physiological response to different FA doses in the organism(Reference Morris2). Several studies relate both the shortage of folate as well as its excess with a change in the immune response(Reference Field3). Similarly, it has been found that vitamin B12 may play an important role in cellular immunity. Therefore, an imbalance between the status of vitamin B12 and FA could exacerbate the immune response(Reference Smith4).

The aim of this study was to evaluate the global effect of different levels of dietary FA and vitamin B12 on several parameters of the immune response in aged rats.

Twenty-month male Sprague–Dawley rats (n=40) divided into four groups were fed during 30 days with differently supplemented diets:

  • CONB12/CONFA (50 μg vitamin B12; 2 mg FA)

  • DEFB12/DEFFA (0 μg vitamin B12; 0 mg FA)

  • DEFB12/CONFA (0 μg vitamin B12; 2 mg FA)

  • DEFB12/SUPFA (0 μg vitamin B12; 8 mg FA)

We evaluated the natural killer (NK) cytotoxicity in spleen, thymus and axillary nodes and lymphocyte subsets in peripheral blood.

There was a significant decrease in the NK cytotoxicity in the spleen, in the DEFB12/CONFA group (P<0.05), but neither in the thymus nor the axillary nodes. Furthermore, we observed a significant decrease in the CD45 lymphocyte subsets in the groups DEFB12/CONFA and DEFB12/SUPFA (P<0.05) but not in lymphocyte subsets CD4, CD8, CD3 and CD161.

An imbalance between B12 and FA concentrations during ageing alters some immunological parameters such as the NK citotoxicity and CD45 lymphocyte subsets. These effects have not been observed in a control (CONB12/CONFA) situation or even during deficiency of both vitamins (DEFB12/DEFFA). Therefore, the balance between the intake of folate and vitamin B12 could be as important as their absolute concentrations in the diet.

References

1.Troen, AM et al. (2006) J Nutr 136, 189194.Google Scholar
2.Morris, MS et al. (2007) Am J Clin Nutr 85, 193200.Google Scholar
3.Field, CJ et al. (2005) J Nutr Biochem 17, 3744.CrossRefGoogle Scholar
4.Smith, D (2007) Am J Clin Nutr 85, 35.Google Scholar
Figure 0

You have Access

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Influence of vitamin B12 status and different dietary levels of folic acid on several immune parameters in aged rats
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Influence of vitamin B12 status and different dietary levels of folic acid on several immune parameters in aged rats
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Influence of vitamin B12 status and different dietary levels of folic acid on several immune parameters in aged rats
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *