Hostname: page-component-8448b6f56d-gtxcr Total loading time: 0 Render date: 2024-04-19T21:55:05.769Z Has data issue: false hasContentIssue false
  • English
  • Français

Screening for Eruca genotypes tolerant to polyethylene glycol-simulated drought stress based on the principal component and cluster analyses of seed germination and early seedling growth

Published online by Cambridge University Press:  10 November 2015

Banglian Huang ,
Jianjie Su ,
Guangyu Zhang ,
Xiaoqing Luo ,
Huan Wang ,
Yue Gao ,
Guilin Ma ,
Jingyu Wang ,
Detian Cai  and
Xuekun Zhang
...Show all authors
Banglian Huang
Affiliation:
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Science, Hubei University, Wuhan430062, People's Republic of China
Jianjie Su
Affiliation:
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Science, Hubei University, Wuhan430062, People's Republic of China
Guangyu Zhang
Affiliation:
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Science, Hubei University, Wuhan430062, People's Republic of China
Xiaoqing Luo
Affiliation:
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Science, Hubei University, Wuhan430062, People's Republic of China
Huan Wang
Affiliation:
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Science, Hubei University, Wuhan430062, People's Republic of China
Yue Gao
Affiliation:
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Science, Hubei University, Wuhan430062, People's Republic of China
Guilin Ma
Affiliation:
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Science, Hubei University, Wuhan430062, People's Republic of China
Jingyu Wang
Affiliation:
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Science, Hubei University, Wuhan430062, People's Republic of China
Detian Cai
Affiliation:
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Science, Hubei University, Wuhan430062, People's Republic of China
Xuekun Zhang*
Affiliation:
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan430062, People's Republic of China
Bangquan Huang*
Affiliation:
Hubei Collaborative Innovation Center for Green Transformation of Bio-Resources, College of Life Science, Hubei University, Wuhan430062, People's Republic of China
*
*Corresponding authors: E-mail: zhang.xk@139.com; huangbangquan@163.com
*Corresponding authors: E-mail: zhang.xk@139.com; huangbangquan@163.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Water deficit is an environmental factor that constrains crops to express their ecophysiological potential and causes crop yield reduction. Eruca vesicaria has been reported to be one of the most drought-tolerant species in Cruciferae. In this study, polyethylene glycol-simulated drought tolerance was evaluated in one line of Brassica carinata, one line of Brassica napus and 249 Eruca lines based on the principal component analysis (PCA) and unweighted pair-group arithmetic average (UPGMA) cluster analysis. The PCA based on eight drought tolerance indices indicated that the first three components accounted for 85.46% of the total variation, with principal component (PC) 1 accounting for 43.89%, PC2 for 27.85% and PC3 for 13.73% of the total variation. The UPGMA cluster analysis indicated that B. napus cultivar Zhongshuang 9 and Eruca lines could be clustered into five major groups, with group 1 being, in general, drought sensitive, group 2 being slightly–medium drought tolerant, group 3 being drought tolerant, group 4 being highly drought sensitive and group 5 being highly drought tolerant. B. carinata cultivar XB1, as an outstander, showed high drought sensitivity. The UPGMA cluster dendrogram provides a good representation of the similarity matrix (r= 0.68). The drought-tolerant Eruca materials obtained in this study will be valuable for genetic improvement not only in Eruca itself, but also in Brassica crops since they are drought-tolerant lines from a drought-tolerant species.

