Skip to main content Accessibility help
×
Home

Genetic diversity and variation analysis of mutant lines derived from γ-ray and chemical mutagen treatments in blackberry (Rubus fruticosus)

  • Jaihyunk Ryu (a1), Bo-Keun Ha (a1) (a2), Dong Sub Kim (a1), Jin-Baek Kim (a1), Sang Hoon Kim (a1), Joon-Woo Ahn (a1), Il Yun Jeong (a1), Han-Jik Jo (a3), Ee-Yup Kim (a3) and Si-Yong Kang (a1)...

Abstract

Blackberry is a fruiting berry species with very high nutrient contents. With the recent increasing consumer demand for blackberries, new sources of germplasm and breeding techniques are required to improve blackberry production. This study was carried out to evaluate the genetic diversity (GD) and relationship among 55 blackberry (Rubus fruticosus) mutants derived from γ-ray treatment (52 lines) and N-methyl-N′-nitrosourea (MNU) treatment (three lines) using an inter-simple sequence repeat marker. A total of 18 bands were amplified with an average of 3.6 bands per primer. Among them, eight bands were identified to be polymorphic with a rate of 44.4%. In addition, the GD information content values were highest in the 60 Gy treatment population and the GD values were higher in the γ-ray treatment populations than in the MNU treatment population. According to a cluster analysis, all the mutant lines can be classified into five categories, and the genetic distance was greatest between the 80 Gy-irradiated population and other populations. These results indicate that mutant lines have high GD and can be effectively utilized for improving blackberry breeding.

Copyright

Corresponding author

* Corresponding author. E-mail: sykang@kaeri.re.kr

References

Hide All
Esselman, EJ, Jianqiang, L, Crawford, DJ, Winduss, JL and Wolfe, AD (1999) Clonal diversity in the rare Calamagrostis porteri ssp. insperata (Poaceae): comparative results for allozymes and random amplified polymorphic DNA (RAPD) and their simple sequence repeat (ISSR) markers. Molecular Ecology 8: 443451.
Fang, G, Hammar, S and Grumet, R (1992) A quick inexpensive method of removing polysaccharides from plant genomic DNA. Biotechniques 13: 5255.
Halvorsen, BL, Carlsen, MH and Phillips, KM (2006) Content of redox-active compounds (i.e., antioxidants) in foods consumed in the United States. The American Journal of Clinical Nutrition 84: 95135.
Kang, EJ, Lee, YM, Sung, SY, Ha, BK, Kim, SH, Kim, DS, Kim, JB and Kang, SY (2013) Analysis of the genetic relationship of gamma-irradiated in vitro mutants derived from standard-type Chrysanthemum cv. Migok. Horticulture, Environment, and Biotechnology 54: 7681.
Marulanda, ML, López, AM and Aguilar, SB (2007) Genetic diversity of wild and cultivated Rubus species in Colombia using AFLP and SSR markers. Crop Breeding and Applied Biotechnology 7: 242252.
Menz, MA, Klein, RR, Unruh, NC, Rooney, WL, Klein, PE and Mullet, JE (2004) Genetic diversity of public inbreds of sorghum determined by mapped AFLP and SSR markers. Crop Science 44: 12361244.
Predieri, S, Bertazza, G and Gennari, F (1998) Pear cv. bartlett mutants selection for high fruit quality: analysis of soluble sugars and organic acids. In: Atti IV Giornate Scientifiche SOI, 1–3 April 1998, Sanremo, Italy , pp. 6566.
Russel, TR, Fuller, JD, Macaulay, M, Hatz, BG, Jahoor, A, Powell, WP and Waugh, R (1997) Direct comparison of level of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs. Theoretical and Applied Genetics 95: 714722.
Thompson, MM (1997) Survey of chromosome number in Rubus (Rosaceae: Rosoideae). Annals of the Missouri Botanical Garden 84: 129165.
Van Harten, AM (1998) Mutation Breeding: Theory and Practical Applications. Cambridge: Cambridge University Press.

Keywords

Type Description Title
WORD
Supplementary materials

Ryu Supplementary Material
Tables S1-S3 and Figure S1

 Word (64 KB)
64 KB

Metrics

Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed