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A comparison of solvents for extraction of polyphenolic compounds in tree leaves

Published online by Cambridge University Press:  20 November 2017

CM Capetillo Leal
Affiliation:
Fac. Vet. Med. & Anim. Sci. – Univ. of Yucatan, México. Apdo. 4-116 Itzimna, Mérida, Yucatán, 97100, México
R. Reyes Ramírez
Affiliation:
Fac. Vet. Med. & Anim. Sci. – Univ. of Yucatan, México. Apdo. 4-116 Itzimna, Mérida, Yucatán, 97100, México
CA Sandoval-Castro
Affiliation:
Fac. Vet. Med. & Anim. Sci. – Univ. of Yucatan, México. Apdo. 4-116 Itzimna, Mérida, Yucatán, 97100, México
D. Camacho Morfin
Affiliation:
Fac. Vet. Med. & Anim. Sci. – Univ. of Yucatan, México. Apdo. 4-116 Itzimna, Mérida, Yucatán, 97100, México
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Extract

The occurrence of polyphenolics compounds in forage is of nutritional significance due to its protein binding capacity which can affect protein digestion (Woodward and Reed, 1989). They also have a role in controlling rates of decomposition and N mineralization of plant residues (Swift et al., 1979). The Prussian-blue assay (Price and Butler, 1977) is widely use for quantitative measurement of polyphenol content of plant materials due to the easiness it can be performed. Polyphenols are polar compound and literature report extractions with, methanol, ethanol or acetone as usual solvents. Although it is known that no single protocol is optimum for all samples. No comparison was found between solvents on a wide range of material to assess if results might be correlated. The objective of the present work was to compare the efficiency of four solvents for polyphenol extraction.

Type
Poster Presentations
Copyright
Copyright © The British Society of Animal Science 2002

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