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Estimation of protein fermentation in the colon of pigs with the gas production technique

Published online by Cambridge University Press:  23 November 2017

J.W. Cone ,
A.W. Jongbloed ,
A.H. van Gelder  and
L. de Lange
J.W. Cone*
Affiliation:
Animal Sciences Group, WUR., P.O., Box 65, NL-8200 AB Lelystad, The Netherlands
A.W. Jongbloed
Affiliation:
Animal Sciences Group, WUR., P.O., Box 65, NL-8200 AB Lelystad, The Netherlands
A.H. van Gelder
Affiliation:
Animal Sciences Group, WUR., P.O., Box 65, NL-8200 AB Lelystad, The Netherlands
L. de Lange
Affiliation:
De Heus Brokking Koudijs B.V., P.O. Box 396, 6710 BJ Ede, The Netherland
*
Email: john.cone@wur.nl
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Extract

Techniques to determine N availability and fermentation characteristics of protein in the hindgut of pigs are not available. The gas production technique (Cone et al., 1996) determines fermentation characteristics of OM and can also be used after pre-digestion of the samples with pepsin and pancreatic enzymes to determine fermentation characteristics of OM in the colon of pigs (Becker et al., 2003). The technique can be adapted to obtain gas production profiles reflecting the fermentation of protein (N availability). To achieve this, incubations have to be done with an excess of fast fermentable carbohydrates, in an N-free environment making N the limiting factor for microbial growth depending on the availability of N from the feed samples. The aim of this study was to investigate the possibilities to use the gas production technique to determine the fermentation characteristics of protein in the hindgut of pigs.

Type
Poster Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2005 , 2005 , pp. 93
Copyright
Copyright © The British Society of Animal Science 2005

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References

Cone., J.W., Van Gelder., A.H., Visscher., G.J.W., and Oudshoorn, L. 1996. Influence of rumen fluid and substrate concentration on fermentation kinetics measured with a fully automated time related gas production apparatus. Animal Feed Science and Technology 61: 113–128.Google Scholar
Becker., P.M., Van Gelder., A.H., Van Wikselaar., P.G., Jongbloed., A.W., and Cone., J.W., 2003. Carbon balances for in vitro digestion and fermentation of potential roughages for pregnant sows. Animal Feed Science and Technology 110: 159–174.CrossRefGoogle Scholar