It has been shown that exercise induces considerable changes in the body, particularly in the mucosal immune system(1); the current lifestyles have promoted an increase in the consumption of high carbohydrate diets (↑CHO); these two factors result in particular effects on Peyer's patches (PP)(2). The objective of this study was to evaluate the effect of moderate exercise and ↑CHO on PP's T-cells (TLc) in Balb/c young mice. Thirty-two 21-d-old male mice were divided into four groups, two groups were taught to swim for 30 min 5 d per week during 9 weeks; one of which was fed a commercial control diet (CONTROL/E) and the other was fed a high carbohydrate diet (↑CHO/E) (DIO Rodent Purified Diet, Cat. 58Y2; energy: 4.65 kcal/g (19.4556 kJ/g), the other two groups were fed the same diets but were not made to swim (CONTROL and ↑CHO). Small intestines were dissected, PP were cut, macerated, filtered and centrifuged to obtain the lymphocyte pellet; cells were stained with anti-CD3+, CD4+ and CD8+ antibodies and analysed by flow cytometry (Facs Diva, BD®). The experimental diet increased the weight of mice who exercised (23.5, sd 1.3) compared with the non-exercise group (19.4, sd 1.8) and the control group (20.2, sd 1.4), P⩽0.005. CD3+ TLc were decreased in the ↑CHO/E group (31.1, sd 1.0) compared with the CONTROL/E group (38.8, sd 2.5), T 8.103, P⩽0.001; CD3+/CD4+ (23.2, sd 0.2), CD3+/CD8+ (3.5±0.5) also decreased compared with CONTROL/E group (33.5, sd 1.97 and 4.9, sd 0.5; t 14.8 and t 6.014 respectively, P⩽0.001). The combination of exercise and ↑CHO decreased significantly PP TLc, we can conclude that the intake of a high carbohydrate diet together with exercise attenuates lymphocytes transit, as well as increasing the weight of the mice (Fig. 1).
This project was financed by Universidad Autónoma del Estado de México.