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The effects of amino acid supply on the sensitivity of IGF-1 secretion to growth hormone stimulation in ovine hepatocytes

Published online by Cambridge University Press:  20 November 2017

N.M. Wheelhouse ,
D.G. Hazlerigg ,
J.C. MacRae  and
M.A. Lomax
N.M. Wheelhouse
Affiliation:
Department of Agriculture, University of Aberdeen, 581 King Street Aberdeen, AB24 5UA, UK
D.G. Hazlerigg
Affiliation:
Department of Agriculture, University of Aberdeen, 581 King Street Aberdeen, AB24 5UA, UK
J.C. MacRae
Affiliation:
Rowett Research Institute, Bucksburn, Aberdeen, AB21 9SB, UK
M.A. Lomax
Affiliation:
Department of Agriculture, University of Aberdeen, 581 King Street Aberdeen, AB24 5UA, UK
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Extract

Many of the anabolic effects of growth hormone (GH), including promotion of lean tissue growth, are mediated by the actions of insulin-like growth factor-1 (IGF-1) (Gluckman et al, 1987). It is known that the response of hepatic IGF-1 synthesis to GH may be modulated by alterations in levels of feeding or changes in protein supply (Pell et al, 1993). The aim of the current study was to develop an in vitro model to characterise interactions between amino acid supply and GH on hepatic IGF-1 release in ruminants.

Ovine hepatocyes were prepared by a modification of a published method (Luo et al, 1992) from the median lobe of livers removed immediately after slaughter from sheep killed at a local abattoir. Free amino acid concentrations in test media were 5x, 1x and 0.2x physiological concentrations based upon in vivo ovine portal venous blood data (Lobley et al, 1995).

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Information
Proceedings of the British Society of Animal Science , Volume 1999 , 1999 , pp. 160
Copyright
Copyright © The British Society of Animal Science 1999

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References

Gluckman, P.D., Breier, B.H. and Davis, S.R. 1987. Physiology of the somatotropic axis with particular reference to the ruminant. Journal of Dairy Science 70: 442–466.Google Scholar
Lobley, G.E., Connell, A., Lomax, M.A., Brown, D.S., Milne, E., Calder, A.G. and Farningham, D.A.H. 1995. Hepatic detoxification of ammonia in the ovine liver; possible consequences for amino acid catabolism. British Journal of Nutrition 73: 667–685.Google Scholar
Luo, Q.J., MacRae, J.C. and Scislowski, P.W.D. 1993. Characterization of sheep hepatocytes in primary culture. International Journal of Biochemistry 25: 719–723.Google Scholar
Pell, JM, Saunder, & Gilmour, RS 1993 Differential regulation of transcription initiation from insulin-like growth factor-I (IGF-I) leader exons and of tissue IGF-I expression in response to changed growth hormone and nutritional status in sheep. Endocrinology 132: 1797–1807.CrossRefGoogle ScholarPubMed