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Characterization of novel low-molecular-weight glutenin subunit genes from the diploid wild wheat relative Aegilops umbellulata

Published online by Cambridge University Press:  12 May 2022

Wenyang Wang ,
Wenjun Ji ,
Lihua Feng ,
Shunzong Ning ,
Zhongwei Yuan ,
Ming Hao ,
Lianquan Zhang Open the ORCID record for Lianquan Zhang [Opens in a new window] ,
Zehong Yan ,
Bihua Wu  and
Dengcai Liu
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Wenyang Wang
Affiliation:
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang 611130, China Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
Wenjun Ji
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
Lihua Feng
Affiliation:
College of Agronomy, Sichuan Agricultural University, Wenjiang 611130, China
Shunzong Ning
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
Zhongwei Yuan
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
Ming Hao
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
Lianquan Zhang
Affiliation:
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang 611130, China Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
Zehong Yan
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
Bihua Wu
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
Dengcai Liu
Affiliation:
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang 611130, China Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
Lin Huang*
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, Wenjiang 611130, China
*
Author for correspondence: Lin Huang, E-mail: lhuang@sicau.edu.cn
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Abstract

Low molecular weight glutenin subunits (LWM-GSs) play a crucial role in determining wheat flour processing quality. In this work, 35 novel LMW-GS genes (32 active and three pseudogenes) from three Aegilops umbellulata (2n = 2x = 14, UU) accessions were amplified by allelic-specific PCR. We found that all LMW-GS genes had the same primary structure shared by other known LMW-GSs. Thirty-two active genes encode 31 typical LMW-m-type subunits. The MZ424050 possessed nine cysteine residues with an extra cysteine residue located in the last amino acid residue of the conserved C-terminal III, which could benefit the formation of larger glutenin polymers, and therefore may have positive effects on dough properties. We have found extensive variations which were mainly resulted from single-nucleotide polymorphisms (SNPs) and insertions and deletions (InDels) among the LMW-GS genes in Ae. umbellulata. Our results demonstrated that Ae. umbellulata is an important source of LMW-GS variants and the potential value of the novel LMW-GS alleles for wheat quality improvement.

Keywords

Aegilops umbellulataLMW-GSU genomewheat quality improvement
Type
Research Article
Information
Plant Genetic Resources , Volume 20 , Issue 1 , February 2022 , pp. 1 - 6
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of NIAB

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Footnotes

*

Wenyang Wang and Wenjun Ji have contributed equally to this work.

References

An, X, Zhang, Q, Yan, Y, Li, Q, Zhang, Y, Wang, A, Pei, Y, Tian, J, Wang, H, Hsam, SLK and Zeller, FJ (2006) Cloning and molecular characterization of three novel LMW-i glutenin subunit genes from cultivated einkorn (Triticum monococcum L.). Theoretical and Applied Genetics 113, 383–395.10.1007/s00122-006-0299-xCrossRefGoogle Scholar
Anderson, OD and Greene, FC (1989) The characterization and comparative analysis of high-molecular-weight glutenin genes from genomes A and B of a hexaploid bread wheat. Theoretical and Applied Genetics 77, 689–700.CrossRefGoogle Scholar
Bansal, M, Kaur, S, Dhaliwal, HS, Bains, NS, Bariana, HS, Chhuneja, P and Bansal, UK (2017) Mapping of Aegilops umbellulata-derived leaf rust and stripe rust resistance loci in wheat. Plant Pathology 66, 38–44.CrossRefGoogle Scholar
Chhuneja, P, Kaur, S, Goel, RK, Aghaee-Sarbaezeh, M, Parashar, M and Dhaliwal, HS (2008) Transfer of leaf rust and stripe rust resistance from Aegilops umbellulata Zhuk. to bread wheat (Triticum aestivum L.). Genetic Resources and Crop Evolution 55, 849–859.CrossRefGoogle Scholar
Dai, SF, Zhao, L, Xue, XF, Jia, YN, Liu, DC, Pu, ZJ, Zheng, YL and Yan, ZH (2015) Analysis of high-molecular-weight glutenin subunits in five amphidiploids and their parental diploid species Aegilops umbellulata and Aegilops uniaristata. Plant Genetic Resources 13, 186–189.CrossRefGoogle Scholar
D'Ovidio, R and Masci, S (2004) The low-molecular-weight glutenin subunits of wheat gluten. Journal of Cereal Science 39, 321–339.CrossRefGoogle Scholar
Edae, EA, Olivera, PD, Jin, Y, Poland, JA and Rouse, MN (2016) Genotype-by-sequencing facilitates genetic mapping of a stem rust resistance locus in Aegilops umbellulata, a wild relative of cultivated wheat. BMC Genomics 17, 1039.CrossRefGoogle ScholarPubMed
Gill, BS, Sharma, C, Raupp, WJ, Browder, LE, Heachett, JH, Harvey, TL, Moseman, JG and Waines, JG (1985) Evaluation of Aegilops species for resistance to wheat powdery mildew, wheat leaf rust, Hessian fly, and greenbug. Plant Disease 69, 314–316.Google Scholar
Hall, T (2007) BioEdit, Version 7.0.9. Carlsbad, CA: Computer Program and Documentation, lbis Biosciences.Google Scholar
Harberd, NP, Bartels, D and Thompson, RDM (1985) Analysis of the gliadin multigene loci in bread wheat using nullisomic-tetrasomic lines. Molecular Genetics and Genomics 198, 234–242.CrossRefGoogle Scholar
Henkrar, F, El-Haddoury, J, Iraqi, D, Bendaou, N and Udupa, SM (2017) Allelic variation at high-molecular weight and low-molecular weight glutenin subunit genes in Moroccan bread wheat and durum wheat cultivars. 3 Biotech 7, 287.CrossRefGoogle ScholarPubMed
Huang, L, He, Y, Jin, YR, Wang, F, He, JS, Feng, LH, Liu, DC and Wu, BH (2018) Characterization of novel LMW glutenin subunit genes at the Glu-M3 locus from Aegilops comosa. 3 Biotech 8, 379.CrossRefGoogle ScholarPubMed
Ikeda, TM, Nagamine, T, Fukuoka, H and Yano, H (2002) Identification of new low-molecular-weight glutenin subunit genes in wheat. Theoretical and Applied Genetics 104, 680–687.CrossRefGoogle ScholarPubMed
Islam-Faridi, MN (1988) Genetical Studies of Grain Protein and Developmental Characters in Wheat (PhD Thesis). University of Cambridge, Cambridge.Google Scholar
Lan, QX, Feng, B, Xu, ZB, Zhao, GJ and Wang, T (2013) Molecular cloning and characterization of five novel low molecular weight glutenin subunit genes from Tibetan wheat landraces (Triticum aestivum L.). Genetic Resources and Crop Evolution 60, 799–806.CrossRefGoogle Scholar
Law, CN and Payne, PI (1983) Genetical aspects of breeding for improved grain protein content and type in wheat. Journal of Cereal Science 1, 79–93.CrossRefGoogle Scholar
Lee, JY, Beom, HR, Altenbach, SB, Lim, SH, Kim, YT, Kang, CS, Yoon, UH, Gupta, R, Kim, ST, Ahn, SN and Kim, YM (2016) Comprehensive identification of LMW-GS genes and their protein products in a common wheat variety. Functional & Integrative Genomics 16, 269–279.CrossRefGoogle Scholar
Li, XH, Ma, W, Gao, LY, Zhang, YZ, Wang, AL, Ji, KM, Wang, K, Appels, R and Yan, Y (2008) A novel chimeric LMW-GS gene from the wild relatives of wheat Ae. kotschyi and Ae. juvenalis: evolution at the Glu-3 loci. Genetics 180, 93–101.10.1534/genetics.108.092403CrossRefGoogle ScholarPubMed
