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Enhancement of dwarf wheat germplasm with high-yield potential derived from induced mutagenesis

  • Hongchun Xiong (a1), Huijun Guo (a1), Yongdun Xie (a1), Linshu Zhao (a1), Jiayu Gu (a1), Shirong Zhao (a1), Junhui Li (a1) and Luxiang Liu (a1)...


The dwarfing wheat (Triticum aestivum L.) breeding has promoted a dramatic increase in yields. Since the utilized dwarfing genes in wheat are very limited, identification of novel dwarfing genes is necessary for improving the genetic diversity of wheat. In this study, more than 300 dwarfing wheat lines from induced mutation were screened by kompetitive allele-specific PCR and gibberellin (GA) treatment. The 17.49% of Rht-D1b and 1.37% of Rht-B1b dwarfing mutants were identified in this mutant group. Additionally, Rht-D1b mutants showed more effective in reduction of plant height and higher 1000-grain weight comparing with that of Rht-B1b mutants. By combing with comparison of yield components and expression profile of GA biosynthetic genes with wild-type, the GA-responsive mutant dm15 without directly involvement in GA metabolism, significantly increased 1000-grain weight but no change of other yield components in two locations of field experiments. Meanwhile, another elite mutant dm11 with change of GA biosynthetic genes expression was also identified. These mutants will be promising candidates for dwarfing wheat breeding.


