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The viability of ovine embryos recovered transcervically and transferred by laparoscope

Published online by Cambridge University Press:  20 November 2017

M J A Mylne ,
W S Dingwall ,
M E King ,
W A C McKelvey  and
J J Robinson
M J A Mylne
Affiliation:
SAC, West Mains Road, Edinburgh EH9 3JG, UK
W S Dingwall
Affiliation:
SAC, West Mains Road, Edinburgh EH9 3JG, UK
M E King
Affiliation:
SAC, Craibstone, Bucksburn, Aberdeen AB21 9YA, UK
W A C McKelvey
Affiliation:
SAC, West Mains Road, Edinburgh EH9 3JG, UK
J J Robinson
Affiliation:
SAC, Craibstone, Bucksburn, Aberdeen AB21 9YA, UK
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Extract

In previous studies (Mylne et al, 1992; McKelvey et al, 1997)) we have shown that the recovery of sheep embryos via the cervix can be facilitated by combining topical application of prostaglandin E2 (PGE2) to the external os cervix with systemic oestradiol treatment (E2) to dilate the cervix. The combined PGE2 and E2 treatment did not inhibit early embryo development (McEvoy et al, 1996) but conceptus viability was not determined. The objective of the present study was to test the viability of embryos recovered transcervically following dilation of the cervix with exogenous hormones and provide quantative data on their subsequent ability to establish normal pregnancies.

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Programme
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Proceedings of the British Society of Animal Science , Volume 1999 , 1999 , pp. 16
Copyright
Copyright © The British Society of Animal Science 1999

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References

Mylne, MJA, McKelvey, WAC, Fernie, K and Matthews, KP, 1992. A report on the use of transcervical technique for embryo recovery in the sheep. Veterinary Record 130, 450–451 CrossRefGoogle Scholar
McKelvey, WAC, McEvoy, TG, Dingwall, WS, Robinson, JJ, Lindsay, E, King, ME, FitzSimons, J and Mylne, MJA, 1997. The use of exogenous hormones to facilitate transcervical embryo recovery in sheep and their effect on embryo development. Theriogenology 47, 369.Google Scholar
McEvoy, TG, McKelvey, WAC, Robinson, JJ, Dingwall, WS, Lindsay, E, King, ME and FitzSimons, J, 1996. Treatment of ewes to facilitate transcervical embryo collection does not compromise embryo development. Journal of Reproduction and Fertility 17, 6.Google Scholar