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Comparison of inocula from sheep and cattle for the in vitro gas production technique under tropical conditions

Published online by Cambridge University Press:  20 November 2017

I.C.S. Bueno
Affiliation:
Animal Nutrition Lab, CENA/USP, Caixa Postal 96, CEP 13400-970, Piracicaba, São Paulo, Brazil
A.L. Abdalla
Affiliation:
Animal Nutrition Lab, CENA/USP, Caixa Postal 96, CEP 13400-970, Piracicaba, São Paulo, Brazil
S.L.S. Cabral Filho
Affiliation:
Animal Nutrition Lab, CENA/USP, Caixa Postal 96, CEP 13400-970, Piracicaba, São Paulo, Brazil
D.M.S.S. Vitti
Affiliation:
Animal Nutrition Lab, CENA/USP, Caixa Postal 96, CEP 13400-970, Piracicaba, São Paulo, Brazil
E. Owen
Affiliation:
Animal Nutrition Lab, CENA/USP, Caixa Postal 96, CEP 13400-970, Piracicaba, São Paulo, Brazil
R.M. Mauricio
Affiliation:
Animal Nutrition Lab, CENA/USP, Caixa Postal 96, CEP 13400-970, Piracicaba, São Paulo, Brazil
I. Givens
Affiliation:
Animal Nutrition Lab, CENA/USP, Caixa Postal 96, CEP 13400-970, Piracicaba, São Paulo, Brazil
J.D. Sutton
Affiliation:
Animal Nutrition Lab, CENA/USP, Caixa Postal 96, CEP 13400-970, Piracicaba, São Paulo, Brazil
F. L. Mould
Affiliation:
Animal Nutrition Lab, CENA/USP, Caixa Postal 96, CEP 13400-970, Piracicaba, São Paulo, Brazil
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Extract

The use of small ruminants, such as sheep, in metabolism studies is more convenient as handling problems are reduced and their maintenance costs are lower, in comparison with cattle. However in vivo digestibility estimates obtained at maintenance are known to differ between these two species. With the increased use of in vitro gas production techniques, to evaluate ruminant feedingstuffs, it is of great importance to identify whether the species from which the rumen fluid inoculum is obtained has a significant influence on the results obtained.

Rumen fluid samples were obtained from a non-lactating Holstein cow (C) and six wether sheep (S) offered the same diet (80 % tropical grass and 20 % dairy concentrate) and prepared so as to have similar dry matter (DM) contents and therefore potentially the microbial mass. Nine substrates (two tropical grasses 1-2, tropical alfalfa 3, barley straw 4, and five temperate grasses 5-9) were examined.

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Copyright
Copyright © The British Society of Animal Science 1999

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References

France, J., Dhanoa, M.S., Theodorou, M.K., Lister, S.J., Davies, S.J. and Isac, D. 1993. A model to interpret gas accumulation profiles with in vitro degradation of ruminant feeds. Journal of Theoretical Biology 163:99111.CrossRefGoogle Scholar
Theodorou, M.K., Williams, B.A., Dhanoa, M.S., McAllan, A.B. and France, J. 1994. A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Animal Feed Science and Technology 48:185197.CrossRefGoogle Scholar
Mauricio, R. M., Dhanoa, M.S., Owen, E., Channa, K.S., Mould, F.L.; Theodorou, M.K. Semi-automation of the in vitro gas production technique using a pressure transducer. Poster no. 70 Annual Meeting, British Society of Animal Science, 23-25th March 1998, Scarborough, EnglandGoogle Scholar

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