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Ahloowalia, BS and Maluszynski, M (2001) Induced mutations – a new paradigm in plant breeding. Euphytica 118: 167173.
Ahloowalia, BS, Maluszynski, M and Nichterlein, K (2004) Global impact of mutation-derived varieties. Euphytica 135: 187204.
Bazhenov, MS, Divashuk, MG, Amagai, Y, Watanabe, N and Karlov, GI (2015) Isolation of the dwarfing Rht-B1p (Rht17) gene from wheat and the development of an allele-specific PCR marker. Molecular Breeding 35: 123.
Botwright, TL, Rebetzke, GJ, Condon, AG and Richards, RA (2005) Influence of the gibberellin-sensitive Rht8 dwarfing gene on leaf epidermal cell dimensions and early vigour in wheat (Triticum aestivum L.). Annals of Botany 95: 631639.
Butler, JD, Byrne, PF, Mohammadi, V, Chapman, PL and Haley, SD (2005) Agronomic performance of Rht alleles in a spring wheat population across a range of moisture levels. Crop Science 45: 939947.
Chapman, SC, Mathews, KL, Trethowan, RM and Singh, RP (2007) Relationships between height and yield in near-isogenic spring wheats that contrast for major reduced height genes. Euphytica 157: 391397.
Chen, L, Phillips, AL, Condon, AG, Parry, MA and Hu, YG (2013) GA-responsive dwarfing gene Rht12 affects the developmental and agronomic traits in common bread wheat. PLoS ONE 8: e62285.
Chen, L, Hao, L, Condon, AG and Hu, YG (2014) Exogenous GA3 application can compensate the morphogenetic effects of the GA-responsive dwarfing gene Rht12 in bread wheat. PLoS ONE 9: e86431.
Claeys, H, De Bodt, S and Inzé, D (2014) Gibberellins and DELLAs: central nodes in growth regulatory networks. Trends in Plant Science 19: 231239.
Daoura, BG, Chen, L, Du, YY and Hu, YG (2014) Genetic effects of dwarfing gene Rht-5 on agronomic traits in common wheat (Triticum aestivum L.) and QTL analysis on its linked traits. Field Crops Research 156: 2229.
Ellis, M, Spielmeyer, W, Gale, K, Rebetzke, G and Richards, R (2002) “Perfect” markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat. Theoretical and Applied Genetics 105: 10381042.
Ellis, MH, Rebetzke, GJ, Azanza, F, Richards, RA and Spielmeyer, W (2005) Molecular mapping of gibberellin-responsive dwarfing genes in bread wheat. Theoretical and Applied Genetics 111: 423430.
Flintham, JE, Borner, A, Worland, AJ and Gale, MD (1997) Optimizing wheat grain yield: effects of Rht (gibberellin-insensitive) dwarfing genes. Journal of Agricultural Science 128: 1125.
Gasperini, D, Greenland, A, Hedden, P, Dreos, R, Harwood, W and Griffiths, S (2012) Genetic and physiological analysis of Rht8 in bread wheat: an alternative source of semi-dwarfism with a reduced sensitivity to brassinosteroids. Journal of Experimental Botany 63: 44194436.
Guo, Y, Kong, FM, Xu, YF, Zhao, Y, Liang, X, Wang, YY, An, DG and Li, SS (2012) QTL mapping for seedling traits in wheat grown under varying concentrations of N, P and K nutrients. Theoretical and Applied Genetics 124: 851865.
Hedden, P (2003) The genes of the green revolution. Trends in Genetics 19: 59.
Hou, P, Gao, Q, Xie, R, Li, S, Meng, Q, Kirkby, EA, Römheld, V, Müller, T, Zhang, F, Cui, Z and Chen, X (2012) Grain yields in relation to N requirement: optimizing nitrogen management for spring maize grown in China. Field Crops Research 129: 16.
Knopf, C, Becker, H, Ebmeyer, E and Korzun, V (2008) Occurrence of three dwarfing Rht genes in German winter wheat varieties. Cereal Research Communications 36: 553560.
Korzun, V, Röder, SM, Ganal, WM, Worland, JA and Law, NC (1998) Genetic analysis of the dwarfing gene (Rht8) in wheat. Part I. Molecular mapping of Rht8 on the short arm of chromosome 2D of bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics 96: 11041109.
Li, XP, Lan, SQ, Liu, YP, Gale, MD and Worland, TJ (2006) Effects of different Rht-B1b, Rht-D1b and Rht-B1c dwarfing genes on agronomic characteristics in wheat. Cereal Research Communications 34(2–3): 919924.
Li, A, Yang, W, Guo, X, Liu, D, Sun, J and Zhang, A (2012) Isolation of a gibberellin-insensitive dwarfing gene, Rht-B1e, and development of an allele-specific PCR marker. Molecular Breeding 30: 14431451.
Lou, X, Li, X, Li, A, Pu, M, Shoaib, M, Liu, D, Sun, J, Zhang, A and Yang, W (2016) The 160 bp insertion in the promoter of Rht-B1i plays a vital role in increasing wheat height. Frontiers in Plant Science 7: 307.
