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Enhancement of dwarf wheat germplasm with high-yield potential derived from induced mutagenesis

Published online by Cambridge University Press:  29 December 2016

Hongchun Xiong ,
Huijun Guo ,
Yongdun Xie ,
Linshu Zhao ,
Jiayu Gu ,
Shirong Zhao ,
Junhui Li  and
Luxiang Liu
Hongchun Xiong
Affiliation:
Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, China
Huijun Guo
Affiliation:
Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, China
Yongdun Xie
Affiliation:
Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, China
Linshu Zhao
Affiliation:
Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, China
Jiayu Gu
Affiliation:
Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, China
Shirong Zhao
Affiliation:
Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, China
Junhui Li
Affiliation:
Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, China
Luxiang Liu*
Affiliation:
Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement, National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, China
*
*Corresponding author. E-mail: liuluxiang@caas.cn
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Abstract

The dwarfing wheat (Triticum aestivum L.) breeding has promoted a dramatic increase in yields. Since the utilized dwarfing genes in wheat are very limited, identification of novel dwarfing genes is necessary for improving the genetic diversity of wheat. In this study, more than 300 dwarfing wheat lines from induced mutation were screened by kompetitive allele-specific PCR and gibberellin (GA) treatment. The 17.49% of Rht-D1b and 1.37% of Rht-B1b dwarfing mutants were identified in this mutant group. Additionally, Rht-D1b mutants showed more effective in reduction of plant height and higher 1000-grain weight comparing with that of Rht-B1b mutants. By combing with comparison of yield components and expression profile of GA biosynthetic genes with wild-type, the GA-responsive mutant dm15 without directly involvement in GA metabolism, significantly increased 1000-grain weight but no change of other yield components in two locations of field experiments. Meanwhile, another elite mutant dm11 with change of GA biosynthetic genes expression was also identified. These mutants will be promising candidates for dwarfing wheat breeding.

Keywords

dwarfing mutantgibberellinRht-B1b/D1bwheatyield
Type
Research Article
Information
Plant Genetic Resources , Volume 16 , Issue 1 , February 2018 , pp. 74 - 81
Copyright
Copyright © NIAB 2016 

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Footnotes

†

These authors contributed equally to this work.

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