Keywords

cluster analysisearly seedling growthErucaPEG-simulated drought toleranceprincipal component analysisseed germination
Type
Research Article
Information
Plant Genetic Resources , Volume 15 , Issue 2 , April 2017 , pp. 187 - 193
Copyright
Copyright © NIAB 2015 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alvin, CR (2002) Methods of Multivariate Analysis. New York: John Wiley & Son.Google Scholar
Ashraf, M (1994) Organic substances responsible for salt tolerance in Eruca sativa . Biologia Plantarum 36: 255–259.Google Scholar
Ashraf, M, Ozturk, M and Athar, HR (2009) Salinity and Water Stress. Improving Crop Efficiency. Berlin, Germany: Springer.Google Scholar
Blum, A (1988) Plant Breeding for Stress Environments. Boca Raton, FL: CRC Press.Google Scholar
Bouslama, M and Schapaugh, WT (1984) Stress tolerance in soybean. I. Evaluation of three screening techniques for heat and drought tolerance. Crop Science 24: 933–937.Google Scholar
Budak, H, Kantar, M and Kurtoglu, KY (2013) Drought tolerance in modern and wild wheat. The Scientific World Journal 2013: 1–16.Google Scholar
Ellis, RH and Roberts, EH (1980) Towards a rational basis for testing seed quality. In: Hebblethwaite, PD (ed.) Seed Production. London: Butterworths, pp. 605–635.Google Scholar
Gomez, F, Oliva, MA, Mielke, MS, de Almeida, AAF, Leita, HG and Aquino, LA (2008) Photosynthetic limitations in leaves of young Brazilian Green Dwarf coconut (Cocos nucifera L. “nana” palm under well-watered conditions or recovering from drought stress. Environmental and Experimental Botany 62: 195–204.Google Scholar
Grzesiak, S (2001) Genotypic variation between maize (Zea mays L.) single cross hybrids in response to drought stress. Acta Physiologiae Plantarum 23: 437–451.CrossRefGoogle Scholar
Grzesiak, S, Iijima, M, Kono, Y and Yamauehi, A (1997) Differences in drought tolerance between cultivars of field bean and field pea: a comparison of drought-resistance and drought-sensitive cultivars. Acta Physiologiae Plantarum 19: 349–357.Google Scholar
Grzesiak, S, Grzesiak, MT, Filek, W and Stabryta, J (2003) Evaluation of physiological screening tests for breeding drought resistant triticale ((Triticosecale Wittmack). Acta Physiologiae Plantarum 25: 29–37.Google Scholar
Hardegree, SP and Emmerich, WE (1990) Effect of polyethylene glycol exclusion on the water potential of solution-saturated filter paper. Plant Physiology 92: 462–465.Google Scholar
Hegde, VS and Mishra, SK (2009) Landraces of cowpea Vigna unguiculata (L.) Walp., as potential sources of genes for unique characters in breeding. Genetic Resource and Crop Evolution 56: 615–627.Google Scholar
Huang, B, Liao, S, Cheng, C, Ye, X, Luo, M, Li, Z, Cai, D, Wu, W and Huang, B (2014) Variation, correlation, regression and path analyses in Eruca sativa Mill. African Journal of Agricultural Research 9: 3744–3750.Google Scholar
Jones, HG and Corlett, JE (1992) Current topics in drought physiology. Journal of Agricultural Science 119: 291–296.CrossRefGoogle Scholar
Khan, MA and Ungar, IA (1984) The effect of salinity and temperature on the germination of polymorphic seeds and growth of Atriplex triangularis Willd. American Journal of Botany 71: 481–489.Google Scholar
Kron, AP, Souza, GM and Ribeiro, RV (2008) Water deficiency at different developmental stages of Glycine max can improve drought tolerance. Bragantia 67: 43–49.Google Scholar
Kumar, B, Abdel-Ghani, AH, Pace, J, Reyes-Matamoros, J, Hochholdinger, F and Lübberstedt, T (2014) Association analysis of single nucleotide polymorphisms in candidate genes with root traits in maize (Zea mays L.) seedlings. Plant Science 224: 9–19.Google Scholar
Larsson, S and Gómy, AG (1988) Grain yield and drought resistance indices of oat cultivars in field rain shelter and laboratory experiments. Journal of Agronomy and Crop Science 161: 277–286.Google Scholar
Lawlor, DW (1970) Absorption of polyethylene glycols by plants and their effects on plant growth. New Phytologist 69: 501–513.Google Scholar
Li, Z, Yang, CJ, Zhang, XK, Zou, CS, Cheng, Y, Zheng, PY and Li, GY (2008) Evaluation of drought tolerance in rapeseed (Brassica napus L.) during germination under PEG6000 stress. Chinese Journal of Oil Crop Science 30: 438–442.Google Scholar