Pearce, S, Saville, R, Vaughan, SP, Chandler, PM, Wilhelm, EP, Sparks, CA, Al-Kaff, N, Korolev, A, Boulton, MI, Phillips, AL, Hedden, P, Nicholson, P and Thomas, SG (2011) Molecular characterization of Rht-1 dwarfing genes in hexaploid wheat. Plant Physiology 157: 18201831.
Pearce, S, Huttly, AK, Prosser, IM, Li, YD, Vaughan, SP, Gallova, B, Patil, A, Coghill, JA, Dubcovsky, J, Hedden, P and Phillips, AL (2015) Heterologous expression and transcript analysis of gibberellin biosynthetic genes of grasses reveals novel functionality in the GA3ox family. BMC Plant Biology 15: 130.
Peng, J, Richards, DE, Hartley, NM, Murphy, GP, Devos, KM, Flintham, JE, Beales, J, Fish, LJ, Worland, AJ, Pelica, F, Sudhakar, D, Christou, P, Snape, JW, Gale, MD and Harberd, NP (1999) ‘Green revolution’ genes encode mutant gibberellin response modulators. Nature 400(6741): 256261.
Rasheed, A, Wen, W, Gao, F, Zhai, S, Jin, H, Liu, J, Guo, Q, Zhang, Y, Dreisigacker, S, Xia, X and He, Z (2016) Development and validation of KASP assays for genes underpinning key economic traits in bread wheat. Theoretical and Applied Genetics 129: 18431860.
Rebetzke, GJ, Ellis, MH, Bonnett, DG, Mickelson, B, Condon, AG and Richards, RA (2012) Height reduction and agronomic performance for selected gibberellin-responsive dwarfing genes in bread wheat (Triticum aestivum L.). Field Crops Research 126: 8796.
Rebetzke, GJ, Richards, RA, Fischer, VM and Mickelson, BJ (1999) Breeding long coleoptile, reduced height wheats. Euphytica 106: 159168.
Rebetzke, GJ, Richards, RA, Sirault, XRR and Morrison, AD (2004) Genetic analysis of coleoptile length and diameter in wheat. Australian Journal of Agricultural Research 55: 733743.
Richards, RA (1992) The effect of dwarfing genes in spring wheat in dry environments. I. Agronomic characteristics. Crop Pasture Science 43: 517527.
Rieu, I, Ruiz-Rivero, O, Fernandez-Garcia, N, Griffiths, J, Powers, SJ, Gong, F, Linhartova, T, Eriksson, S, Nilsson, O, Thomas, SG, Phillips, AL and Hedden, P (2008) The gibberellin biosynthetic genes AtGA20ox1 and AtGA20ox2 act, partially redundantly, to promote growth and development throughout the Arabidopsis life cycle. Plant Journal 53: 488504.
Sakamoto, T and Matsuoka, M (2004) Generating high-yielding varieties by genetic manipulation of plant architecture. Current Opinion in Biotechnology 15: 144147.
Sasaki, A, Ashikari, M, Ueguchi-Tanaka, M, Itoh, H, Nishimura, A, Swapan, D, Ishiyama, K, Saito, T, Kobayashi, M, Khush, GS, Kitano, H and Matsuoka, M (2002) Green revolution: a mutant gibberellin-synthesis gene in rice. Nature 416: 701702.
Tang, N, Jiang, Y, He, BR and Hu, YG (2009) The effects of dwarfing genes (Rht-B1b, Rht-D1b, and Rht8) with different sensitivity to GA(3) on the coleoptile length and plant height of wheat. Agricultural Science in China 8: 10281038.
Teng, F, Zhai, LH, Liu, RX, Bai, W, Wang, LQ, Huo, DG, Tao, YS, Zheng, YL and Zhang, ZX (2013) ZmGA3ox2, a candidate gene for a major QTL, qPH3.1, for plant height in maize. Plant Journal 73: 405416.
Wang, YS, Chen, L, Du, YY, Yang, ZY, Condon, AG and Hu, YG (2014) Genetic effect of dwarfing gene Rht13 compared with Rht-D1b on plant height and some agronomic traits in common wheat (Triticum aestivum L.). Field Crops Research 162: 3947.
Yang, ZY, Zheng, JC, Liu, CY, Wang, YS, Condon, AG, Chen, YF and Hu, YG (2015) Effects of the GA-responsive dwarfing gene Rht18 from tetraploid wheat on agronomic traits of common wheat. Field Crops Research 183: 92101.
Zanke, CD, Ling, J, Plieske, J, Kollers, S, Ebmeyer, E, Korzun, V, Argillier, O, Stiewe, G, Hinze, M, Neumann, K, Ganal, MW and Roder, MS (2014) Whole genome association mapping of plant height in winter wheat (Triticum aestivum L.). PLoS ONE 9: e113287.
Zhang, XK, Yang, SJ, Zhou, Y, He, ZH and Xia, XC (2006) Distribution of the Rht-B1b, Rht-D1b and Rht8 reduced height genes in autumn-sown Chinese wheats detected by molecular markers. Euphytica 152: 109116.


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Enhancement of dwarf wheat germplasm with high-yield potential derived from induced mutagenesis

  • Hongchun Xiong (a1), Huijun Guo (a1), Yongdun Xie (a1), Linshu Zhao (a1), Jiayu Gu (a1), Shirong Zhao (a1), Junhui Li (a1) and Luxiang Liu (a1)...


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