Li, XH, Wang, K, Wang, SL, Gao, LY, Xie, XX, Hsam, SLK, Zeller, FJ and Yan, YM (2010) Molecular characterization and comparative transcriptional analysis of LMW-m-type genes from wheat (Triticum aestivum L.) and Aegilops species. Theoretical and Applied Genetics 121, 845–856.CrossRefGoogle ScholarPubMed
Li, XJ, Liu, TH, Song, LJ, Zhang, H, Li, LQ and Gao, X (2016) Influence of high-molecular-weight glutenin subunit composition at Glu-A1 and Glu-D1 loci on secondary and micro structures of gluten in wheat (Triticum aestivum L.). Food Chemistry 213, 728–734.CrossRefGoogle Scholar
Liu, ZJ, Zhang, XM, Wan, YF, Liu, KF and Wang, DW (2002) Characterization of high-molecular-weight glutenin subunits and their coding genes from Aegilops umbellulata. Acta Botanica Sinica 44, 809–814.Google Scholar
Rogers, SO and Bendich, AJ (1985) Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Molecular Biology 5, 69–76.CrossRefGoogle ScholarPubMed
Schachermayr, GM, Siedler, H, Gale, MD, Winzeller, H, Winzeller, M and Keller, B (1994) Identification and localization of molecular markers linked to the Lr9 leaf rust resistance gene of wheat. Theoretical and Applied Genetics 88, 110–115.CrossRefGoogle Scholar
Song, ZP, Dai, SF, Jia, YN, Zhao, L, Kang, LZ, Liu, DC, Wei, YM, Zheng, YL and Yan, ZH (2019) Development and characterization of Triticum turgidum–Aegilops umbellulata amphidiploids. Plant Genetic Resources 17, 24–32.CrossRefGoogle Scholar
Tamura, K, Stecher, G, Peterson, D, Filipski, A and Kumar, S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30, 2725–2729.CrossRefGoogle ScholarPubMed
Wang, SL, Li, XH, Wang, K, Wang, XZ, Li, SS, Zhang, YZ, Guo, GF, Zeller, FJ, Hsam, SLK and Yan, YM (2011 a) Phylogenetic analysis of C, M, N and U genomes and their relationships with Triticum and other related genomes as revealed by LMW-GS genes at Glu-3 loci. Genome 54, 273–284.CrossRefGoogle ScholarPubMed
Wang, K, Gao, L, Wang, S, Zhang, Y, Li, X, Zhang, M, Xie, Z, Yan, Y, Belgard, M and Ma, W (2011 b) Phylogenetic relationship of a new class of LMW-GS genes in the M genome of Aegilops comosa. Theoretical and Applied Genetics 122, 1411–1425.10.1007/s00122-011-1541-8CrossRefGoogle Scholar
Wang, J, Wang, C, Zhen, S, Li, X and Yan, Y (2018) Low-molecular-weight glutenin subunits from the 1U genome of Aegilops umbellulata confer superior dough rheological properties and improve bread making quality of bread wheat. Journal of the Science of Food and Agriculture 98, 2156–2167.CrossRefGoogle Scholar
Xiang, L, Huang, L, Gong, FY, Liu, J, Wang, YF, Jin, YR, He, Y, He, JS, Jiang, QT, Zheng, YL, Liu, DC and Wu, BH (2019) Enriching LMW-GS alleles and strengthening gluten properties of common wheat through wide hybridization with wild emmer. 3 Biotech 9, 355.CrossRefGoogle ScholarPubMed
Xu, H, Wang, RJ, Shen, X, Zhao, YL, Sun, GL, Zhao, HX and Guo, AG (2006) Functional properties of a new low-molecular-weight glutenin subunit gene from a bread wheat cultivar. Theoretical and Applied Genetics 113, 1295–1303.10.1007/s00122-006-0383-2CrossRefGoogle ScholarPubMed
Zhang, Y, Li, Q, Yan, Y, Zheng, J, An, X, Xiao, Y, Wang, A, Wang, H, Hsam, SLK and Zeller, FJ (2006) Molecular characterization and phylogenetic analysis of a novel glutenin gene (Dy10.1t) from Aegilops tauschii. Genome 49, 735–745.CrossRefGoogle ScholarPubMed
Zhang, XF, Liu, DC, Zhang, JH, Jiang, W, Luo, GB, Yang, WL, Sun, JZ, Tong, YP, Cui, DQ and Zhang, AM (2013) Novel insights into the composition, variation, organization, and expression of the low-molecular-weight glutenin subunit gene family in common wheat. Journal of Experimental Botany 64, 2027–2040.CrossRefGoogle ScholarPubMed
Zhao, H, Wang, R, Guo, A, Hu, S and Sun, G (2004) Development of primers specific for LMW-GS genes located on chromosome 1D and molecular characterization of a gene from Glu-D3 complex locus in bread wheat. Hereditas 141, 193–198.10.1111/j.1601-5223.2004.01852.xCrossRefGoogle ScholarPubMed
Zhu, ZD, Zhou, RH, Kong, XY, Dong, YC and Jia, JZ (2006) Microsatellite marker identification of a Triticum aestivum-Aegilops umbellulata substitution line with powdery mildew resistance. Euphytica 150, 149–153.CrossRefGoogle Scholar
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