Mantel, N (1967) The detection of disease clustering and a generalized regression approach. Cancer Research 27: 175–178.Google Scholar
Peleg, Z, Fahima, T, Abbo, S, Krugman, T, Nevo, E, Yakir, D and Saranga, Y (2005) Genetic diversity for drought resistance in wild emmer wheat and its ecogeographical associations. Plant, Cell & Environment 28: 176–191.Google Scholar
Perez-Alfocea, F, Estan, MT, Caro, M and Guerrier, G (1993) Osmotic adjustment in Lycopersicon esculentum and L. penneli under NaCl and polyethylene glycol 6000 iso-osmotic stress. Physiology Plantarum 87: 493–498.Google Scholar
Prakash, S and Bhat, SR (2007) Contribution of wild crucifers in Brassica improvement: past accomplishment and future perspectives. Proceedings of GCIRC 12th International Rapeseed Congress 1: 213–215.Google Scholar
Razavi, F, Keyser, ED, Riek, JD and Van Labeke, MC (2011) A method for testing drought tolerance in Fragaria based on fast screening for water deficit response and use of associated AFLP and EST candidate gene markers. Euphytica 180: 385–409.Google Scholar
Richards, RA and Thurling, N (1978) Variation between and within species of rapeseed (Brassica campestris and B. napus) in response to drought stress. I. Sensitivity at different stages of development. Australian Journal of Agricultural Research 29: 469–477.Google Scholar
Rohlf, FJ (2000) NTSYSpc Numerical Taxonomy and Multivariate Analysis System, Version 2.10m. Setauket: Exeter Software.Google Scholar
Shamimi, F, Saqlan, SM, Athar, HUR and Waheed, A (2014) Screening and selection of tomato genotypes/cultivars for drought tolerance using multivariate analysis. Pakistan Journal Botany 46: 1165–1178.Google Scholar
Souza, GM and Cardoso, VJM (2003) Toward a hierarchical concept of plant stress. Israel Journal of Plant Sciences 51: 29–37.CrossRefGoogle Scholar
Sun, WC, Yang, Q, Zhang, J, Zhang, T and Yun, Z (1999) Assessment on drought tolerance of Eruca sativa genotypes from northwestern China. Proceedings of the 10th International Rapeseed Congress, Canberra, Australia, pp. 217..Google Scholar
Sun, WC, Pan, QY, Liu, ZG, Meng, YX, Zhang, T, Wang, HL and Zeng, XC (2004) Genetic resources of oilseed Brassica and related species in Gansu Province, China. Plant Genetic Resource: Characterization and Utilization 2: 167–173.Google Scholar
Warwick, SI, Gukel, RK, Gomez-Campo, C and James, T (2007) Genetic variation in Eruca vesicaria (L.) Cav. Plant Genetic Resource: Characterization and Utilization 5: 142–153.Google Scholar
Winter, SR, Musick, JT and Porter, KB (1988) Evaluation of screening techniques for breeding drought-resistant winter wheat. Crop Science 28: 512–516.Google Scholar
Worbes, M, Blanchart, S and Fichtler, E (2013) Relations between water balance, wood traits and phenological behavior of tree species from a tropical dry forest in Costa Rica – a multifactorial study. Tree Physiology 33: 527–536.Google Scholar
Xie, XY, Zhang, X and Zhang, B (2013) Evaluation of Drought Resistance and Analysis of Variation of Relevant Parameters at Seedling Stage of Rapeseed (Brassica napus L.). Scientia Agricultura Sinica 46: 476–485.Google Scholar
Zgallai, H, Steppe, K and Lemeur, R (2005) Photosynthetic, physiological and biochemical responses of tomato plants to polyethylene glycol-induced water deficit. Journal of Integrative Plant Biology 47: 1470–1478.Google Scholar
Zhi, Y, Deng, Z, Luo, M, Ding, W, Hu, Y, Deng, J, Li, Y, Zhao, Y, Zhang, X, Wu, W and Huang, B (2015) Influence of heavy metals on seed germination and early seedling growth in Eruca sativa Mill. American Journal of Plant Sciences 6: 582–590.Google Scholar
Supplementary material: Image

Huang supplementary material

Figure S1-1

Download Huang supplementary material(Image)
Image 102.4 KB
Supplementary material: Image

Huang supplementary material

Figure S1-2

Download Huang supplementary material(Image)
Image 654.2 KB
Supplementary material: Image

Huang supplementary material

Figure S1-3,4,5

Download Huang supplementary material(Image)
Image 64.1 KB
Supplementary material: File

Huang supplementary material

Table S1

Download Huang supplementary material(File)
File 414.7 KB