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Exposure of bovine zygotes to serum in a two-phase in vitro culture system adversely affects cleavage rate and cell number of embryos produced

Published online by Cambridge University Press:  20 November 2017

M. Kuran ,
T.G. McEvoy ,
K.D. Sinclair ,
P.J. Broadbent  and
J.J. Robinson
M. Kuran
Affiliation:
Scottish Agricultural College, Animal Biology Division, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, UK
T.G. McEvoy
Affiliation:
Scottish Agricultural College, Animal Biology Division, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, UK
K.D. Sinclair
Affiliation:
Scottish Agricultural College, Animal Biology Division, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, UK
P.J. Broadbent
Affiliation:
Scottish Agricultural College, Animal Biology Division, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, UK
J.J. Robinson
Affiliation:
Scottish Agricultural College, Animal Biology Division, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, UK
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Extract

The number of cells in bovine and ovine embryos is reduced when steer serum which can generate high concentrations of ammonia in vitro is included in the culture system (Kuran et al., 1998). Moreover, production of embryos in synthetic oviduct fluid (SOF) medium supplemented with this serum causes fetal oversize (Sinclair et al., 1998). The present study investigated the effect of the same high ammonia-producing steer serum in a two-phase culture system on bovine embryo development and blastocyst cell numbers.

The experimental design was a 2 x 2 factorial with 5 replicates and involved a total of 928 bovine oocytes that were matured and fertilized in vitro (IVF = Day 0). Presumptive zygotes were cultured in SOF supplemented with 10% v/v steer serum (SOF+SS; n=461) or with amino acids plus 0.4% w/v crystalline BSA (SOFaaBSA; n=467) for an initial period of 48 h following IVF.

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Information
Proceedings of the British Society of Animal Science , Volume 1999 , 1999 , pp. 61
Copyright
Copyright © The British Society of Animal Science 1999

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References

Kuran, M., Sinclair, K.D., Staines, M.E. and McEvoy, T.G. 1998. The effect of culture media on the development and cell number of bovine and ovine embryos. In Gametes: Development and Function (Lauria, A., Gandolfi, F., Enne, G. and Gianaroli, L., editors); Serono Symposia, Italy. p.553.Google Scholar
Sinclair, K.D., Maxfield, E.K., McEvoy, T.G., Carolan, C., Robinson, J.J. and Broadbent, P.J. 1998. Conceptus growth and development following in vitro culture of ovine embryos in media supplemented with bovine sera. Theriogenology 49: 218.CrossRefGoogle Scholar