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Genetic variation and association mapping of grain iron and zinc contents in synthetic hexaploid wheat germplasm

Published online by Cambridge University Press:  12 August 2016

Yasir S. A. Gorafi ,
Takayoshi Ishii ,
June-Sik Kim ,
Awad Ahmed Elawad Elbashir  and
Hisashi Tsujimoto
Yasir S. A. Gorafi
Affiliation:
Arid Land Research Center, 1390 Hamasaka, Tottori 680-0001, Japan Agricultural Research Corporation, Wad-Medani, PO Box 126, Sudan
Takayoshi Ishii
Affiliation:
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstraßsse 3, 06466 Gatersleben, Germany
June-Sik Kim
Affiliation:
RIKEN Center for Sustainable Resource Science, Koyadai, Tsukuba, Ibaraki 305-0074, Japan
Awad Ahmed Elawad Elbashir
Affiliation:
Arid Land Research Center, 1390 Hamasaka, Tottori 680-0001, Japan
Hisashi Tsujimoto*
Affiliation:
Arid Land Research Center, 1390 Hamasaka, Tottori 680-0001, Japan
*
*Corresponding author. E-mail: tsujim@alrc.tottori-u.ac.jp
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Abstract

Fe and Zn deficiency are widespread worldwide. As wheat is the primary food for the majority of the world people, producing wheat grains with high mineral content can ameliorate the problem of mineral hunger. However, the genetic variation available for breeders is limited. The aim of this study was to assess the genetic variation in grain Fe and Zn contents in 47 synthetic hexaploid wheats and to identify marker loci associated with Fe and Zn contents. We measured the grain Fe and Zn contents using inductively coupled plasma atomic emission spectroscopy and performed genotyping using SSR markers. The results showed considerable genetic variation for these minerals. We identified three lines with high Fe and Zn contents and six quantitative trait loci of which three were associated with Fe content and the other three with Zn content. The minerals showed positive phenotypic and genotypic correlation and high heritability (>60%). The ratio of the σ2g to the σ2g×e was ≥1 for the two mineral contents indicating that breeding for increasing mineral content within the synthetic lines is possible. The synthetic wheat lines identified in this study are valuable genetic resources, and can be utilized for breeding wheat cultivars with high mineral content.

Keywords

association mappingdroughtgenetic resourcesmineral contentwheat breedingsynthetic wheat
Type
Research Article
Information
Plant Genetic Resources , Volume 16 , Issue 1 , February 2018 , pp. 9 - 17
Copyright
Copyright © NIAB 2016 

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