There have been numerous studies of laterality (handedness, sidedness or lateralised motor behaviour) involving several species including humans, primates, dogs, cats, rodents, whales and horses. In humans for example, population bias of the order of 93% for right-handedness has been reported from studies of more than 5000 years of art work, which appears to be unaffected by culture or geographic location. It has long been accepted that the majority of horses are lateralised (to varying degrees) and training programmes have been designed to ‘straighten’ the lateralised competition horse, (Klimke, 2003). The specific cause of laterality in any species still remains open to some degree of debate and theories of genetic predisposition, environmental influences and combinations of both have been proposed. While both environmental and genetic models have been suggested with regard to the aetiology of lateralised behaviour, mapping of any specific locus, which could be unequivocally attributed to lateralised behaviour is as of yet incomplete. There is evidence suggesting a relationship between facial hair whorls and side preferences from studies in cattle, Tanner et al., (1994). Dalin et al., (1985) reported that laterality and asymmetry in equine motor behaviour may negatively affect locomotion and subsequently compromise competitive performance. The objective of this study was to explore the possibility of predicting the direction of lateral bias in the horse from observations of the direction of facial hair whorl rotation.