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Gas volume and microbial growth relationship using in vitro techniques related to feed quality

  • C. Longo (a1), S. P. Gobbo (a1), I.C.S. Bueno (a1), S.L.S. Cabral Filho (a1) and A.L. Abdalla (a1)...

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The gas measured in an in vitro experiment is an indirect measurement of nutritionally important events. Feeds in the rumen are converted to short-chain fatty acids, to CO2, to CH4 and to microbial mass plus water. The pathways used for microorganisms for carbohydrate degradation will determine the amount of ATP available for microbial cell production and vary with the type of substrate, nature of inoculum and time of observation. The aim of this work was to study the relationship between gas volume and microbial growth regarding to the substrate quality.

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France, J.; Dhanoa, M.S.; Theodorou, M.K.; Lister, S.J.; Davies, S.J. and Isac, D., 1993. A model to interpret gas accumulation profiles with in vitro degradation of ruminant feeds. Journal of Theoretical Biology, 163:99111.
Orskov, E.R.; McDonald, I., 1979. The estimation of protein degradability in the rumen from incubation measurements weighted according to rate of passage. Journal of American Science, v.92, p.449453.
Theodorou, M.K.; Williams, B.A.; Dhanoa, M.S.; McAllan, A.B. and France, J., 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology, 48:18597.
Cabral Filho, S.L.S.; Gobbo, S.P.; Bueno, I.C.S.; Machado, M.C.; Nozella, E.F.; Abdalla, A.L., 2002. Incorporação in vitro de radiofósforo pelos microrganismos ruminais em diferentes proporções do inóculo. Revista Brasileira de Pesquisa e Desenvolvimento, 4 (3): 15381540.
Bueno, I.C.S.; Machado, M.C.; Cabral Filho, S.L.S.; Gobbo, S.P.; Vitti, D.M.S.S.; Abdalla, A.L., 2002. Rumen microbial growth estimation using in vitro radiophosphorous incorporation technique. Revista Brasileira de Pesquisa e Desenvolvimento, 4 (3): 15341537.

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