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Effect of dietary fish and soya oil supplementation on ∆-9 desaturase relative gene expression in muscle of beef cattle

Published online by Cambridge University Press:  23 November 2017

S.M. Waters ,
J. Kelly ,
P. O’Boyle ,
A.P. Moloney  and
D.A. Kenny
S.M. Waters*
Affiliation:
Teagasc, Animal Production Research Centre, Mellows Campus, Athenry, Co. Galway, Ireland
J. Kelly
Affiliation:
School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland
P. O’Boyle
Affiliation:
Teagasc, Animal Production Research Centre, Mellows Campus, Athenry, Co. Galway, Ireland
A.P. Moloney
Affiliation:
Teagasc, Beef Cattle Research Centre, Grange, Dunsany, Co. Meath, Ireland
D.A. Kenny
Affiliation:
School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland
*
Email:Sinead.Waters@teagasc.ie
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Extract

Supplementation of cattle diets with a blend of oils rich in omega-3 polyunsaturated fatty acids (ω-3 PUFA) and linoleic acid have a synergistic effect on the accumulation of ruminal and tissue concentrations of vaccenic acid (VA), the main substrate for ∆-9 desaturase catalysed de novo tissue synthesis of the cis 9, trans 11 isomer of conjugated linoleic acid (CLA). This dietary strategy translates into increases in milk concentrations of CLA in dairy cows, however, CLA concentrations in the muscle of beef animals have not always been increased (Kenny et al., 2007). Studies with in-vitro mammary cell lines (Keating et al., 2006) and mice (Ntambi et al., 1999) suggest that ω-3 PUFA may inhibit expression of ∆-9 desaturase. Furthermore, the promoter regions of the bovine ∆-9 desaturase gene have been shown to contain a conserved PUFA response region (Keating et al., 2005) and this has been suggested as a mechanism through which ω-3 PUFA may modulate ∆-9 desaturase expression in bovine muscle. Currently there is no evidence to support this hypothesis. The objective of this study, therefore, was to determine the influence of ω-3 PUFA dietary supplementation on the expression of ∆-9 desaturase gene in bovine muscle.

Type
Theatre Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2007 , April 2007 , pp. 12
Copyright
Copyright © The British Society of Animal Science 2007

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References

Keating, A.F., Stanton, C., Murphy, J.J., Smith, T.J., Ross, R.P., Cairns, M.T. 2005. Isolation and characterisation of the bovine steroyl-CoA desaturase promoter and analysis of polymorphism in the promoter region in dairy cows. Mammalian Genome 16:184–193.CrossRefGoogle Scholar
Keating, A.F., Kennelly, J.J., Zhao, F.Q. 2006. Characterisation and regulation of the bovine stearoyl-CoA desaturase gene promoter. Biochemica Biophysica Research Commun. 344: 233–240.Google Scholar
Kenny, D. A., Kelly, J.P., Monahan, F.J., Moloney, A.P. 2007. Effect of dietary fish and soya oil on muscle fatty acid concentrations and oxidative lipid stability in beef cattle. Proceedings of the British Society Animal Science.CrossRefGoogle Scholar
Ntambi, J.M. 1999. Regulation of stearoyl-CoA desaturase by polyunsaturated fatty acids and cholesterol. Journal Lipid Research. 40:1549–1558.Google Scholar