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Analysis of Kappa casein polymorphism in three Iranian native cattle and Holstein breeds by PCR-RFLP

Published online by Cambridge University Press:  23 November 2017

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Kappa Casein (◻-casein) is a protein in mammalian milk whose two common genetic variants of A and B are well known. The A variant has a Thr and Asp at positions 136 and 148, while the B variant has Ile and Ala in these positions. Difference at position 148 can be detected by PCR-RFLP (Medrano and Agiular-Cordova, 1990). These two alleles determine great differences in milk, casein, protein and fat yields as well as cheese making properties such as coagulation time and curd firmness, with a superiority for cheese production of the κ-casein BB compared to κ-casein AA milk (Lodes et al., 1996). Several internationally studies have been focused on determining the frequency distribution of the various milk protein alleles in different animals. It is important to study not only the major breeds but also those with small animal numbers (Erhardt, 1996). The aim of this study was to estimate the allelic frequency in polymorphic site of exon 4 of κ-casein gene in three Iranian native and Holstein cattle.

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Poster presentations
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Copyright © 2016 The American Society of International Law

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References

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