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Urinary excretion of allantoin in steers fed on cereal based diet infused with different levels of urea-casein

  • S. M. Martín-Orúe (a1), J. Balcells (a1), F. Vicente (a1) and J. A. Guada (a1)

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Values of ammonia concentration in rumen liquid have been used as an index of nitrogen requirements of rumen microflora even though ammonia requirements seem to depend on the type of diet. Cellulollitic flora mainly use ammonia as N source, while bacteria that ferment non-structural carbohydrates use either ammonia or protein nitrogen. The aim of this experiment was to establish the minimum concentration of ammonia to optimise microbial synthesis in the rumen of steers fed concentrate-based diets.

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AFRC 1993. Energy and Protein Requirements of Ruminants. CAB International, Wallingford, UK.
Balcells, J., Guada, J. A., Peiró, J. M. and Parker, D. S. 1992. Simultaneous determination of allantoin and oxypurines in biological fluids by high-performance liquid chromatography. Journal of Chromatography 575: 153157.
Chaney, A. L. and Marbach, E. P. 1962. Modified reagents for determination of urea and ammonia. Clinical Chemistry 8: 130132.

Urinary excretion of allantoin in steers fed on cereal based diet infused with different levels of urea-casein

  • S. M. Martín-Orúe (a1), J. Balcells (a1), F. Vicente (a1) and J. A. Guada (a1)

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