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Fibrolytic enzymes increase the hydrolysis and rate of fermentation of pure substrates in vitro

  • D. Colombatto (a1) (a2), F. L. Mould (a1), M. K. Bhat (a2) and E. Owen (a1)

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Treatment of forage with enzyme mixtures can increase rate of degradation in vitro (Colombatto et al., 2000a). However, the complexity of natural forage makes it difficult to determine what fractions are most affected by enzyme treatment. The use of pure substrates (e.g. cellulose and xylan), provides a way of evaluating enzyme mode of action. Therefore, the present study examined the effects of pre-treating avicel and xylan with an enzyme mixture (EM) on a) the reducing sugars produced during treatment before rumen fermentation and b) the gas production profiles during in vitro rumen fermentation using the Reading Pressure Technique (RPT) (Mauricio et al., 1999).

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Colombatto, D; Mould, F L; Bhat, M K & Owen, E. 2000a. Effects of adding fibrolytic enzymes to alfalfa stems at start of incubation, on the degradation of dry matter and fibre using the ANKOM fermentation system. Proceedings of the 3rd European Symposium on Feed Enzymes. p 4849.
Colombatto, D; Mould, F L; Bhat, M K & Owen, E. 2000b. Biochemical and in vitro assessment of six enzyme preparations as potential feed additives. Proceedings of BSAS 2000, p 54.
Mauricio, R.M.; Mould, F. L.; Owen, E.; Channa, K. S. and Theodorou, M. K. 1999. A semi-automated in vitro gas production technique for ruminant feedstuff evaluation. Animal Feed Science and Technology, 79: 321330.

Fibrolytic enzymes increase the hydrolysis and rate of fermentation of pure substrates in vitro

  • D. Colombatto (a1) (a2), F. L. Mould (a1), M. K. Bhat (a2) and E. Owen (a1)

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