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Effects of incubation fluid pH and fibrolytic enzymes on the in vitro fermentation of pure substrates, assessed using the Reading Pressure Technique (RPT)

  • D. Colombatto (a1) (a2), F. L. Mould (a1), M. K. Bhat (a2) and E. Owen (a1)

Extract

Modern feeding practices often lead to ruminal conditions being sub-optimal for fibre digestion. It has been speculated that fibrolytic enzymes, which usually show optimum activity at pH values below 6.0, may be of benefit when applied to diets of high producing animals. This study used a commercial enzyme mixture (EM), already identified as effective; to investigate its optimum pH range with respect to activity and its impact on the fermentation profiles of pure substrates, under differing pH conditions.

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Colombatto, D, Mould, F L, Bhat, M K & Owen, E. 2000. Biochemical and in vitro assessment of six enzyme preparations as potential feed additives. Proceedings of the British Society of Animal Science, Page 54.
Mauricio, R M, Mould, F L, Dhanoa, M S, Owen, E, Channa, K S & Theodorou, M K. 1999. A semi-automated in vitro gas production technique for ruminant feedstuff evaluation. Animal Feed Science And Technology 79: 321330.
Wood, T M & Bhat, M K. 1988. Methods for measuring cellulase activities. Methods in Enzymology. Wood, W.A. and Kellogg, S.T. (Eds.), Vol. 160: 87112. Academic Press. London, UK.

Effects of incubation fluid pH and fibrolytic enzymes on the in vitro fermentation of pure substrates, assessed using the Reading Pressure Technique (RPT)

  • D. Colombatto (a1) (a2), F. L. Mould (a1), M. K. Bhat (a2) and E. Owen (a1)

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