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Immunohistochemical localisation of aquaporin water channels in the bovine mammary gland

Published online by Cambridge University Press:  23 November 2017

B. Kendall ,
J.E.J. Maxwell ,
A.J. German ,
D. Marples ,
M.D. Royal  and
A. Mobasheri
B. Kendall
Affiliation:
Faculty of Veterinary Science, University of Liverpool, Leahurst, South Wirral, CH64 7TE, United Kingdom
J.E.J. Maxwell
Affiliation:
Faculty of Veterinary Science, University of Liverpool, Leahurst, South Wirral, CH64 7TE, United Kingdom
A.J. German
Affiliation:
Faculty of Veterinary Science, University of Liverpool, Leahurst, South Wirral, CH64 7TE, United Kingdom
D. Marples
Affiliation:
Institute of Membrane and Systems Biology, University of Leeds, Leeds, LS2 9JT, United Kingdom
M.D. Royal*
Affiliation:
Faculty of Veterinary Science, University of Liverpool, Leahurst, South Wirral, CH64 7TE, United Kingdom
A. Mobasheri
Affiliation:
School of Veterinary Medicine, University of Nottingham, Sutton Bonington Campus, Sutton Bonington, Leicestershire, LE12 5RD, United Kingdom
*
Email:mdroyal@liverpool.ac.uk
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Extract

The aquaporins (AQPs) are a family of small integral membrane proteins 28-35kDa in size, sub classified into 2 groups; those selectively permeated by water (the aquaporins; AQP0, AQP1, AQP2, AQP4, AQP5, AQP6 and AQP8) or by water and small organic osmolytes such as glycerol and urea (the aquaglyceroporins; AQP3, AQP7, AQP9 and AQP10). AQPs are expressed in a variety of epithelial tissues where they are responsible for rapid water movement driven by osmotic gradients. The mammary gland produces and secretes milk which consists of water, lipids, electrolytes, vitamins, sugars and specific milk proteins. However, little is known about AQP expression, distribution and function in mammary tissue or about the process of water transport across the mammary epithelium. The only information available relates to the identification of AQP1 and AQP3 in capillary endothelia and epithelial cells of mouse mammary glands respectively (Matsuzaki et al., 2005). Since water delivery to the mammary gland is critical for milk production, which is of major economic importance to the dairy industry, it is necessary to understand the underlying physiology involved. The aim of this study was to determine the expression and distribution of AQPs within the bovine mammary gland.

Type
Theatre Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2007 , April 2007 , pp. 1
Copyright
Copyright © The British Society of Animal Science 2007

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References

Matsuzaki, T., Machida, N., Tajika, Y, Ablimit, A., Suzuki, T., Aoki, T., Hagiwara, H., Takata, K. (2005). Expression and immunolocalization of water-channel aquaporins in the rat and mouse mammary gland. Histo Cell Biol. 123:501-12.CrossRefGoogle ScholarPubMed
Mobasheri, A. and Marples, D. (2004). Expression of the AQP-1 water channel in normal human tissues: a semiquantitative study using tissue microarray technology. Am J Physiol Cell Physiol. 286: C529-37.CrossRefGoogle ScholarPubMed