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Effective ruminal protection of dietary polyunsaturated fatty acids (PUFA) is very useful in helping to reduce microbial biohydrogenation of PUFA and results in major improvements in the ratio of polyunsaturated:saturated fatty acids (P:S) in beef muscle (Scollan et al., 2003). However, in that study the protected lipid study (PLS) used which consisted of soya beans, linseed and sunflower oils mixed to give a 2.4:1 ratio of 18:2n -6:18:3n -3, was less successful in improving the n -6:n -3 ratio in beef muscle. This study reports the effects of including in the diet a PLS with a lower ratio of 18:2n -6:18:3n -3 on the fatty acid composition of the m. longissimus.