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The use of 17 RAPD primers in some of Iranian sheep breeds

Published online by Cambridge University Press:  20 November 2017

M. Tahmoorespur ,
M. R. Nassiry  and
A. Mohammady
M. Tahmoorespur*
Affiliation:
Dept of Animal Science, College of Agric, Ferdowsi Univ.P.O.Box: 91775-1163, Mashhad, Iran
M. R. Nassiry
Affiliation:
Dept of Animal Science, College of Agric, Ferdowsi Univ.P.O.Box: 91775-1163, Mashhad, Iran
A. Mohammady
Affiliation:
Dept of Animal Science, College of Agric, Ferdowsi Univ.P.O.Box: 91775-1163, Mashhad, Iran
*
E-mail: m_tahmoorespur@yahoo.com
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Extract

The randomly amplified polymorphic DNA technique was developed by Williams et al,(1990) and Welsh and McClelland et al.(1990). The technique is based on polymerase chain reaction using primers homologous to random target sites in the genome. The main advantages of RAPD assays are that it is simple, less labour intensive, comparatively less expensive and safe than other methods. The RAPD hase been used for various applications including species identification, establishing genetic relationships, estimating genetic diversity and genome mapping in various livestock species including sheep. These studies reflected the effectiveness of rapd as potential genetic marker. The objective of the current study was to investigate RAPD marker development to distinguish genetic differences and similarity between and within some Iranian sheep breeds.

Type
Genetics
Information
Proceedings of the British Society of Animal Science , Volume 2003 , 2003 , pp. 144
Copyright
Copyright © The British Society of Animal Science 2003

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References

Kantanen, J., Vilkki, J., Elo, K. and Maki-Tanila, A. 1995. Random amplified polymorphic DNA in cattle and sheep: application for detecting genetic variation. Animal Genetics. 25: 315–320.Google Scholar
Wesh, J. and McClelland, M. 1990. Fingerprinting genome using PCR with arbitrary primers. Nucl. Acid Res. 18: 7213–7218.Google Scholar
Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A. and Tingey, S. V. 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucl. Acid Res. 18: 6531–6535.Google Scholar