The antibiotic use to increase the animal production can result in residual concentrations in products as meat, eggs and milk, exceeding the acceptable doses for human consumption. In Brazil nowadays, 14 antibiotics are employed as feed additive, however there are about 121 drugs used in Veterinary Medicine that contain antibiotics in their formulation. Due to amplitude and frequency of the antimicrobial resistance, it can be considered an emergent and significant question for the public health and an alert for the necessity of a global control about the use of those antibiotics. The immunoassays allow the diagnosis of these antibiotics with accessible costs and presenting good results. The aim of this work was to produce polyclonal antibodies for ciprofloxacin detection using ELISA.