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Effect of reducing the interval of recording from three to one hour on gas production profiles in the semi-automated technique using a pressure transducer

Published online by Cambridge University Press:  20 November 2017

R. M. Mauricio
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 236, Reading RG6 6AT
F. L. Mould
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 236, Reading RG6 6AT
E. Owen
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 236, Reading RG6 6AT
M.S. Dhanoa
Affiliation:
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3EB
M.K. Theodorou
Affiliation:
Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth SY23 3EB
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Extract

Mauricio et al.(1997) reported that faeces as an inoculum in the gas production produced similar results to rumen liquor in terms of total gas production and OM digestibility but a greater initial lag phase was observed. Where a mathematical description is to be used to develop the gas production profiles an accurate description of the lag phase (the period prior to degradation during which multiplication and attachment of the micro-organisms occur) must be obtained. For this to be achieved when a large number of samples are to be examined, measurement of gas production has to be achieved rapidly; this is possible using a semi-automated technique (Mauricio et al., 1998). This study evaluated the effect of recording gas production using a manual (syringe) technique, with a standard 3 h interval, and at 1h and 3 h intervals using a semi-automated system.

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Copyright
Copyright © The British Society of Animal Science 1999

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References

France, J., Dhanoa, M.S., Theodorou, M.K., Lister, S.J., Davies, S.J. and Isac, D. 1993. A model to interpret gas accumulation profiles with in vitro degradation of ruminant feeds. Journal of Theoretical Biology 163:99111.CrossRefGoogle Scholar
Mauricio, R.M., Dhanoa, M.S., Owen, E., Channa, K.S., Mould, F.L., Theodorou, M.K. 1998. Semi-automation of in vitro gas production technique using a pressure transducer. Proceedings of the British Society of Animal Science, 23-25th March 1998.Google Scholar
Theodorou, M.K., Williams, B.A., Dhanoa, M.S., McAllan, A.B. and France, J. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology 48:185197.CrossRefGoogle Scholar