Skip to main content Accessibility help
×
Home

Microsatellite markers for common lilac (Syringa vulgaris L.)

  • Inka Juntheikki-Palovaara (a1), Kristiina Antonius (a2), Leena Lindén (a1) and Helena Korpelainen (a1)

Abstract

Common lilac is a popular landscape plant with nearly 2000 cultivars. In the present study, nine novel polymorphic microsatellite markers were developed for common lilac using genome screening with inter-simple sequence repeat primers. Each of the nine markers revealed two to five alleles. The markers were used for genotyping of 75 common lilac shrubs, including 17 accessions that represented named cultivars. A dendrogram, based on the allele profiles of the shrubs, was generally in agreement with the grouping made on the basis of morphological traits, as well as with the pedigree information available. The novel markers appear valuable for differentiation between common lilac cultivars.

Copyright

Corresponding author

* Corresponding author. E-mail: leena.linden@helsinki.fi

References

Hide All
Doyle, JJ and Doyle, JL (1990) Isolation of plant DNA from fresh tissue. Focus 12: 1315.
De La Rosa, R, James, CM and Tobutt, KR (2002) Isolation and characterization of polymorphic microsatellites in olive (Olea europaea L.) and their transferability to other genera in the Oleaceae. Molecular Ecology Notes 2: 265267.
Fiala, JL and Vrugtman, F (2008) Lilacs. A Gardener's Encyclopedia. 2nd revised edn.Portland: Timber Press.
Harbourne, ME, Douglas, GC, Waldren, S and Hodkinson, TR (2005) Characterization and primer development for amplification of chloroplast microsatellite regions of Fraxinus excelsior. Journal of Plant Research 118: 339341.
Kodama, K, Yamada, T and Maki, M (2008) Development and characterization of 10 microsatellite markers for the semi-evergreen tree species, Ligustrum ovalifolium (Oleaceae). Molecular Ecology Resources 8: 10081010.
Korpelainen, H, Kostamo, K and Virtanen, V (2007) Microsatellite marker identification using genome screening and restriction–ligation. BioTechniques 42: 479486.
Lindén, L, Hauta-aho, L, Temmes, O and Tegel, S (2010) An inventory of old lilac and crab apple cultivars in the city of Helsinki, Finland. Acta Horticulturae no. 881 2: 10271030.
McKelvey, SD (1928) The Lilac – A Monograph. New York: Macmillan.
Peakall, R and Smouse, P (2006) GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6: 288295.
Rzepka-Plevneš, D, Smolik, M and Tańska, K (2006) Genetic similarity of chosen Syringa species determined by the ISSR-PCR technique. Dendrobiology 56: 6167.
Smolik, M, Andrys, D, Franas, A, Krupa-Malkiewicz, M and Malinowska, K (2010) Polymorphism in Syringa rDNA regions assessed by PCR technique. Dendrobiology 64: 5564.
Tamura, K, Dudley, J, Nei, M and Kumar, S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution 24: 15961599.

Keywords

Type Description Title
WORD
Supplementary materials

Juntheikki-Palovaara Supplementary Material
Table S1-S2

 Word (38 KB)
38 KB

Microsatellite markers for common lilac (Syringa vulgaris L.)

  • Inka Juntheikki-Palovaara (a1), Kristiina Antonius (a2), Leena Lindén (a1) and Helena Korpelainen (a1)

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed