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Gas production in vitro from purified starches using equine faeces as the source of inocula

Published online by Cambridge University Press:  20 November 2017

B M L McLean ,
J J Hyslop ,
A C Longland ,
D Cuddeford  and
T Hollands
B M L McLean
Affiliation:
Dept. Of Vet. Clinical Studies, University of Edinburgh, Easter Bush, Roslin, Midlothian, EH25 9RG, UK
J J Hyslop
Affiliation:
Dept. Of Vet. Clinical Studies, University of Edinburgh, Easter Bush, Roslin, Midlothian, EH25 9RG, UK
A C Longland
Affiliation:
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, SY23 3EB, UK
D Cuddeford
Affiliation:
Dept. Of Vet. Clinical Studies, University of Edinburgh, Easter Bush, Roslin, Midlothian, EH25 9RG, UK
T Hollands
Affiliation:
Dodson & Horrell Ltd, Ringstead, Kettering, Northamptonshire, NN14 4BX
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Extract

In vitro techniques have been developed to study the fermentation kinetics of a wide range of animal feedstuffs but relatively few studies have been conducted specifically with purified feed constituents. This study uses the pressure transducer technique of Theodorou et al (1994) to record cumulative gas production (GP) when six purified starch sources were incubated in vitro.

Three replicates of six commercially available purified (98%) starch sources were incubated in vitro with an inoculum prepared from freshly voided faeces collected from six ponies fed hay ad libitum. The starches were a purified wheat starch (ABRA), four purified wheat starches that had been chemically modified with sodium tri-metaphosphate (V1, V21, V33 & V65) and a purified pea starch (PEA). GP was measured using the pressure transducer technique throughout a 72 h incubation period. At the end of the incubation period DM loss (DML) in vitro was determined by filtration.

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Programme
Information
Proceedings of the British Society of Animal Science , Volume 1999 , 1999 , pp. 132
Copyright
Copyright © The British Society of Animal Science 1999

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References

France, J., Dhanoa, M.S., Theodorou, M.K., Lister, S.J., Davies, D.R., Isac, D. 1993. A model to interpret gas accumulation profiles associated with in vitro degradation of ruminant feeds. Journal of Theoretical Biology 163: 99– 111.Google Scholar
Theodorou, M.K., Williams, B.A., Dhanoa, M.S., McAllan, A.B., France, J. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science & Technology 48: 185–197.CrossRefGoogle Scholar