Skip to main content Accessibility help

Genetic diversity analysis of kenaf (Hibiscus cannabinus L.) using AFLP (amplified fragment length polymorphism) markers

  • Rouxlene Coetzee (a1), Liezel Herselman (a1) and Maryke T. Labuschagne (a1)


Nineteen kenaf genotypes from Cuba, Taiwan, the USA, El Salvador, Guatemala, Russia, Spain and Indonesia, and three wild types collected in South Africa were analysed for genetic diversity using AFLP analysis. All could be uniquely distinguished from one another, but only a low level of genetic diversity was present. The most distinct accession, Guatemala 4, was 85% similar to all other accessions. The accessions clustered more or less according to known pedigree and/or origin. Two of the three wild types (Hibiscus cannabinus c and H. cannabinus a) clustered separately from the commercial and Russian accessions. One of the wild types, H. cannabinus b clustered with some of the commercial accessions. Commercial accessions in the first subgroup all originated from central and North America, and surrounding islands (Cuba and El Salvador). The Russian accessions are all grouped together. The second subgroup was the only group that contained accessions from different geographical origins.


Corresponding author

*Corresponding author. E-mail:


Hide All
Baldwin, BS, Hollowell, JE, Mosley, JW and Cossar, RD (2006) Registration of ‘Whitten’ kenaf. Crop Science 46: 988989.
Cheng, Z, Lu, B, Baldwin, BS, Sameshima, K and Chen, J (2002) Comparative studies of genetic diversity in kenaf (Hibiscus cannabinus L.) varieties based on agronomic and RAPD data. Hereditas 136: 231239.
Cheng, Z, Lu, B, Sameshima, K, Fu, D and Chen, J (2004) Identification and genetic relationships of kenaf (Hibiscus cannabinus L.) germplasm revealed by AFLP analysis. Genetic Resources and Crop Evolution 51: 393401.
Cook, CG and Scott, AW (1995) Registration of ‘SF459’ kenaf. Crop Science 35: 1712.
Cook, CG and Scott, AW (2000 a) Registration of ‘Dowling’ kenaf. Crop Science 40: 1831.
Cook, CG and Scott, AW (2000 b) Registration of ‘Gregg’ kenaf. Crop Science 40: 18311832.
Crane, JC and Acuna, JB (1945) Growth and development of kenaf, Hibiscus cannabinus L., with special reference to the fiber content of the stems. Journal of the American Society for Agronomy 37: 352359.
Dempsey, JM (1975) Fiber Crops. Gainesville: The University Presses of Florida.
Deng, L-Q, Li, J-Q and Li, A-Q (1994) Studies on the agronomic characters of kenaf germplasm and their utilisation. China's Fibre Crops 4: 14.
Dice, LR (1945) Measures of the amount of ecologic association between species. Ecology 26: 297302.
Edwards, K, Johnstone, C and Thompson, C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Research 19: 1349.
LeMahieu, PJ, Oplinger, ES and Putnam, DH (1991) Kenaf. Alternative Field Crops Manual. Available at (accessed July 2007)..
Siepe, T, Ventrella, D and Lapenta, E (1997) Evaluation of genetic variability in a collection of Hibiscus cannabinus (L.) and Hibiscus spp (L.). Industrial Crops and Products 6: 343352.
Taylor, CS (1998) Kenaf. Available at (accessed February 2007)..
USDA ARS, National Genetic Resources Program (2004) Germplasm Resources Information Network – (GRIN)[Online Database] National Germplasm Resources Laboratory, Beltsville, MD. Available at
Vos, P, Hogers, R, Bleeker, M, Reijans, M, Van de Lee, T, Hornes, M, Frijters, A, Pot, J, Peleman, J, Kuiper, M and Zabeau, M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23: 44074414.
Wood, I (1998) Kenaf: the forgotten fiber crop. The Australian New Crops Newsletter 10. Available at (accessed July 1998).


Genetic diversity analysis of kenaf (Hibiscus cannabinus L.) using AFLP (amplified fragment length polymorphism) markers

  • Rouxlene Coetzee (a1), Liezel Herselman (a1) and Maryke T. Labuschagne (a1)


Full text views

Total number of HTML views: 0
Total number of PDF views: 0 *
Loading metrics...

Abstract views

Total abstract views: 0 *
Loading metrics...

* Views captured on Cambridge Core between <date>. This data will be updated every 24 hours.

Usage data cannot currently be displayed