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Predicting the in vitro gas production profile of dried grass with strained rumen fluid from the in vitro gas production profile of dried grass with faeces

Published online by Cambridge University Press:  20 November 2017

C. Rymer
Affiliation:
ADAS Nutritional Sciences Research Unit, Alcester Road, Stratford-on-Avon, CV37 9RQ United Kingdom
D.I. Givens
Affiliation:
ADAS Nutritional Sciences Research Unit, Alcester Road, Stratford-on-Avon, CV37 9RQ United Kingdom
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Extract

The in vitro gas production (GP) technique was developed using strained rumen fluid (SRF) as an inoculum. This requires the use of surgically modified animals and it is questionable whether maintaining cannulated animals for this purpose can be justified. Faeces (FAE) have been used as an alternative inoculum, but while total gas volume tends to be the same, the rate of gas production is usually slower. This is probably because of a lower microbial activity in FAE. If the initial activities of the two inoculum sources were controlled, the differences between them may be reduced. The objective of this experiment, therefore, was to compare the GP profiles obtained when different concentrations of SRF and FAE were used, and the initial activity of the inocula equalised.

Type
Poster Presentations
Copyright
Copyright © The British Society of Animal Science 2000

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References

Groot, J.C.J., Cone, J.W., Williams, B.A., Debersaques, F.M.A. and Lantinga, E.A. (1996) Multiphasic analysis of gas production kinetics for in vitro fermentation of ruminant feeds. Animal Feed Science and Technology. 64: 7789.Google Scholar
Nagadi, S., Herrero, M. and Jessop, N.S. (1999) Effect of frequency of ovine ruminal sampling on microbial activity and substrate fermentation. Proceedings of the British Society of Animal Science 1999. p. 154.Google Scholar