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Tannin bioassay using semi-automated gas production technique

  • E.F. Nozella (a1), S.L.S. Cabral Filho (a1), I.C.S. Bueno (a1), P.B. Godoy (a1), C. Longo (a1), A.L. Abdalla (a1) and D.M.S.S. Vitti (a1)...


Brazil has arid regions where livestock production is limited by forage source. However, some native herbaceous browses have a dry tolerance and had been used as animal feed. Some of those plants have anti nutritional compounds such as tannins that can interfere on intake and digestibility. Tannins have a high affinity to proteins and could make these molecules unavailable for animal. Compounds as polyethylene glycol (PEG) has been used on tannin studies, because it has more affinity with tannins than proteins. Based on that, it is possible to evaluate the nutritive potential of tanniniferous plants, using PEG in gas based techniques for assessing anti nutritional factors in tanniniferous plants for ruminants. The aim of this work was to investigate the effects of different treatments (oven-, shade- and sun-drying and treatment with urea) on phenolics compounds and on the biological activity of tannins using the in vitro gas method with the addition of polyethylene glycol (PEG).


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Bueno., I.C.S.,; Gobbo., S.P.,; Abdalla., A.L., and Cabral Filho., S.L.S., 2000. Effect of solid phase of rumen liquor on the inoculum used for in vitro gas production technique. Gas production: fermentation kinetics for feed evaluation and to assess microbial activity. 2122. EAAP/BSAS, Penicuik.
Makkar., H.P.S., 2000. Quantification of tannins in tree foliage. Vienna: FAO; IAEA, 2000. cap.3, p.6-8: Measurement of total phenolics and tannins using Folin-Ciocalteu method. (Laboratory manual).
Mauricio., R.M.,; Mould., F.L.,; Dhanoa., M.S.,; Owen, E.; Channa., K.S.,; Theodorou., M.K., 1999. A semi-automated in vitro gas production tecnique for ruminant feedstuff evaluation. Animal Feed Science and Technology, 42: 321333.
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